98 research outputs found
Hydrokinetic predictions for femtoscopy scales in A+A collisions in the light of recent ALICE LHC results
A study of energy behavior of the pion spectra and interferometry scales is
carried out for the top SPS, RHIC and for LHC energies within the hydrokinetic
approach. The main mechanisms that lead to the paradoxical, at first sight,
dependence of the interferometry scales with an energy growth, in particular, a
decrease ratio, are exposed. The hydrokinetic predictions
for the HBT radii at LHC energies are compared with the recent results of the
ALICE experiment.Comment: Based on the talks given at the Sixth Workshop on Particle
Correlations and Femtoscopy, BITP, Kiev, September 14 - 18, 2010 and GSI/EMMI
Seminar, January 14, 201
Fast hadron freeze-out generator, part II: noncentral collisions
The fast Monte Carlo procedure of hadron generation developed in our previous
work is extended to describe noncentral collisions of nuclei. We consider
different possibilities to introduce appropriate asymmetry of the freeze-out
hyper-surface and flow velocity profile. For comparison with other models and
experimental data we demonstrate the results based on the standard
parametrizations of the hadron freeze-out hyper-surface and flow velocity
profile assuming either a common chemical and thermal freeze-out or the
chemically frozen evolution from chemical to thermal freeze-out. The C++
generator code is written under the ROOT framework and is available for public
use at http://uhkm.jinr.ru/Comment: 27 pages including 7 figures as EPS-files; prepared using LaTeX
package for publication in Physical Review
Antimicrobial Properties of Ethylene Vinyl Alcohol/Epsilon-Polylysine Films and Their Application in Surimi Preservation
[EN] Polymer films based on ethylene vinyl copolymers (EVOH) containing a 29 % (EVOH 29) and a 44 % molar percentage of ethylene (EVOH 44), and incorporating epsilon-polylysine (EPL) at 0 %, 1 %, 5 % and 10 % were successfully made by casting. The optical properties and the amount of EPL released from the films to phosphate buffer at pH 7.5 were evaluated, films showing great transparency and those of EVOH 29 copolymer releasing a greater amount of EPL. The antimicrobial properties of the resulting films were tested in vitro against different foodborne microorganisms and in vivo in surimi sticks. With regard to the antimicrobial capacity tested in vitro in liquid medium at 37 A degrees C and 4 A degrees C against Listeria monocytogenes and Escherichia coli over a period of 72 h, films showed a considerable growth inhibitory effect against both pathogens, more notably against L. monocytogenes, and being EVOH 29 more effective than EVOH 44 films. At 37 A degrees C, total growth inhibition was observed for EVOH 29 films incorporating 10 % EPL against both microorganisms whereas the copolymer EVOH 44 did show total inhibition against L. monocytogenes and the growth of E. coli was reduced by 6.64 log units. At 4 A degrees C, no film was able to inhibit completely bacterial growth. Scanning electron microscopy micrographs showed corrugated cell surfaces with blisters and bubbles, and collapse of the cells appearing shorter and more compact after treatment with EPL. Finally, the films were successfully used to increase the shelf life of surimi sticks. The results show the films developed have a great potential for active food packaging applications.The authors acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness, projects AGL2012-39920-C03-01, and fellowship funding for V. M.-G.Muriel-Galet, V.; Lopez-Carballo, G.; Gavara Clemente, R.; Hernández-Muñoz, P. (2014). Antimicrobial Properties of Ethylene Vinyl Alcohol/Epsilon-Polylysine Films and Their Application in Surimi Preservation. Food and Bioprocess Technology. 7(12):3548-3559. https://doi.org/10.1007/s11947-014-1363-1S35483559712Adams, M. R., & Moss, M. O. (2008). Food microbiology. UK: The Royal Society of Chemistry Cambridge.Aucejo, S., Catala, R., & Gavara, R. (2000). Interactions between water and EVOH food packaging films. Food Science and Technology International, 6(2), 159–164.Brandt, A. L., Castillo, A., Harris, K. B., Keeton, J. T., Hardin, M. D., & Taylor, T. M. (2010). Inhibition of Listeria monocytogenes by food antimicrobials applied singly and in combination. Journal of Food Science, 75(9), 557–563.Buchanan, R. L., & Doyle, M. P. (1997). Foodborne disease significance of Escherichia coli O157:H7 and other enterohemorrhagic E-coli. Food Technology, 51(10), 69–76.Chang, S.