Direct Numerical Simulations of the Compressible Low Atwood Rayleigh-Taylor Instability

Two fluids are considered Rayleigh-Taylor unstable when the more dense fluid is suspended above the less dense fluid in the presence of a gravitational like accelerative force. When a pertur- bation is applied to the interface between the two, they begin mixing as the light fluid rises and the heavy fluid drops. The extension of this to the compressible regime leads to the densities of the fluids to not be constant, but instead the molar mass is used to define the weights. At the interface, a density jump still occurs, but away from the interface the densities can vary in a variety of ways. This research investigates the effects of these density changes by imposing an initial background stratification before the initial perturbation is applied. Isothermal, isentropic, and isopycnic initial conditions are imposed for small molar mass differences and the growth of the perturbations are studied through the use of the Parallel Adaptive Wavelet Collocation Method. Strong non-linear interactions lead to the growth and destruction of complex vortical fields resulting in the initial suppression of the instability in all cases. For the isothermal case, this leads to a complete sup- pression at moderate to high Mach numbers, but for the isentropic and isopycnic case, this initial suppression is overcome and an increased acceleration occurs that grows with the Mach number. To further investigate these interactions, the vorticity transport equation is investigated and modified to give insights into the effects background stratification and fluctuating terms. In addition to this, a comparison is drawn to the simplified case of vortex pairs and rings propagating in the same stratifications. All of these interactions and discoveries have strong implications for understanding the physics governing engineering scenarios such as fuel capsules in inertial confinement fusion, flame front propagation in supernovae, and the mixing of fuels in some specialized burners

Dating the Cryptococcus gattii Dispersal to the North American Pacific Northwest.

The emergence of Cryptococcus gattii, previously regarded as a predominantly tropical pathogen, in the temperate climate of the North American Pacific Northwest (PNW) in 1999 prompted several questions. The most prevalent among these was the timing of the introduction of this pathogen to this novel environment. Here, we infer tip-dated timing estimates for the three clonal C. gattii populations observed in the PNW, VGIIa, VGIIb, and VGIIc, based on whole-genome sequencing of 134 C. gattii isolates and using Bayesian evolutionary analysis by sampling trees (BEAST). We estimated the nucleotide substitution rate for each lineage (1.59 × 10-8, 1.59 × 10-8, and 2.70 × 10-8, respectively) to be an order of magnitude higher than common neutral fungal mutation rates (2.0 × 10-9), indicating a microevolutionary rate (e.g., successive clonal generations in a laboratory) in comparison to a species' slower, macroevolutionary rate (e.g., when using fossil records). The clonal nature of the PNW C. gattii emergence over a narrow number of years would therefore possibly explain our higher mutation rates. Our results suggest that the mean time to most recent common ancestor for all three sublineages occurred within the last 60 to 100 years. While the cause of C. gattii dispersal to the PNW is still unclear, our research estimates that the arrival is neither ancient nor very recent (i.e., <25 years ago), making a strong case for an anthropogenic introduction. IMPORTANCE The recent emergence of the pathogenic fungus Cryptococcus gattii in the Pacific Northwest (PNW) resulted in numerous investigations into the epidemiological and enzootic impacts, as well as multiple genomic explorations of the three primary molecular subtypes of the fungus that were discovered. These studies lead to the general conclusion that the subtypes identified likely emerged out of Brazil. Here, we conducted genomic dating analyses to determine the ages of the various lineages seen in the PNW and propose hypothetical causes for the dispersal events. Bayesian evolutionary analysis strongly suggests that these independent fungal populations in the PNW are all 60 to 100 years old, providing a timing that is subsequent to the opening of the Panama Canal, which allowed for more direct shipping between Brazil and the western North American coastline, a possible driving event for these fungal translocation events

Journeys through the Golgi—taking stock in a new era

The Golgi apparatus is essential for protein sorting and transport. Many researchers have long been fascinated with the form and function of this organelle. Yet, despite decades of scrutiny, the mechanisms by which proteins are transported across the Golgi remain controversial. At a recent meeting, many prominent Golgi researchers assembled to critically evaluate the core issues in the field. This report presents the outcome of their discussions and highlights the key open questions that will help guide the field into a new era

Borrelia recurrentis employs a novel multifunctional surface protein with anti-complement, anti-opsonic and invasive potential to escape innate immunity

