Analysis of proteins synthesized by 9.5 day mouse embryos: Determination of cardiac and noncardiac proteins

To catalog polypeptides that were specific to developing hearts, we separated 35 S-methionine-labeled 9.5 day mouse embyros into cardiac and noncardiac (carcass) components. Two-dimensional gels were then used to analyze the polypeptides synthesized in these two fractions. As a result, we were able to distinguish polypeptides that were specific to or increased in the heart as well as those polypeptides that were specific to or inceased in the embryo minus the dissected heart. Using this analysis, there were two polypeptides that were cardiac-specific and 17 that were expressed at increased levels by at least twofold in the heart. The cardiac-specific polypeptides may be used in further studies to identify early cardiac tissue. Conversely, there were 26 polypeptides unique to noncardiac structures and an additional 15 that were increased in the carcass more than twofold relative to the heart. The noncardiac-specific polypeptides may be used to define contamination of putative cardiac tissue with noncardiac material. Two of the polypeptides expressed more abundantly in the carcass appeared to correspond to known proteins in the mouse fibroblast database, cyclin and tropomyosin 4. Thus the heart at 9.5 days of murine development can be distinguished readily from the remainder of the embryonic mouse both macroscopically and on two-dimensional gels.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50419/1/1080290209_ftp.pd

Analysis of proteins expressed at the time of murine organogenesis

Two-dimensional electrophoretograms were prepared from wild-type C57BL/6J embryos from day 7.5 through day 9.0 of development. This time period encompasses a critical window of development as the embryo traverses from an egg cylinder through major organogenesis. Consequently, we term this resource MOPED (for mouse organogenesis protein electrophoresis database). By resolving and analyzing the behavior of approximately 1,000 polypeptides per time point, we were able to track many of these polypeptides through this time period in development. Of special note was a burst of induced protein synthesis that was observed on day 8.5 of development. Polypeptides observed in mouse embryos that match those identified previously in mouse fibroblasts were noted. Two of them (the intermediate filament-associated protein and tropomyosin-4) were significantly altered in 8.5 day embryos. As more polypeptides are designated, it will be possible to expand the known proteins in the database. MOPED establishes the patterns of synthesis of a large number of polypeptides during a crucial period of development. Thus MOPED is designed to analyze proteins relevant to mouse embryogenesis in the future.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50418/1/1080290207_ftp.pd

The Relationship of Anthropometric Ratios and Kinematic & Kinetic Measures of the Hip in Recreational Male Athletes Performing the Back Squat

The back squat exercise is a popular resistance exercise that involves synchronous movement of the entire body to perform the movement properly . The two main focuses when considering engaging in the back squat exercise are the risks of injury and the enhancement of performance. There is little information and scant understanding on the way body proportionality impacts the risk of injury and performance. The present study investigated this issue by investigating if there was a relationship between anthropometric ratios and kinematic & kinetic outcomes from the left and right hip when experienced, male back squatters performed a single repetition at 80% of 1RM load. Additionally, this study compared the kinematic & kinetic outcomes of the left and right hip. The results of this study showed that amongst experienced, male back squatters, there was no relationship of kinematic and kinetic outcomes to the anthropometric ratios. The results did indicate that there were significant differences between the outcomes of the left and right hip in four of the twelve comparisons. There was little variance amongst the anthropometric ratios of the subjects and they were an experienced group of back squatters. These two factors are believed to have impacted the results. Future studies could use subjects of more varied anthropometric ratios and subjects with less experience

Guillermo del Toro’s The Shape of Water (2017): Trump Era Update of Cold War Creature from the Black Lagoon (1954) and Civil War Reckoning El laberinto del fauno (2006).

Del Toro's latest film uses an amphibian creature dragged from the Amazon to reflect on the current situation of immigrants in this country, especially those from south of the border. In the process, the director uses Trump's nationalist and racist framing of the immigration debate as an opportunity to remake a film he has loved since childhood, "Creature From the Black Lagoon." He places his film in 1962, during the Cuban Missile Crisis, but he insists that he has made a film that, although it takes place in the past, talks about the present. He also reworks a number of the themes and characterizations from his 2006 Spanish-language film "El laberinto del fauno." Both films, one situated in Franco's Spain shortly after the Civil War, and the other, in J.F.K.'s Camelot, nonetheless present equally chilling visions of systems of political power that foster brutal, authoritarian, and unforgiving social behaviors

The Relationship of Anthropometric Ratios and Kinematic & Kinetic Measures of the Hip in Recreational Male Athletes Performing the Back Squat

