The impact of the international rice genebank (IRG) on rice farming in Bangladesh

The International Rice Genebank (IRG) currently safeguards the largest and most diverse collection of rice genetic resources in the world. Over the past decades, genetic resources from the IRG have been used efectively to increase smallholder farmers’ rice productivity in developing economies. Bangladesh is one of the direct and indirect recipients of IRG germplasm for rice genetic improvement. This study aimed to map the impact pathways of IRG germplasm transfers to Bangladesh, evaluate the genetic contribution of IRG germplasms to rice productivity of farmers, and compute the equivalent economic beneft

Disentangling Challenges to Scaling Alternate Wetting and Drying Technology for Rice Cultivation: Distilling Lessons From 20 Years of Experience in the Philippines

Alternate wetting and drying (AWD) is a low-cost innovation that enables farmers to adapt to increasingly water scarcity conditions (such as drought), increase overall farm production efficiency, and mitigate greenhouse gas (GHG) emissions. It is seen as a pathway for transforming agri-food systems into more resilient, productive, biologically diverse, and equitable forms, ensuring our commitments to the UN Sustainable Development Goals (SDGs). This paper uses scaling up and innovation uncertainty frameworks to review the success and challenges of AWD's 20-year scaling trajectory in the Philippines and explain the key factors that have influenced its outcomes. The framework adapted for this study is also used to examine the fitness between the scaling context and requirements, organizational mission, and corresponding capabilities. Findings show the innovation platform that vertically integrated key actors and locally adapted AWD has helped foster essential breakthroughs in creating an enabling environment that took AWD to national policy adoption in the Philippines. However, the dominant focus on technology transfer, product focus, and preference for controlled environments in the scaling practice has neglected many important contextual factors, allowing mismatches in enabling policy incentives, institutions, and scale to diminish the impacts of AWD in gravity-based systems. Our findings suggest that rethinking and re-envisioning the ways in which the impact can be scaled in irrigation rice systems using AWD is critical to sustaining food security and making the agriculture sector more resilient to climate change

OPTIMIZING WATER PROVISIONING SERVICES OF FOREST PLANTATIONS IN WATER SCARCE AREAS: A COST-BENEFIT ANALYSIS OF REFORESTATION APPROACHES IN MAASIN WATERSHED FOREST RESERVE, ILOILO

Master'sMASTER OF SCIENCE (ENVIRONMENTAL MANAGEMENT) (MEM

Guidelines for the use and interpretation of assays for monitoring autophagy

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field