Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes.

Ribosomes synthesizing nascent secretory proteins are targeted to the membrane by the signal recognition particle (SRP), a small ribonucleoprotein that binds to the signal peptide as it emerges from the ribosome. SRP arrests further elongation, causing ribosomes to stack behind the arrested ribosome. Upon interaction of SRP with its receptor on the ER membrane, the translation arrest is released and the ribosome becomes bound to the ER membrane. We have examined the distribution of unattached and membrane-bound ribosomes during the translation of mRNAs encoding two secretory proteins, bovine preprolactin and rat preproinsulin I. We find that the enhancement of ribosome stacking that occurs when SRP arrests translation of these proteins is relaxed in the presence of microsomal membranes. We also demonstrate that two previously described populations of membrane-associated ribosomes, distinguished by their sensitivity to high salt or EDTA extraction, correspond to ribosomes that have synthesized differing lengths of the nascent polypeptide. This analysis has revealed that nascent chain insertion into the membrane begins at distinct points for different presecretory proteins

Outside and As If

The invitation to write this article was accompanied by a sketch definition of narrative architecure which included the suggestion ... Many architects have something to say in their architecture, a story to tell. There are a variety of means architects employ in expressing their own, or their clients values, thoughts, wishes, beliefs, and desires..

New method for critical failure prediction of complex systems

Rigorous analytical technique, called criticality determination methodology /or CD technique/ determines the probability that a given complex system will successfully achieve stated objectives. The CD technique identifies critical elements of the system by a failure mode and effects analysis

Trauma Informed Community Building Evaluation: A Formative Evaluation of the TICB Model and its Implementation in Potrero Hill

This formative evaluation of the TICB model and its implementation in the PTA public housing community was conducted between September 2014 and July 2015 by an evaluation team from the HOPE SF Learning Center. This evaluation was designed to support the further development of the TICB model as well as inform efforts to implement the model in Potrero Terrace and Annex and other communities. This evaluation seeks to examine the implementation and impact of the TICB model at PTA in order to: * Understand the impact of ongoing TICB-informed programming through analysis of outputs and outcomes prioritized by stakeholder partners [see Appendix A]. * Identify facilitators and barriers to implementation of the TICB model in community-building work within PTA and the surrounding Potrero Hill neighborhood. * Inform BRIDGE Housing's work to improve programming, and guide future program priorities and structures. * Generate information to better understand the impact of the financial investment in helping to build community with and between public housing residents and residents of the surrounding neighborhood. * Assess implications for replicability/reproducibility at other public housing communities, including the additional HOPE SF sites, and beyond

Poems/photographs/printing: a handmade book by Jeffrey A. Wolin

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