-S., Lu, W.-Y. W., Park, S.-H., & Kang, D.-H. (2010). Control of foodborne pathogens on ready-to-eat roast beef slurry by epsilon-polylysine. International Journal of Food Microbiology, 141(3), 236–241.Chang, Y., McLandsborough, L., & McClements, D. J. (2012). Cationic antimicrobial (epsilon-polylysine)-anionic polysaccharide (Pectin) interactions: influence of polymer charge on physical stability and antimicrobial efficacy. Journal of Agricultural and Food Chemistry, 60(7), 1837–1844.Chi-Zhang, Y. D., Yam, K. L., & Chikindas, M. L. (2004). Effective control of Listeria monocytogenes by combination of nisin formulated and slowly released into a broth system. International Journal of Food Microbiology, 90(1), 15–22.Coton, M., Denis, C., Cadot, P., & Coton, E. (2011). Biodiversity and characterization of aerobic spore-forming bacteria in surimi seafood products. Food Microbiology, 28(2), 252–260.FAO (2005) Further processing of fish Fisheries and Aquaculture Department, Rome. Updated 27 May 2005 Retrieved 14 March 2011.FDA (2004) Agency reponse letter GRAS Notice No. GRN 00135.Gambarin, P., Magnabosco, C., Losio, M. N., Pavoni, E., Gattuso, A., Arcangeli, G., et al. (2012). Listeria monocytogenes in ready-to-rat seafood and potential hazards for the consumers. International Journal of Microbiology, 2012, 497–635.Geornaras I, Yoon Y., Belk K. E., Smith G. C., Sofos J. N. (2007). Antimicrobial activity of epsilonpolylysine against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes in various food extracts. Journal of Food Science, 72(8), M330–4.Gunlu, A., & Koyun, E. (2013). Effects of vacuum packaging and wrapping with chitosan-based edible film on the extension of the shelf life of sea bass (Dicentrarchus labrax) fillets in cold storage (4 A degrees C). Food and Bioprocess Technology, 6(7), 1713–1719.Hiraki, J. (1995). Basic and applied studies on ε-polylysine. Journal of Antibacterial Antifungal Agents Japan, 23, 349–493.Hiraki, J. (2000). ε-Polylysine, its development and utilization. Fine Chemistry, 29, 18–25.Hiraki, J., Ichikawa, T., Ninomiya, S., Seki, H., Uohama, K., Kimura, S., et al. (2003). Use of ADME studies to confirm the safety of epsilon-polylysine as a preservative in food. Regulatory Toxicology and Pharmacology, 37(2), 328–340.Ho, Y. T., Ishizaki, S., & Tanaka, M. (2000). Improving emulsifying activity of epsilon-polylysine by conjugation with dextran through the Maillard reaction. Food Chemistry, 68(4), 449–455.Huss, H. H., Jorgensen, L. V., & Vogel, B. F. (2000). Control options for Listeria monocytogenes in seafoods. International Journal of Food Microbiology, 62(3), 267–274.Kaneko, K., Hayashidani, H., Ohtomo, Y., Kosuge, J., Kato, M., Takahashi, K., et al. (1999). Bacterial contamination of ready-to-eat foods and fresh products in retail shops and food factories. Journal of Food Protection, 62(6), 644–649.Kang, E. T., Tan, K. L., Kato, K., Uyama, Y., & Ikada, Y. (1996). Surface modification and functionalization of polytetrafluoroethylene films. Macromolecules, 29(21), 6872–6879.Li, J., Han, Q., Chen, W., & Ye, L. (2012). Antimicrobial activity of Chinese bayberry extract for the preservation of surimi. Journal of the Science of Food and Agriculture, 92(11), 2358–2365.Lopez de Dicastillo, C., Nerin, C., Alfaro, P., Catala, R., Gavara, R., & Hernandez-Munoz, P. (2011). Development of new antioxidant active packaging films based on ethylene vinyl alcohol copolymer (EVOH) and green tea extract. Journal of Agricultural and Food Chemistry, 59(14), 7832–7840.Lopez-de-Dicastillo, C., Alonso, J. M., Catala, R., Gavara, R., & Hernandez-Munoz, P. (2010). Improving the antioxidant protection of packaged food by incorporating natural flavonoids into ethylene-vinyl alcohol copolymer (EVOH) dilms. Journal of Agricultural and Food Chemistry, 58(20), 10958–10964.Lopez-de-Dicastillo, C., Pezo, D., Nerin, C., Lopez-Carballo, G., Catala, R., Gavara, R., et al. (2012). Reducing oxidation of foods through antioxidant active packaging based on ethyl vinyl alcohol and natural flavonoids. Packaging