Subaru and Gemini High Spatial Resolution Infrared 18 Micron Imaging Observations of Nearby Luminous Infrared Galaxies

We present the results of a ground-based, high spatial resolution infrared 18 micron imaging study of nearby luminous infrared galaxies (LIRGs), using the Subaru 8.2-m and Gemini South 8.1-m telescopes. The diffraction-limited images routinely achieved with these telescopes in the Q-band (17-23 micron) allow us to investigate the detailed spatial distribution of infrared emission in these LIRGs. We then investigate whether the emission surface brightnesses are modest, as observed in starbursts, or are so high that luminous active galactic nuclei (AGNs; high emission surface brightness energy sources) are indicated. The sample consists of 18 luminous buried AGN candidates and starburst-classified LIRGs identified in earlier infrared spectroscopy. We find that the infrared 18 micron emission from the buried AGN candidates is generally compact, and the estimated emission surface brightnesses are high, sometimes exceeding the maximum value observed in and theoretically predicted for a starburst phenomenon. The starburst-classified LIRGs usually display spatially extended 18 micron emission and the estimated emission surface brightnesses are modest, within the range sustained by a starburst phenomenon. The general agreement between infrared spectroscopic and imaging energy diagnostic methods suggests that both are useful tools for understanding the hidden energy sources of the dusty LIRG population.Comment: 17 pages, 3 figures, accepted for publication in AJ (No. 141, 2011 May issue). Higher resolution version is available at http://optik2.mtk.nao.ac.jp/~imanishi/Paper/20um/20um.pd

Physical properties of the circumnuclear starburst ring in the barred Galaxy NGC 1097

We report high resolution 12CO(J = 2-1), 13CO(J = 2-1), and 12CO(J = 3-2) imaging of the Seyfert 1/starburst ring galaxy NGC 1097 with the Submillimeter Array to study the physical and kinematic properties of the 1-kpc circumnuclear starburst ring. Individual star clusters as detected in the HST map of Pa_alpha line emission have been used to determine the star formation rate, and are compared with the properties of the molecular gas. The molecular ring has been resolved into individual clumps at GMA-scale of 200--300 pc in all three CO lines. The intersection between the dust lanes and the starburst ring, which is associated with orbit-crowding region, is resolved into two physically/kinematically distinct features in the 1.5x1.0 (105x70 pc) 12CO(J = 2-1) map. The clumps associated with the dust lanes have broader line width, higher surface gas density, and lower star formation rate, while the narrow line clumps associated with the starburst ring have opposite characteristics. Toomre-Q value under unity at the radius of the ring suggests that the molecular ring is gravitationally unstable to fragment at the scale of the GMA. The line widths and surface density of gas mass of the clumps show an azimuthal variation related to the large scale dynamics. The star formation rate, on the other hand, is not significantly affected by the dynamics, but has a correlation with the intensity ratio of 12CO (J = 3-2) and 12CO(J = 2-1), which traces the denser gas associated with star formation. Our resolved CO map, especially in the orbit-crowding region, for the first time demonstrates observationally that the physical/kinematic properties of the GMAs are affected by the large scale bar-potential dynamics in NGC 1097.Comment: 26 pages, 18 figures, accepted by Ap

High Glucose-Mediated Oxidative Stress Impairs Cell Migration

Deficient wound healing in diabetic patients is very frequent, but the cellular and molecular causes are poorly defined. In this study, we evaluate the hypothesis that high glucose concentrations inhibit cell migration. Using CHO.K1 cells, NIH-3T3 fibroblasts, mouse embryonic fibroblasts and primary skin fibroblasts from control and diabetic rats cultured in 5 mM D-glucose (low glucose, LG), 25 mM D-glucose (high glucose, HG) or 25 mM L-glucose medium (osmotic control - OC), we analyzed the migration speed, protrusion stability, cell polarity, adhesion maturation and the activity of the small Rho GTPase Rac1. We also analyzed the effects of reactive oxygen species by incubating cells with the antioxidant N-Acetyl-Cysteine (NAC). We observed that HG conditions inhibited cell migration when compared to LG or OC. This inhibition resulted from impaired cell polarity, protrusion destabilization and inhibition of adhesion maturation. Conversely, Rac1 activity, which promotes protrusion and blocks adhesion maturation, was increased in HG conditions, thus providing a mechanistic basis for the HG phenotype. Most of the HG effects were partially or completely rescued by treatment with NAC. These findings demonstrate that HG impairs cell migration due to an increase in oxidative stress that causes polarity loss, deficient adhesion and protrusion. These alterations arise, in large part, from increased Rac1 activity and may contribute to the poor wound healing observed in diabetic patients

Pan-cancer analysis of whole genomes

Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale(1-3). Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4-5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter(4); identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation(5,6); analyses timings and patterns of tumour evolution(7); describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity(8,9); and evaluates a range of more-specialized features of cancer genomes(8,10-18).Peer reviewe

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure

Notes for genera: basal clades of Fungi (including Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota)

Compared to the higher fungi (Dikarya), taxonomic and evolutionary studies on the basal clades of fungi are fewer in number. Thus, the generic boundaries and higher ranks in the basal clades of fungi are poorly known. Recent DNA based taxonomic studies have provided reliable and accurate information. It is therefore necessary to compile all available information since basal clades genera lack updated checklists or outlines. Recently, Tedersoo et al. (MycoKeys 13:1--20, 2016) accepted Aphelidiomycota and Rozellomycota in Fungal clade. Thus, we regard both these phyla as members in Kingdom Fungi. We accept 16 phyla in basal clades viz. Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota. Thus, 611 genera in 153 families, 43 orders and 18 classes are provided with details of classification, synonyms, life modes, distribution, recent literature and genomic data. Moreover, Catenariaceae Couch is proposed to be conserved, Cladochytriales Mozl.-Standr. is emended and the family Nephridiophagaceae is introduced

Endocytosis Genes Facilitate Protein and Membrane Transport in C. elegans Sensory Cilia

SummaryBackgroundMultiple intracellular transport pathways drive the formation, maintenance, and function of cilia, a compartmentalized organelle associated with motility, chemo-/mechano-/photosensation, and developmental signaling. These pathways include cilium-based intraflagellar transport (IFT) and poorly understood membrane trafficking events. Defects in ciliary transport contribute to the etiology of human ciliary disease such as Bardet-Biedl syndrome (BBS). In this study, we employ the genetically tractable nematode Caenorhabditis elegans to investigate whether endocytosis genes function in cilium formation and/or the transport of ciliary membrane or ciliary proteins.ResultsHere we show that localization of the clathrin light chain, AP-2 clathrin adaptor, dynamin, and RAB-5 endocytic proteins overlaps with a morphologically discrete periciliary membrane compartment associated with sensory cilia. In addition, ciliary transmembrane proteins such as G protein-coupled receptors concentrate at periciliary membranes. Disruption of endocytic gene function causes expansion of ciliary and/or periciliary membranes as well as defects in the ciliary targeting and/or transport dynamics of ciliary transmembrane and IFT proteins. Finally, genetic analyses reveal that the ciliary membrane expansions in dynamin and AP-2 mutants require bbs-8 and rab-8 function and that sensory signaling and endocytic genes may function in a common pathway to regulate ciliary membrane volume.ConclusionsThese data implicate C. elegans endocytosis proteins localized at the ciliary base in regulating ciliary and periciliary membrane volume and suggest that membrane retrieval from these compartments is counterbalanced by BBS-8 and RAB-8-mediated membrane delivery