Mesonia sediminis sp nov., isolated from a sea cucumber culture pond

A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326(T), was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122A degrees 14'34aEuro(3)E 36A degrees 54'36aEuro(3)N). Cells of strain MF326(T) were found to be catalase negative and oxidase positive. Optimal growth was found to occur at 30 A degrees C and pH 7.0-7.5 in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MF326(T) is a member of the genus Mesonia and exhibits the high sequence similarity (94.3 %) with the type strain of Mesonia ostreae, followed by Mesonia algae (93.9 %). The dominant fatty acids of strain MF326(T) were identified as iso-C-15:0, an unidentified fatty acid with an equivalent chain-length of 13.565 and anteiso-C-15:0. The major polar lipids were found to be two unidentified lipids and phosphatidylethanolamine. The major respiratory quinone was found to be MK-6 and the genomic DNA G+C content was determined to be 40.7 mol%. On the basis of the phylogenetic analysis and differential phenotypic characteristics, it is concluded that strain MF326(T) (=KCTC 42255(T) =MCCC 1H00125(T)) should be assigned to the genus Mesonia as the type strain of a novel species, for which the name Mesonia sediminis sp. nov. is proposed.A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326(T), was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122A degrees 14'34aEuro(3)E 36A degrees 54'36aEuro(3)N). Cells of strain MF326(T) were found to be catalase negative and oxidase positive. Optimal growth was found to occur at 30 A degrees C and pH 7.0-7.5 in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MF326(T) is a member of the genus Mesonia and exhibits the high sequence similarity (94.3 %) with the type strain of Mesonia ostreae, followed by Mesonia algae (93.9 %). The dominant fatty acids of strain MF326(T) were identified as iso-C-15:0, an unidentified fatty acid with an equivalent chain-length of 13.565 and anteiso-C-15:0. The major polar lipids were found to be two unidentified lipids and phosphatidylethanolamine. The major respiratory quinone was found to be MK-6 and the genomic DNA G+C content was determined to be 40.7 mol%. On the basis of the phylogenetic analysis and differential phenotypic characteristics, it is concluded that strain MF326(T) (=KCTC 42255(T) =MCCC 1H00125(T)) should be assigned to the genus Mesonia as the type strain of a novel species, for which the name Mesonia sediminis sp. nov. is proposed

Pan-cancer analysis of whole genomes

Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale(1-3). Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4-5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter(4); identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation(5,6); analyses timings and patterns of tumour evolution(7); describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity(8,9); and evaluates a range of more-specialized features of cancer genomes(8,10-18).Peer reviewe

A 90.6% Efficient, 0.333 W/mm2 Power Density Direct 48V-to-1V Dual Inductor Hybrid Converter with Delay-line Based V2D Controller

This brief presents a 48V-to-1V 10-level dual inductor hybrid converter (DIHC) containing 11 on-chip switches and an off-chip gallium nitride (GaN) switch. Thanks to the 10-level Dickson switched-capacitor (SC) circuit, most of the voltage stress will be taken over by off-chip capacitors, which reduces the voltage stress of each switch to 4.8 V and takes full advantage of the voltage pressure on the 5-V on-chip transistors. This proposed structure is implemented in a 0.18- $\mu \text{m}$ BCD process to convert 48-V input to 1-V output with up to 18-A current load. The post-layout simulations show that a peak power efficiency of 90.6% can be achieved at 5.2-A loading and the power density is about 0.333 $W/mm^{2}$ considering the power stage area.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic Instrumentatio

Analysis of Settlement Space Environment along China’s Grand Canal Tianjin Section Based on Structural Equation Model—Case Study of 44 Typical Settlements