Borrelia recurrentis, the etiologic agent of louse-borne relapsing fever in humans, has evolved strategies, including antigenic variation, to evade immune defence, thereby causing severe diseases with high mortality rates. Here we identify for the first time a multifunctional surface lipoprotein of B. recurrentis, termed HcpA, and demonstrate that it binds human complement regulators, Factor H, CFHR-1, and simultaneously, the host protease plasminogen. Cell surface bound factor H was found to retain its activity and to confer resistance to complement attack. Moreover, ectopic expression of HcpA in a B. burgdorferi B313 strain, deficient in Factor H binding proteins, protected the transformed spirochetes from complement-mediated killing. Furthermore, HcpA-bound plasminogen/plasmin endows B. recurrentis with the potential to resist opsonization and to degrade extracellular matrix components. Together, the present study underscores the high virulence potential of B. recurrentis. The elucidation of the molecular basis underlying the versatile strategies of B. recurrentis to escape innate immunity and to persist in human tissues, including the brain, may help to understand the pathological processes underlying louse-borne relapsing fever

Writing in Britain and Ireland, c. 400 to c. 800

No abstract available

Simulations of beam-fueled supershot-like plasmas near ignition

Centrally peaked profiles would be advantageous for tokamak reactors since the pressure and the bootstrap current would be peaked in the regions of intense reactivity. We use the TRANSP plasma analysis code to investigate the feasibility of fueling with neutral beam injection. We show that for certain conditions, neutral beams with energies less than 120 keV can penetrate into the cores of plasmas that have a large thermonuclear yield and Q[sub DT] [approximately]10. This value of ODT is too small for an economical reactor if the neutral beam injection is the only fueling source. We give examples for several proposed compact prototype reactor designs

Antihyperalgesia by α2-GABAA Receptors Occurs Via a Genuine Spinal Action and Does Not Involve Supraspinal Sites

Drugs that enhance GABAergic inhibition alleviate inflammatory and neuropathic pain after spinal application. This antihyperalgesia occurs mainly through GABAA receptors (GABAARs) containing α2 subunits (α2-GABAARs). Previous work indicates that potentiation of these receptors in the spinal cord evokes profound antihyperalgesia also after systemic administration, but possible synergistic or antagonistic actions of supraspinal α2-GABAARs on spinal antihyperalgesia have not yet been addressed. Here we generated two lines of GABAAR-mutated mice, which either lack α2-GABAARs specifically from the spinal cord, or, which express only benzodiazepine-insensitive α2-GABAARs at this site. We analyzed the consequences of these mutations for antihyperalgesia evoked by systemic treatment with the novel non-sedative benzodiazepine site agonist HZ166 in neuropathic and inflammatory pain. Wild-type mice and both types of mutated mice had similar baseline nociceptive sensitivities and developed similar hyperalgesia. However, antihyperalgesia by systemic HZ166 was reduced in both mutated mouse lines by about 60% and was virtually indistinguishable from that of global point-mutated mice, in which all α2-GABAARs were benzodiazepine insensitive. The major (α2-dependent) component of GABAAR-mediated antihyperalgesia was therefore exclusively of spinal origin, whereas supraspinal α2-GABAARs had neither synergistic nor antagonistic effects on antihyperalgesia. Our results thus indicate that drugs that specifically target α2-GABAARs exert their antihyperalgesic effect through enhanced spinal nociceptive control. Such drugs may therefore be well-suited for the systemic treatment of different chronic pain conditions

Viewing Meaningful Work Through the Lens of Time

Authors have paid considerable attention to how to define the meaningful work construct. This has led to providing comprehensive definitions in the light of different theoretical frameworks that reflect a degree of contestation within the field. Several of them have proposed definitions linked to the individuals\u2019 pervasive sense of the value of their work. Others have offered descriptions centered on their temporal, episodic nature and emphasizing the individual\u2019s occasional work experience. These definitions reflected a potential temporal condition as well as the variety of time perspectives underpinning the authors\u2019 conceptualizations of the construct. This paper conducted a broad literature review to analyze works that have adopted a temporal framework or supported a time-based definition of the construct. The analysis indicates two different conceptualizations of the construct: as a permanent/steady mindset and as a changeable/episodic experience. As a reflective paper, the present contribution develops an overall framework for views and theories on meaningful work. It reports a critical review on the matter to elevate understanding of meaningful work for further research and applied implications in work and organizational studies