Technology and Science, 25(8), 457–466.M100-S22 (2012) Performance Standards for Antimicrobial Susceptibility Testing: Eighteenth Informational Supplement. Clinical and Laboratory Standards Institute. Advancing Quality in Health Care Testing. Vol. 32 No. 3. Replaces M100-S21 . Vol. 31 No. 1Mead, P. S., & Griffin, P. M. (1998). Escherichia coli O157:H7. Lancet, 352(9135), 1207–1212.Miya, S., Takahashi, H., Ishikawa, T., Fujii, T., & Kimura, B. (2010). Risk of Listeria monocytogenes xontamination of raw ready-to-eat seafood products available at retail outlets in Japan. Applied and Environmental Microbiology, 76(10), 3383–3386.Muriel-Galet, V., Cerisuelo, J. P., Lopez-Carballo, G., Lara, M., Gavara, R., & Hernandez-Munoz, P. (2012a). Development of antimicrobial films for microbiological control of packaged salad. International Journal of Food Microbiology, 157(2), 195–201.Muriel-Galet, V., Lopez-Carballo, G., Gavara, R., & Hernandez-Munoz, P. (2012b). Antimicrobial food packaging film based on the release of LAE from EVOH. International Journal of Food Microbiology, 157(2), 239–244.Muriel-Galet, V., Cerisuelo, J. P., Lopez-Carballo, G., Aucejo, S., Gavara, R., & Hernandez-Munoz, P. (2013a). Evaluation of EVOH-coated PP films with oregano essential oil and citral to improve the shelf-life of packaged salad. Food Control, 30(1), 137–143.Muriel-Galet, V., López-Carballo, G., Hernández-Muñoz, P., & Gavara, R. (2013b). Characterization of ethylene–vinyl alcohol copolymer containing lauril arginate (LAE) as material for active antimicrobial food packaging. Food Packaging and Shelf Life, 1, 10–17.Park, J. W. (2014). Surimi and surimi seafood. Boca Raton: CRC Press.Shima, S., & Sakai, H. (1977). Polylysine produced by Streptomyces. Agricultural and Biological Chemistry, 41(9), 1807–1809.Shima, S., Matsuoka, H., Iwamoto, T., & Sakai, H. (1984). Antimicrobial action of epsilon-poly-l-lysine. Journal of Antibiotics, 37(11), 1449–1455.Singh, R. K., & Balange, A. K. (2005). Characteristics of pink perch (Nemipterus japonicus) surimi at frozen temperature. Journal of Food Processing and Preservation, 29(1), 75–83.Suppakul, P., Miltz, J., Sonneveld, K., & Bigger, S. W. (2003). Active packaging technologies with an emphasis on antimicrobial packaging and its applications. Journal of Food Science, 68(2), 408–420.Ting, H. Y., Ishizaki, S., & Tanaka, M. (1999). Epsilon-polylysine improves the quality of surimi products. Journal of Muscle Foods, 10(4), 279–294.Tzschoppe, M., Martin, A., & Beutin, L. (2012). A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables. International Journal of Food Microbiology, 152(1–2), 19–30.Uchida, E., Uyama, Y., & Ikada, Y. (1993). Sorption of low-molecular-weight anions into thin polycation layers grafted onto a film. Langmuir, 9(4), 1121–1124.Unalan, I. U., Ucar, K. D. A., Arcan, I., Korel, F., & Yemenicioglu, A. (2011). Antimicrobial potential of polylysine in edible films. Food Science and Technology Research, 17(4), 375–380.Venugopal, V., & Shahidi, F. (1995). Value-added products from underutilized fish species. Critical Reviews in Food Science and Nutrition, 35(5), 431–453.Zambuchini, B., Fiorini, D., Verdenelli, M. C., Orpianesi, C., & Ballini, R. (2008). Inhibition of microbiological activity during sole (Solea solea L.) chilled storage by applying ellagic and ascorbic acids. LWT--Food Science and Technology, 41(9), 1733–1738.Zinoviadou, K. G., Koutsoumanis, K. P., & Biliaderis, C. G. (2010). Physical and thermo-mechanical properties of whey protein isolate films containing antimicrobials, and their effect against spoilage flora of fresh beef. Food Hydrocolloids, 24(1), 49–59
Cardiovascular risk assessment - From individual risk prediction to estimation of global risk and change in risk in the population
Cardiovascular disease is the most common cause of death and risk prediction formulae such as the Framingham Risk Score have been developed to easily identify patients at high risk that may require therapeutic interventions. Using cardiovascular risk formulae at a population level to estimate and compare average cardiovascular risk among groups has been recently proposed as a way to facilitate surveillance of net cardiovascular risk and target public health interventions. Risk prediction formulas may help to compare interventions that cause effects of different magnitudes and directions in several cardiovascular risk factors, because these formulas assess the net change in risk using easily obtainable clinical variables. Because of conflicting data estimates of the incidence and prevalence of cardiovascular disease, risk prediction formulae may be a useful tool to estimate such risk at a population level