The back squat exercise is a popular resistance exercise that involves synchronous movement of the entire body to perform the movement properly . The two main focuses when considering engaging in the back squat exercise are the risks of injury and the enhancement of performance. There is little information and scant understanding on the way body proportionality impacts the risk of injury and performance. The present study investigated this issue by investigating if there was a relationship between anthropometric ratios and kinematic & kinetic outcomes from the left and right hip when experienced, male back squatters performed a single repetition at 80% of 1RM load. Additionally, this study compared the kinematic & kinetic outcomes of the left and right hip. The results of this study showed that amongst experienced, male back squatters, there was no relationship of kinematic and kinetic outcomes to the anthropometric ratios. The results did indicate that there were significant differences between the outcomes of the left and right hip in four of the twelve comparisons. There was little variance amongst the anthropometric ratios of the subjects and they were an experienced group of back squatters. These two factors are believed to have impacted the results. Future studies could use subjects of more varied anthropometric ratios and subjects with less experience

Carbon and sulfur cycling through geologic time

Mathematical models of the coupled global systems of sedimentary reservoirs and fluxes are used to infer variations in reservoir sizes and rates of sedimentation over periods of hundreds of millions of years. Perhaps most interesting is the coupled sulfide/sulfate carbon/carbonate system that controls global oxygen and carbon dioxide production and consumption is discussed

Scoping Review of Positive Mental Health Research for Students in Vocational Education and Training

Context: In this scoping review, we examine the knowledge base concerning positive mental health studies for students in vocational education and training (VET). The VET student population embraces approximately 30-52% of secondary school students in the Nordic countries, and 40% of the global student population. The risk of early school leaving (ESL) is substantially higher in VET than in general education and mental health may be a relevant factor in this matter. Yet, an overview of mental health studies in VET is lacking and therefore, this article aims to map empirical research studies that have explored positive mental health in VET students. The positive mental health framework, with its origin in Antonovsky's (2002) salutogenesis and positive psychology, focuses on factors that promote mental health and wellbeing rather than taking on a pathological perspective.  Methods: For our scoping review, we searched four databases, and 19 articles were found eligible for inclusion. These articles were systematically screened by means of a coding scheme to identify the following information: Country of origin of the study, its aim, research design, measures, conceptualization of mental health, and main findings.  Results: The evidence suggests that positive mental health is understood as a multifaceted concept, and wellbeing is the dimension that is explored most often, followed by resilience and quality of life. The majority of the included studies used a validated questionnaire to assess various aspects of positive mental health, and most of them sought to explore correlations between different dimensions of positive mental health. Main findings of the studies suggest that a supportive school environment, physical activity, and a strong vocational identity may contribute to positive mental health for students in VET. Furthermore, correlations have also been identified between environmental factors and positive mental health. Finally, findings from the review illustrate how even small-scale interventions may have far-reaching effects, due to the interrelatedness of the different dimensions within the positive mental health construct.  Conclusion: Findings from this review illustrate that numerous factors may affect the wellbeing of students in VET. In particular, a strong vocational identity, a supportive school environment, and physical activity may contribute to positive mental health. These findings suggest that VET teachers may promote the wellbeing of their students by providing a supportive psychosocial learning environment at school.

Scoping review of positive mental health research for students in vocational education and training

Context: In this scoping review, we examine the knowledge base concerning positive mental health studies for students in vocational education and training (VET). The VET student population embraces approximately 30-52% of secondary school students in the Nordic countries, and 40% of the global student population. The risk of early school leaving (ESL) is substantially higher in VET than in general education and mental health may be a relevant factor in this matter. Yet, an overview of mental health studies in VET is lacking and therefore, this article aims to map empirical research studies that have explored positive mental health in VET students. The positive mental health framework, with its origin in Antonovsky\u27s (2002) salutogenesis and positive psychology, focuses on factors that promote mental health and wellbeing rather than taking on a pathological perspective. Methods: For our scoping review, we searched four databases, and 19 articles were found eligible for inclusion. These articles were systematically screened by means of a coding scheme to identify the following information: Country of origin of the study, its aim, research design, measures, conceptualization of mental health, and main findings. Results: The evidence suggests that positive mental health is understood as a multifaceted concept, and wellbeing is the dimension that is explored most often, followed by resilience and quality of life. The majority of the included studies used a validated questionnaire to assess various aspects of positive mental health, and most of them sought to explore correlations between different dimensions of positive mental health. Main findings of the studies suggest that a supportive school environment, physical activity, and a strong vocational identity may contribute to positive mental health for students in VET. Furthermore, correlations have also been identified between environmental factors and positive mental health. Finally, findings from the review illustrate how even small-scale interventions may have far-reaching effects, due to the interrelatedness of the different dimensions within the positive mental health construct. Conclusion: Findings from this review illustrate that numerous factors may affect the wellbeing of students in VET. In particular, a strong vocational identity, a supportive school environment, and physical activity may contribute to positive mental health. These findings suggest that VET teachers may promote the wellbeing of their students by providing a supportive psychosocial learning environment at school. (DIPF/Orig.