763 research outputs found
Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved waterâuse efficiency (WUE) and drought resilience in C3 plants while sustaining productivity in the hotter and drier climates that are predicted for much of the world. CAM species show an inverted pattern of stomatal opening and closing across the diel cycle, which conserves water and provides a means of maintaining growth in hot, waterâlimited environments. Recent genome sequencing of the constitutive model CAM species KalanchoĂ« fedtschenkoi provides a platform for elucidating the ensemble of proteins that link photosynthetic metabolism with stomatal movement, and that protect CAM plants from harsh environmental conditions. We describe a largeâscale proteomics analysis to characterize and compare proteins, as well as diel changes in their abundance in guard cellâenriched epidermis and mesophyll cells from leaves of K. fedtschenkoi. Proteins implicated in processes that encompass respiration, the transport of water and CO2, stomatal regulation, and CAM biochemistry are highlighted and discussed. Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared with that observed in Arabidopsis is reported and tissueâspecific localization in the epidermis and mesophyll of isozymes implicated in starch and malate turnover are discussed in line with the contrasting roles for these metabolites within the CAM mesophyll and stomatal complex. These data reveal the proteins and the biological processes enriched in each layer and provide key information for studies aiming to adapt plants to hot and dry environments by modifying leaf physiology for improved plant sustainability
The effect of biomass ashes and potassium salts on MEA degradation for BECCS
This study investigates the comparative impact of inherently different biomass and coal ashes on the laboratory and pilot scale degradation of 30 wt% aqueous monoethanolamine (MEA), relevant to post-combustion CO2 capture. Thermal and oxidative degradation experiments were carried out at 135 °C and 40 °C respectively with CO2 loading (0.5 molCO2/molMEA), with and without the presence of ash. Nuclear magnetic resonance (NMR) data is provided for the major MEA degradation compounds such as N-(2-hydroxyethyl)formamide (HEF) and N-(2-hydroxyethyl)imidazole (HEI) along with the characterisation of a new MEA oxidative degradation product, N-(2-hydroxyethyl)imidazole-N-oxide (HEINO) which had been previously misassigned. Degradation products were quantified using 1H NMR and gas chromatography mass spectrometry (GCâMS) to assess the impact of potassium and various ashes from combustion (olive, white wood and two types of coal ash) on the rates of amine degradation. Woody biomass fly ashes were found to reduce the presence of the oxidative degradation products. Both types of coal fly ash and the olive biomass ash were found to enhance the formation the newly identified degradation product, HEINO. Solvent samples taken from a pilot scale facility support these laboratory findings
Symptom burden according to dialysis day of the week in three times a week haemodialysis patients
Background
Haemodialysis patients experience significant symptom burden and effects on healthrelated quality of life. Studies have shown increases in fluid overload, hospitalization and
mortality immediately after the long interdialytic interval in thrice weekly in-centre haemodialysis patients, however the relationship between the dialytic interval and patient reported
outcome measures (PROMs) has not been quantified and the extent to which dialysis day of
PROM completion needs to be standardised is unknown.
Methods
Three times a week haemodialysis patients participating in a stepped wedge trial to increase
patient participation in haemodialysis tasks completed PROMs (POS-S Renal symptom
score and EQ-5D-5L) at recruitment, six, 12 and 18 months. Time from the long interdialytic
interval, HD day of the week, and HD days vs non-HD days were included in mixed effects
Linear Regression, estimating severity (none to overwhelming treated as 0 to 4) of 17 symptoms and EQ-5D-5L, adjusting for age, sex, time on HD, control versus intervention and
Charlson Comorbidity Score.
Results
517 patients completed 1659 YHS questionnaires that could be assigned HD day (510 on
Mon/Tue/Sun, 549 on Wed/Thu/Tue, 308 on Fri/Sat/Thu and 269 on non-HD days). With
the exception of restless legs and skin changes, there was no statistically significant change
in symptom severity or EQ-5D-5L with increasing time from the long interdialytic interval.
Patients who responded on non-HD days had higher severity of poor appetite, constipation,
difficulty sleeping, poor mobility and depression (approximately 0.2 severity level), and
lower EQ-5D-5L (-0.06, CI -0.09 to -0.03) compared to HD days.
PLOS ONE
PLOS ONE | https://doi.org/10.1371/journal.pone.0274599 September 27, 2022 1 / 13
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OPEN ACCESS
Citation: Hnynn Si PE, Gair R, Barnes T, Dunn L,
Lee S, Ariss S, et al. (2022) Symptom burden
according to dialysis day of the week in three times
a week haemodialysis patients. PLoS ONE 17(9):
e0274599. https://doi.org/10.1371/journal.
pone.0274599
Editor: Gianpaolo Reboldi, Universita degli Studi di
Perugia, ITALY
Received: August 8, 2021
Accepted: August 31, 2022
Published: September 27, 2022
Peer Review History: PLOS recognizes the
benefits of transparency in the peer review
process; therefore, we enable the publication of
all of the content of peer review and author
responses alongside final, published articles. The
editorial history of this article is available here:
https://doi.org/10.1371/journal.pone.0274599
Copyright: © 2022 Hnynn Si et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.