Structural basis for DNA damage-induced phosphoregulation of MDM2 RING domain
Phosphorylation of MDM2 by ATM upon DNA damage is an important mechanism for deregulating MDM2, thereby leading to p53 activation. ATM phosphorylates multiple residues near the RING domain of MDM2, but the underlying molecular basis for deregulation remains elusive. Here we show that Ser429 phosphorylation selectively enhances the ubiquitin ligase activity of MDM2 homodimer but not MDM2-MDMX heterodimer. A crystal structure of phospho-Ser429 (pS429)-MDM2 bound to E2–ubiquitin reveals a unique 310-helical feature present in MDM2 homodimer that allows pS429 to stabilize the closed E2–ubiquitin conformation and thereby enhancing ubiquitin transfer. In cells Ser429 phosphorylation increases MDM2 autoubiquitination and degradation upon DNA damage, whereas S429A substitution protects MDM2 from auto-degradation. Our results demonstrate that Ser429 phosphorylation serves as a switch to boost the activity of MDM2 homodimer and promote its self-destruction to enable rapid p53 stabilization and resolve a long-standing controversy surrounding MDM2 auto-degradation in response to DNA damage
Longitudinal Evaluation of an N-Ethyl-N-Nitrosourea-Created Murine Model with Normal Pressure Hydrocephalus
Normal-pressure hydrocephalus (NPH) is a neurodegenerative disorder that usually occurs late in adult life. Clinically, the cardinal features include gait disturbances, urinary incontinence, and cognitive decline.Herein we report the characterization of a novel mouse model of NPH (designated p23-ST1), created by N-ethyl-N-nitrosourea (ENU)-induced mutagenesis. The ventricular size in the brain was measured by 3-dimensional micro-magnetic resonance imaging (3D-MRI) and was found to be enlarged. Intracranial pressure was measured and was found to fall within a normal range. A histological assessment and tracer flow study revealed that the cerebral spinal fluid (CSF) pathway of p23-ST1 mice was normal without obstruction. Motor functions were assessed using a rotarod apparatus and a CatWalk gait automatic analyzer. Mutant mice showed poor rotarod performance and gait disturbances. Cognitive function was evaluated using auditory fear-conditioned responses with the mutant displaying both short- and long-term memory deficits. With an increase in urination frequency and volume, the mutant showed features of incontinence. Nissl substance staining and cell-type-specific markers were used to examine the brain pathology. These studies revealed concurrent glial activation and neuronal loss in the periventricular regions of mutant animals. In particular, chronically activated microglia were found in septal areas at a relatively young age, implying that microglial activation might contribute to the pathogenesis of NPH. These defects were transmitted in an autosomal dominant mode with reduced penetrance. Using a whole-genome scan employing 287 single-nucleotide polymorphic (SNP) markers and further refinement using six additional SNP markers and four microsatellite markers, the causative mutation was mapped to a 5.3-cM region on chromosome 4.Our results collectively demonstrate that the p23-ST1 mouse is a novel mouse model of human NPH. Clinical observations suggest that dysfunctions and alterations in the brains of patients with NPH might occur much earlier than the appearance of clinical signs. p23-ST1 mice provide a unique opportunity to characterize molecular changes and the pathogenic mechanism of NPH
Impact of clinical phenotypes on management and outcomes in European atrial fibrillation patients: a report from the ESC-EHRA EURObservational Research Programme in AF (EORP-AF) General Long-Term Registry