The settlement space along China’s Grand Canal is an important part of cultural heritage, boasting high value of conservation and inheritance as a museum of local culture in an active state. The paper is aimed at revealing the current situation and satisfaction with the settlement space environment along China’s Grand Canal Tianjin Section from the perspective of resident perception. We set up a structural equation model to perform an empirical analysis of the inherent relation of the components of the settlement space environment along China’s Grand Canal Tianjin Section and the inherent law influencing its current state. The research results show that the ecological factor of life along China’s Grand Canal exerted a positive influence on the ecological factor of production and the path coefficient was 0.344. The ecological factor of life and the factor of accessibility were in a relation of positive influence and the former had the most prominent influence on the latter, with a path coefficient of 0.534, while the path coefficient of the influence of the latter on the former was 0.131. The factor of social culture exerted a positive influence on the ecological factor of life, with the path coefficient being 0.765. The research conclusion analyzed the realistic difficulties of the settlement space environment along China’s Grand Canal, revealed the inherent law between different surveyed factors and provided basic reference for feature extraction, evaluation and optimized development of the settlement space along China’s Grand Canal.Spatial Planning and Strateg

Optimization of culturing conditions for isolated Arthrobacter sp. ZXY-2, an effective atrazine-degrading and salt-adaptive bacterium

The increasing salinity in aquatic environments has had a negative impact on the biodegradation of atrazine, an extensively used herbicide which has been proven to pollute soil and water ecosystems. In the present study, a novel atrazine-degrading strain (ZXY-2) was isolated from industrial wastewater and identified as the Arthrobacter genus with the 16S rRNA gene. Results indicated that the strain showed a high salinity tolerance, and was able to tolerate NaCl concentrations up to 10% (w/w). Plackett–Burman (PB) multifactorial design and response surface methodology (RSM) were then employed to optimize the culturing conditions. Results showed that among the selected fifteen factors, six contributing factors were obtained. Subsequently, by employing the RSM to model and optimize atrazine degradation, a biodegradation efficiency of 12.73 mg L−1 h−1 was reached under optimal conditions (34.04 °C, pH 9.0, inoculum size 10% (v/v), 2.212 g L−1 of sucrose, 6 g L−1 of Na2HPO4·12H2O, and 50 mg L−1 of atrazine). In addition, a statistically quadratic polynomial mathematical model was suggested (R2 = 0.9873). In contrast to other atrazine-degrading bacteria, ZXY-2 appears to be adapted to life under high salinity conditions and sustains excellent atrazine degradation performance. Therefore it could potentially be applied in atrazine bioremediation.Sanitary Engineerin

In-Site Phenotype of the Settlement Space along China’s Grand Canal Tianjin Section: GIS-sDNA-Based Model Analysis

The settlement space along China’s Grand Canal composes an important part of the Canal heritage, has a close bearing on the production and life of the residents there, nourishes rich culture and wisdom and boasts vital value of conservation and inheritance. Due to China’s rapid urbanization and industrialization, the settlements along the canal have been destroyed to some extent and their in-site characteristics urgently need excavation and conservation. Through field investigation, space syntax and GIS analysis, this paper performs quantitative analysis of the in-site characteristics of 18 typical rural settlements there. The findings show that: (1) The settlement space of industry dominant type for commerce and trade is comparatively dynamic and the capacity of topology and integration and the attractive force of the settlement center are stronger. (2) The dynamic scope of the citizens’ everyday traveling in the settlements has the closest correlation with the data of public-service facilities. (3) The settlements along the canal boast multiple, causal and blended in-site phenotype. The research findings provide new standards to categorize the settlements along China’s Grand Canal, paths and methods to explore the characteristics of the settlements and new cognitive perspectives to conserve and renew the settlements along China’s Grand Canal Tianjin Section.Spatial Planning and Strateg

A Dynamically Reconfigurable Recursive Switched-Capacitor DC-DC Converter with Adaptive Load Ability Enhancement

