Enterohaemorrhagic Escherichia coli and Shigella dysenteriae type 1-induced haemolytic uraemic syndrome

Haemolytic uraemic syndrome (HUS) can be classified according to the aetiology of the different disorders from which it is composed. The most prevalent form is that induced by shigatoxin producing Escherichia coli (STEC) and, in some tropical regions, by Shigella dysenteriae type 1. STEC cause a zoonosis, are widely distributed in nature, enter the food chain in different ways, and show regional differences. Not all STEC are human pathogens. Enterohaemorrhagic E. coli usually cause attachment and effacing lesions in the intestine. This is not essential, but production of a shigatoxin (Stx) is. Because Stx are encoded by a bacteriophage, this property is transferable to naïve strains. Laboratory methods have improved by identifying STEC either via the toxin or its bacteriophage. Shigella dysenteriae type 1 produces shigatoxin, identical to Stx-1, but also has entero-invasive properties that enterohaemorrhagic Escherichia coli (EHEC) do not. Shigella patients risk bacteremia and benefit from early antibiotic treatment, unlike those with EHEC

Narrative exposure therapy for PTSD increases top-down processing of aversive stimuli - evidence from a randomized controlled treatment trial

Adenauer H, Catani C, Gola H, et al. Narrative exposure therapy for PTSD increases top-down processing of aversive stimuli - evidence from a randomized controlled treatment trial. BMC Neuroscience. 2011;12(1): 127.BACKGROUND: Little is known about the neurobiological foundations of psychotherapy for Posttraumatic Stress Disorder (PTSD). Prior studies have shown that PTSD is associated with altered processing of threatening and aversive stimuli. It remains unclear whether this functional abnormality can be changed by psychotherapy. This is the first randomized controlled treatment trial that examines whether narrative exposure therapy (NET) causes changes in affective stimulus processing in patients with chronic PTSD. METHODS: 34 refugees with PTSD were randomly assigned to a NET group or to a waitlist control (WLC) group. At pre-test and at four-months follow-up, the diagnostics included the assessment of clinical variables and measurements of neuromagnetic oscillatory brain activity (steady-state visual evoked fields, ssVEF) resulting from exposure to aversive pictures compared to neutral pictures. RESULTS: PTSD as well as depressive symptom severity scores declined in the NET group, whereas symptoms persisted in the WLC group. Only in the NET group, parietal and occipital activity towards threatening pictures increased significantly after therapy. CONCLUSIONS: Our results indicate that NET causes an increase of activity associated with cortical top-down regulation of attention towards aversive pictures. The increase of attention allocation to potential threat cues might allow treated patients to re-appraise the actual danger of the current situation and, thereby, reducing PTSD symptoms. REGISTRATION OF THE CLINICAL TRIAL: Number: NCT00563888Name: "Change of Neural Network Indicators Through Narrative Treatment of PTSD in Torture Victims" ULR: http://www.clinicaltrials.gov/ct2/show/NCT00563888

Pan-cancer analysis of whole genomes

Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale(1-3). Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4-5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter(4); identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation(5,6); analyses timings and patterns of tumour evolution(7); describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity(8,9); and evaluates a range of more-specialized features of cancer genomes(8,10-18).Peer reviewe

Expert based model building to quantify risk factors in a combined aquaculture-agriculture system

In recent years, across tropical regions of the world, there has been an expansion of integrated farming systems that combine rice and shrimp production. While these systems were developed as a form of crop-rotation – growing rice in the wet season and shrimp in the dry season – some farmers grow both rice and brackish-water shrimp simultaneously during the wet season. Climatic variability has resulted in considerable crop losses in this system across many regions. Research has yet to identify the complete array of key risk factors, and their potential interactions, for integrated rice-shrimp farming. Consequently, different farming practices and environmental factors that may affect crop production need to be clarified to guide research efforts. We applied a staged, iterative process to develop a probabilistic Bayesian belief network based on expert knowledge that describes the relationships that contribute to the risk of failure of both crops in integrated rice-shrimp farming systems during the wet season. We applied the approach in the Southern Mekong Delta, Vietnam, in the context of a broader research program into the sustainability of the rice-shrimp farming system. The resulting network represents the experts' perceptions of the key risk factors to production and the interactions among them. While both farmers and extension officers contributed to the identification of the processes included in the network, the farmers alone provided estimates of the probability of the relationships among them. The network identified the challenges to minimise the risk of failure for both crops, and the steps farmers can take to mitigate some of them. Overall, farmers perceived they have a better chance to minimise risk of failure for shrimp rather than rice crops, and limited opportunities appear to exist for successful production of both. By engaging the farmers in this process of model development, we were able to identify additional research questions for the broader research team and to identify simple steps the farmers could take to reduce the risk of crop failure. Integrating additional empirical data into this network, as it becomes available, will help identify clear opportunities for improvements in farming practices which should reduce the risk of crop failure into the future

Land–atmosphere feedbacks amplify aridity increase over land under global warming

International audienc

βdecay of 61 Mn to levels in 61 Fe

A detailed β-decay study of 61Mn is presented, yielding extended information on the level structure of 61Fe. Pure beams were obtained at ISOLDE, CERN, after selective laser ionization and mass separation of fission products from the bombardment of a UCx target by 1.4-GeV protons. The β and γ information was detected by two MiniBall clusters and three Eplastic scintillators. The new 61Mn decay scheme reveals 48γtransitions, distributed over 20 excited states. A comparison to the decay scheme of 59Mn and excited states in 59Fe is made. Shell-model calculations with two different interactions are performed in order to compare the nuclear structure of the two neighboring odd-Airon isotopes. Tentative spin and parities of several excited states in 61Fe are assigned on the basis of β-decay feeding patterns in both 59,61Fe and of results from the theoretical shell-model calculations.status: publishe

β − decay of the neutron-rich isotope 215Pb

This Brief Report reports on the first observation of the β− delayed γ decay of 215Pb, feeding states in 215Bi. The 215Pb beam was produced using resonant laser ionization and mass separated at the ISOLDE-CERN on-line mass separator. This ensured clean identification of theγrays as belonging to the decay of 215Pb or its β-decay daughters. A half-life of 147(12) s was measured for the 215Pb β decay and a level scheme for the daughter nucleus 215Bi is proposed, resulting in an extended systematics of the excited states of the neutron-rich Bi isotopes.status: publishe