Background: Epidemiological studies in atrial fibrillation (AF) illustrate that clinical complexity increase the risk of major adverse outcomes. We aimed to describe European AF patients\u2019 clinical phenotypes and analyse the differential clinical course. Methods: We performed a hierarchical cluster analysis based on Ward\u2019s Method and Squared Euclidean Distance using 22 clinical binary variables, identifying the optimal number of clusters. We investigated differences in clinical management, use of healthcare resources and outcomes in a cohort of European AF patients from a Europe-wide observational registry. Results: A total of 9363 were available for this analysis. We identified three clusters: Cluster 1 (n = 3634; 38.8%) characterized by older patients and prevalent non-cardiac comorbidities; Cluster 2 (n = 2774; 29.6%) characterized by younger patients with low prevalence of comorbidities; Cluster 3 (n = 2955;31.6%) characterized by patients\u2019 prevalent cardiovascular risk factors/comorbidities. Over a mean follow-up of 22.5 months, Cluster 3 had the highest rate of cardiovascular events, all-cause death, and the composite outcome (combining the previous two) compared to Cluster 1 and Cluster 2 (all P <.001). An adjusted Cox regression showed that compared to Cluster 2, Cluster 3 (hazard ratio (HR) 2.87, 95% confidence interval (CI) 2.27\u20133.62; HR 3.42, 95%CI 2.72\u20134.31; HR 2.79, 95%CI 2.32\u20133.35), and Cluster 1 (HR 1.88, 95%CI 1.48\u20132.38; HR 2.50, 95%CI 1.98\u20133.15; HR 2.09, 95%CI 1.74\u20132.51) reported a higher risk for the three outcomes respectively. Conclusions: In European AF patients, three main clusters were identified, differentiated by differential presence of comorbidities. Both non-cardiac and cardiac comorbidities clusters were found to be associated with an increased risk of major adverse outcomes
Pan-cancer analysis of whole genomes
Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale(1-3). Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4-5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter(4); identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation(5,6); analyses timings and patterns of tumour evolution(7); describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity(8,9); and evaluates a range of more-specialized features of cancer genomes(8,10-18).Peer reviewe
Brucellosis as an Emerging Threat in Developing Economies:Lessons from Nigeria
Nigeria is the most populous country in Africa, has a large proportion of the world's poor livestock keepers, and is a hotspot for neglected zoonoses. A review of the 127 accessible publications on brucellosis in Nigeria reveals only scant and fragmented evidence on its spatial and temporal distribution in different epidemiological contexts. The few bacteriological studies conducted demonstrate the existence of Brucella abortus in cattle and sheep, but evidence for B. melitensis in small ruminants is dated and unclear. The bulk of the evidence consists of seroprevalence studies, but test standardization and validation are not always adequately described, and misinterpretations exist with regard to sensitivity and/or specificity and ability to identify the infecting Brucella species. Despite this, early studies suggest that although brucellosis was endemic in extensive nomadic systems, seroprevalence was low, and brucellosis was not perceived as a real burden; recent studies, however, may reflect a changing trend. Concerning human brucellosis, no studies have identified the Brucella species and most reports provide only serological evidence of contact with Brucella in the classical risk groups; some suggest brucellosis misdiagnoses as malaria or other febrile conditions. The investigation of a severe outbreak that occurred in the late 1970s describes the emergence of animal and human disease caused by the settling of previously nomadic populations during the Sahelian drought. There appears to be an increasing risk of re-emergence of brucellosis in sub-Saharan Africa, as a result of the co-existence of pastoralist movements and the increase of intensive management resulting from growing urbanization and food demand. Highly contagious zoonoses like brucellosis pose a threat with far-reaching social and political consequences
A Review of the Efficacy and Tolerability of Combination Amlodipine/Valsartan in Non-White Patients with Hypertension
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