Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

The positive role of fatty acids in the prevention and alleviation of non-human and human diseases have been and continue to be extensively documented. These roles include influences on infectious and non-infectious diseases including prevention of inflammation as well as mucosal immunity to infectious diseases. Cholera is an acute intestinal illness caused by the bacterium Vibrio cholerae. It occurs in developing nations and if left untreated, can result in death. While vaccines for cholera exist, they are not always effective and other preventative methods are needed. We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin using mouse BALB/C macrophages and human intestinal epithelial cells, respectively. We solubilized the above fatty acids and used cell proliferation assays to determine the concentration ranges and specific concentrations of the fatty acids that are not detrimental to human intestinal epithelial cell viability. We solubilized cholera toxin and used it in an assay to determine the concentration ranges and specific concentrations of cholera toxin that do not statistically decrease cell viability in BALB/C macrophages. We found the optimum fatty acid concentrations to be between 1-5 ng/μl, and that for cholera toxin to be < 30 ng per treatment. This data may aid future studies that aim to find a protective mucosal role for fatty acids in prevention or alleviation of cholera infections

Human–Canine Interaction: Exploring Stress Indicator Response Patterns of Salivary Cortisol and Immunoglobulin A

A repeated measures design was used to analyze the effect of a canine interaction on salivary cortisol and immunoglobulin A (IgA) in 33 adults; 16 were pet owners and 17 were non-pet owners. Cortisol and IgA levels before and after a canine interaction (experimental) or viewing a canine movie (control) were measured by enzyme-linked immunosorbent assay and spectrophotometer. Data show a significant interaction effect for salivary cortisol in non-pet owners (p 5 0.003). Changes in IgA levels were not significant. The findings suggested that interaction with canines may help reduce the biological effects of stress that influences human health. Further studies with larger sample sizes are necessary to support these results. © 2012 Springer Publishing Company

Human-canine interaction: Exploring stress indicator response patterns of salivary cortisol and immunoglobulin A

A repeated measures design was used to analyze the effect of a canine interaction on salivary cortisol and immunoglobulin A (IgA) in 33 adults; 16 were pet owners and 17 were non-pet owners. Cortisol and IgA levels before and after a canine interaction (experimental) or viewing a canine movie (control) were measured by enzyme-linked immunosorbent assay and spectrophotometer. Data show a significant interaction effect for salivary cortisol in non-pet owners (p 5 0.003). Changes in IgA levels were not significant. The findings suggested that interaction with canines may help reduce the biological effects of stress that influences human health. Further studies with larger sample sizes are necessary to support these results. © 2012 Springer Publishing Company