Salivary flow rate, pH, and concentrations of calcium, phosphate, and sIgA in Brazilian pregnant and non-pregnant women

BACKGROUND: Studies on salivary variables and pregnancy in Latin America are scarce. This study aimed to compare salivary flow rate, pH, and concentrations of calcium, phosphate, and sIgA of unstimulated whole saliva in pregnant and non-pregnant Brazilians. METHODS: Cross-sectional study. Sample was composed by 22 pregnant and 22 non-pregnant women attending the Obstetrics and Gynecology Clinics, São Lucas Hospital, in Porto Alegre city, South region of Brazil. Unstimulated whole saliva was collected to determine salivary flow rate, pH, and biochemical composition. Data were analyzed by Student t test and ANCOVA (two-tailed α = 0.05). RESULTS: No difference was found for salivary flow rates and concentrations of total calcium and phosphate between pregnant and non-pregnant women (p > 0.05). Pregnant women had lower pH (6.7) than non-pregnant women (7.5) (p < 0.001), but higher sIgA level (118.9 mg/L) than the latter (90.1 mg/L) (p = 0.026). CONCLUSION: Some of the tested variables of unstimulated whole saliva were different between pregnant and non-pregnant Brazilians in this sample. Overall, the values of the tested salivary parameters were within the range of international references of normality

Pan-cancer analysis of whole genomes

Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale(1-3). Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4-5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter(4); identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation(5,6); analyses timings and patterns of tumour evolution(7); describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity(8,9); and evaluates a range of more-specialized features of cancer genomes(8,10-18).Peer reviewe

Scales and structure of frontal adjustment and freshwater export in a region of freshwater influence

Sea surface temperature satellite imagery and a regional hydrodynamic model are used to investigate the variability and structure of the Liverpool Bay thermohaline front. A statistically based water mass classification technique is used to locate the front in both data sets. The front moves between 5 and 35 km in response to spring-neap changes in tidal mixing, an adjustment that is much greater than at other shelf-sea fronts. Superimposed on top of this fortnightly cycle are semi-diurnal movements of 5-10 km driven by flood and ebb tidal currents. Seasonal variability in the freshwater discharge and the density difference between buoyant inflow and more saline Irish Sea water give rise to two different dynamical regimes. During winter, when cold inflow reduces the buoyancy of the plume, a bottom-advected front develops. Over the summer, when warm river water provides additional buoyancy, a surface-advected plume detaches from the bottom and propagates much larger distances across the bay. Decoupled from near-bed processes, the position of the surface front is more variable. Fortnightly stratification and re-mixing over large areas of Liverpool Bay is a potentially important mechanism by which freshwater, and its nutrient and pollutant loads, are exported from the coastal plume system. Based on length scales estimated from model and satellite data, the erosion of post-neap stratification is estimated to be responsible for exporting approximately 19% of the fresh estuarine discharge annually entering the system. Although the baroclinic residual circulation makes a more significant contribution to freshwater fluxes, the episodic nature of the spring-neap cycle may have important implications for biogeochemical cycles within the ba