Archivio Istituzionale della Ricerca- Università degli Studi di Foggia

    Probiotic characteristics in Saccharomyces cerevisiae strains: Properties for application in food industries

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    In this work, the probiotic or health-beneficial potential of 142 Saccharomyces strains isolated from food ecosystems belonging to the collection of the Yeast Biotechnology Laboratory (University of Castilla-La Mancha) have been studied. They were screened for their ability to resist and grow when exposed to simulated in vitro digestion conditions. As a result, 44 strains showed good kinetic properties and were chosen through k-means and one-way ANOVA and used to assess other important probiotic characteristics. For the second step, a Principal Component Analysis was run with the results from auto-aggregation and hydrophobicity at 30 °C assays, antibiotic resistance and antimicrobial activity, and 10 yeasts were then selected in order to study their biofilm formation capability, auto-aggregation and hydrophobicity at 37 °C, viability and biofilm formation after simulated sequential salivary-gastric-intestinal digestion. The most relevant results were those that showed the biofilm formation and hydrophobicity behaviour, so they were used for characterized the most promising probiotic candidates. The final results showed that the strains 132, 139 and 146 were the best ones

    Alginate-microencapsulation of Lactobacillus casei and Bifidobacterium bifidum: Performances of encapsulated microorganisms and bead-validation in lamb rennet

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    Lactobacillus casei Lc01 and Bifidobacterium bifidum Bb02 were loaded in 2%-alginate beads and stored at 4 °C for 1 month. After the storage, some technological and probiotic properties were measured on the cells released from beads. Free cells (microorganisms grown at 30–37 °C for 24 h and immediately used) and cells frozen with a cryoprotective agent (33% glycerol) and stored for 1 month were used as negative and positive controls, respectively. The loading of probiotics in beads did not affect acidification (ΔpH of 1.4–1.6), neither growth index at 15 (15–25%), 45 °C (34–45% for Bb02 and 57–67% for Lc01), at pH 9 (46–57%) or in presence of 3% NaCl (75–88%). Concerning the viability at pH 2.5 and in presence of bile salts, the cells released from beads behaved like free ones (viability loss of 2–3 log cfu/mL for both the strains), while frozen cells experienced a stronger cell decrease (5–6 log cfu/mL). As an additional goal, a validation of beads in a lamb rennet was proposed to design a new kind of rennet with “probiotics in” but protected from the harsh conditions. The strains were released from beads and survived for at least 47–49 days

    Le tutele welfaristiche nel Social Pillar

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    Angiopoietin-1 haploinsufficiency affects the endothelial barrier and causes hereditary angioedema

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    Background: Different mutations of the angiopoietin-1 gene (ANGPT1) have been associated with the occurrence of hereditary angioedema (HAE). Objective: The purpose of the study is to clarify whether the ANGPT1 A119S variant plays its role via haploinsufficiency or a dominant negative effect. Methods: The ability of ANGPT1 A119S variant to affect the endothelial barrier function was assessed by immunocytochemistry. Inter-endothelial gap formation molecules primarily responsible for cell-cell adhesions of HUVECs, vascular endothelial (VE)-cadherin and β-catenin, and reorganization of the F-actin cytoskeletal were evaluated. Results: In in vitro conditions mimicking the heterozygous state, the p.A119S variant significantly reduced the capability to bind its natural receptor (80.7% of normal), less than the homozygous condition (59.1%). After stimulation of VEGF or bradykinin, the addiction to equimolar amounts of wtANGPT1 and ANGPT1 p.A119S clearly reduced the expression of VE-cadherin on the endothelial cell surface (31% and 24% respectively). Likewise, cell surface expression of β-catenin was reduced and severe gap formation between adjacent HUVECs developed. In cultured cells, β-catenin expression was mostly observed along the cell surface. Treatment with equimolar amounts of wtANGPT1 and ANGPT1 p.A119S failed to restore the reorganization of the F-actin cytoskeletal elements. ANGPT1 p.A119S variant in homozygous condition further diminished VE-cadherin and β-catenin expression and failed to reduce stress fibre formation significantly affecting the endothelial barrier functionality. Conclusions and Clinical Relevance: Present data show that in a heterozygous state the p.A119S substitution results in a pathogenic loss of function of the protein due to a mechanism of haploinsufficiency. The ANGPT1 reduced ability to counteract the increment of endothelial permeability produced by inducers, such as VEGF and bradykinin, stimulate vascular leakage and reorganization of the F-actin cytoskeletal elements. As a result, a partial impairment of the ANGPT1 functionality, like when dominant mutations occur, represents a pathophysiological cause of HAE

    Impacts of climate change on the wine sector in Italy and mitigation and adaptation strategies.

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    The expected changes in climate are likely to influence the production of main crops. In Italy, one of the main producers of grapes and wine, the growing of vine is at risk. In particular, the climate changes are likely to influence the diffusion of diseases and pests, the use of water resources, and to influence the quantity and quality of productions. The impacts are heterogeneous across regions and call for specific risk management strategies. This chapter reviews the impacts of climate change in selected Italian regions, and the on-farm and off-farm risk management strategies that may help coping with the risks induced by climate changes. It is emphasized the role of crop insurance, which represents not only a mitigation strategy that helps reducing the negative economic consequences of extreme events, but also an adaptation strategy for complementing or substituting other risk management strategies. Es probable que los cambios previstos en el clima influyan en la producción de los principales cultivos. En Italia, uno de los principales países productores de uva y vino, el cultivo de la vid está en riesgo. En particular, es probable que los cambios climáticos influyan en la difusión de enfermedades y plagas y el uso de los recursos hídricos, y que influyan en la cantidad y calidad de las producciones. Los impactos son heterogéneos en todas las regiones y requieren estrategias de gestión de riesgos específicas. Este capítulo revisa los impactos del cambio climático en algunas regiones italianas seleccionadas, y las estrategias de gestión dentro y fuera de la finca que puede

    Alba Fucens, Forum (parte orientale)

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    A review of suicides by burning in Rome between 1947-1997 examined by the Pathology Department of the Institute of Forensic Medicine, University of Rome 'La Sapienza'

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    The 34 cases of suicide by self-immolation admitted to the Institute of Forensic Medicine, University of Rome 'La Sapienza', during a 50-year period (1947-1997) were investigated. The nature of this phenomenon in relation to sex, age of the victims, location of the suicides and combination of methods used is discussed. Finally the distribution and extent and characteristics of the lesions are considered from a forensic point of view. Copyright © 2001 Elsevier Science Ltd and ISBI
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