Data Availability Statement: A minimal dataset
required to reach the conclusions drawn from this
manuscript required the linkage of identifiable
patient information collected during the trial to
Conclusions
Measuring symptom severity and EQ-5D-5L in haemodialysis populations does not need to
account for dialysis schedule, but completion either on HD or non-HD days could introduce
bias that may impact evaluation of interventions. Researchers should ensure completion of
these instruments are standardized on either dialysis or non-dialysis days
Forgiveness and the Need to Belong
People who experience a strong need to belong might be particularly inclined to forgive wrongdoings to preserve social bonds. Three studies that utilized different methods and measures of forgiveness consistently demonstrated this is not the case. The authors found that individuals high in the need to belong report practicing forgiveness with less frequency and value it no more than those low in the need to belong (Study 1). In Study 2, they found that satisfying the need to belong led participants to express greater willingness to forgive hypothetical offenses compared to participants in a control group. Finally, in Study 3, the authors linked the need to belong to forgiveness of specific transgressions and found that this negative relationship was mediated by offense-related anger and perceptions of offense severity. These findings suggest that needing to belong paradoxically interferes with forgiveness, even though forgiving could promote the satisfaction of belongingness needs following transgressions.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline
Shrinking a large dataset to identify variables associated with increased risk of Plasmodium falciparum infection in Western Kenya
Large datasets are often not amenable to analysis using traditional single-step approaches. Here, our general objective was to apply imputation techniques, principal component analysis (PCA), elastic net and generalized linear models to a large dataset in a systematic approach to extract the most meaningful predictors for a health outcome. We extracted predictors for Plasmodium falciparum infection, from a large covariate dataset while facing limited numbers of observations, using data from the People, Animals, and their Zoonoses (PAZ) project to demonstrate these techniques: data collected from 415 homesteads in western Kenya, contained over 1500 variables that describe the health, environment, and social factors of the humans, livestock, and the homesteads in which they reside. The wide, sparse dataset was simplified to 42 predictors of P. falciparum malaria infection and wealth rankings were produced for all homesteads. The 42 predictors make biological sense and are supported by previous studies. This systematic data-mining approach we used would make many large datasets more manageable and informative for decision-making processes and health policy prioritization
Global Search for New Physics with 2.0/fb at CDF
Data collected in Run II of the Fermilab Tevatron are searched for
indications of new electroweak-scale physics. Rather than focusing on
particular new physics scenarios, CDF data are analyzed for discrepancies with
the standard model prediction. A model-independent approach (Vista) considers
gross features of the data, and is sensitive to new large cross-section
physics. Further sensitivity to new physics is provided by two additional
algorithms: a Bump Hunter searches invariant mass distributions for "bumps"
that could indicate resonant production of new particles; and the Sleuth
procedure scans for data excesses at large summed transverse momentum. This
combined global search for new physics in 2.0/fb of ppbar collisions at
sqrt(s)=1.96 TeV reveals no indication of physics beyond the standard model.Comment: 8 pages, 7 figures. Final version which appeared in Physical Review D
Rapid Communication
Observation of Orbitally Excited B_s Mesons
We report the first observation of two narrow resonances consistent with
states of orbitally excited (L=1) B_s mesons using 1 fb^{-1} of ppbar
collisions at sqrt{s} = 1.96 TeV collected with the CDF II detector at the
Fermilab Tevatron. We use two-body decays into K^- and B^+ mesons reconstructed
as B^+ \to J/\psi K^+, J/\psi \to \mu^+ \mu^- or B^+ \to \bar{D}^0 \pi^+,
\bar{D}^0 \to K^+ \pi^-. We deduce the masses of the two states to be m(B_{s1})
= 5829.4 +- 0.7 MeV/c^2 and m(B_{s2}^*) = 5839.7 +- 0.7 MeV/c^2.Comment: Version accepted and published by Phys. Rev. Let
Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set
We report a measurement of the bottom-strange meson mixing phase \beta_s
using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays
in which the quark-flavor content of the bottom-strange meson is identified at
production. This measurement uses the full data set of proton-antiproton
collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment
at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity.
We report confidence regions in the two-dimensional space of \beta_s and the
B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2,
-1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in
agreement with the standard model expectation. Assuming the standard model
value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +-
0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +-
0.009 (syst) ps, which are consistent and competitive with determinations by
other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012
Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV
Results are presented from a search for a W' boson using a dataset
corresponding to 5.0 inverse femtobarns of integrated luminosity collected
during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV.
The W' boson is modeled as a heavy W boson, but different scenarios for the
couplings to fermions are considered, involving both left-handed and
right-handed chiral projections of the fermions, as well as an arbitrary
mixture of the two. The search is performed in the decay channel W' to t b,
leading to a final state signature with a single lepton (e, mu), missing
transverse energy, and jets, at least one of which is tagged as a b-jet. A W'
boson that couples to fermions with the same coupling constant as the W, but to
the right-handed rather than left-handed chiral projections, is excluded for
masses below 1.85 TeV at the 95% confidence level. For the first time using LHC
data, constraints on the W' gauge coupling for a set of left- and right-handed
coupling combinations have been placed. These results represent a significant
improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe
Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV
A search for a Higgs boson decaying into two photons is described. The
analysis is performed using a dataset recorded by the CMS experiment at the LHC
from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an
integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross
section of the standard model Higgs boson decaying to two photons. The expected
exclusion limit at 95% confidence level is between 1.4 and 2.4 times the
standard model cross section in the mass range between 110 and 150 GeV. The
analysis of the data excludes, at 95% confidence level, the standard model
Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The
largest excess of events above the expected standard model background is
observed for a Higgs boson mass hypothesis of 124 GeV with a local significance
of 3.1 sigma. The global significance of observing an excess with a local
significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is
estimated to be 1.8 sigma. More data are required to ascertain the origin of
this excess.Comment: Submitted to Physics Letters
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