Multiple voltage conversion ratio (VCR) recursive switched-capacitor (SC) dc-dc converters, based on several basic 2:1 converters, are widely used for on-chip power supplies due to their flexible VCRs for higher energy efficiency. However, conventional multiple VCR SC converters usually have one or more 2:1 converters unused for some VCRs, which results in lower power density and chip area wastage. This article presents a new recursive dc-dc converter system, which can dynamically reconfigure the connection of all on-chip 2:1 converter cells so that the unused converters in the conventional designs can be reused in this new architecture for increasing the load-driving capacity, power density, and power efficiency. To validate the design, a 4-bit-input 15-ratio system was designed and fabricated in a 180-nm BCD process, which can support a maximum load current of \text{0.71}\,\text{mA} and achieves a peak power efficiency of 93.1% with 105.3\,\mu \text{A/mm} {2} chip power density from a 2-V input power supply. The measurement results show that the load-driving capacity can become 6.826×, 2.236×, and 2.175× larger than the conventional topology when the VCR is 1/2, 1/4, and 3/4, respectively. In addition, the power efficiency under these specific VCRs can also be improved considerably.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic Instrumentatio

Design and assessments on a hybrid pin fin-metal foam structure towards enhancing melting heat transfer: An experimental study

Solar energy, as a kind of renewable energy, offers a large reserve to be harvested at a reasonably low cost for engineering applications. To decouple the temporal and spatial relevance of the continuous energy supply of solar energy, latent heat thermal energy storage can deal with this problem at different temperatures. Aiming to improve energy efficiency, a novel hybrid metal foam-pin fin structure is designed and assessed. Upon conducting measurements on a well-designed experimental bench, the phase change processes of paraffin that is filled in fins, metal foam, and a combination of both (hybrid structure) are evaluated. During the experiments, the transient melting interface is snapshotted and temperature development is documented under five different heat source temperatures of 61 °C, 63 °C, 65 °C, 68 °C, and 70 °C. In the foreground of the novel hybrid structure, each segment of the hybrid is also justified and discussed. Results indicate that the hybrid structure augments marked heat transfer. Compared to pure PCM, complete melting time decreases by 63.4% and simultaneously the temperature response rate increases by 143.9% as implementing the hybrid. Attempts to design hybrid structure find a solution to assess and operate thermal storage applications for solar engineering.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Process and Energ

A Single-Stage Three-Mode Reconfigurable Regulating Rectifier for Wireless Power Transfer

In this article, we propose a reconfigurable regulating rectifier with a wide operational range for wireless power transfer. The proposed three-mode rectifier achieves a broad range voltage regulation without global loop control to minimize the chip area occupation. Compared with previous work, more working modes and greater voltage gain allow the proposed rectifier to regulate lower input power, which extends the voltage regulation range. A local loop control scheme is proposed for voltage rectification with three modes. It adaptively senses the duty cycle of the mode signal to determine the working mode of the rectifier, and configure the rectifier to the desired mode for voltage regulation. The proposed system was designed and fabricated in a 180-nm BCD technology with an active area of 1.17 mm2. The measurement results show that the proposed system can rectify wide-range input ac power to a regulated output. The achieved voltage conversion ratiois between 0.95X and 2.68X, with a peak power conversion efficiencyat 87.4%.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic Instrumentatio

Reviving the rock-salt phases in Ni-rich layered cathodes by mechano-electrochemistry in all-solid-state batteries

The rock-salt phase (RSP) formed on the surface of Ni-rich layered cathodes in liquid-electrolyte lithium-ion batteries is conceived to be electrochemically "dead". Here we show massive RSP forms in the interior of LiNixMnyCo(1−x-y)O2 (NMC) crystals in sulfide based all solid state batteries (ASSBs), but the RSP remains electrochemically active even after long cycles. The RSP and the layered structure constitute a two-phase mixture, a material architecture that is distinctly different from the RSP in liquid electrolytes. The tensioned layered phase affords an effective percolation channel into which lithium is squeezed out of the RSPs by compressive stress, rendering the RSPs electrochemically active. Consequently, the ASSBs with predominant RSP in the NMC cathode deliver remarkable long cycle life of 4000 cycles at high areal capacity of 4.3 mAh/cm2. Our study unveils distinct mechano-electrochemistry of RSPs in ASSBs that can be harnessed to enable high energy density and durable ASSBs.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.RST/Storage of Electrochemical Energ