Suppression of nitric oxide (NO)-dependent behavior by double-stranded RNA-mediated silencing of a neuronal NO synthase gene

We have used double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to disrupt neuronal nitric oxide (NO) synthase (nNOS) gene function in the snail Lymnaea stagnalis and have detected a specific behavioral phenotype. The injection of whole animals with synthetic dsRNA molecules targeted to the nNOS-encoding mRNA reduces feeding behavior in vivo and fictive feeding in vitro and interferes with NO synthesis by the CNS. By showing that synthetic dsRNA targeted to the nNOS mRNA causes a significant and long-lasting reduction in the levels of Lym-nNOS mRNA, we verify that specific RNAi has occurred. Importantly, our results establish that the expression of nNOS gene is essential for normal feeding behavior. They also show that dsRNA can be used in the investigation of functional gene expression in the context of whole animal behavior, regardless of the availability of targeted mutation technologies

Interneuronal mechanism for Tinbergen's hierarchical model of behavioral choice

Recent studies of behavioral choice support the notion that the decision to carry out one behavior rather than another depends on the reconfiguration of shared interneuronal networks. We investigated another decision-making strategy, derived from the classical ethological literature, which proposes that behavioral choice depends on competition between autonomous networks. According to this model, behavioral choice depends on inhibitory interactions between incompatible hierarchically organized behaviors. We provide evidence for this by investigating the interneuronal mechanisms mediating behavioral choice between two autonomous circuits that underlie whole-body withdrawal and feeding in the pond snail Lymnaea. Whole-body withdrawal is a defensive reflex that is initiated by tactile contact with predators. As predicted by the hierarchical model, tactile stimuli that evoke whole-body withdrawal responses also inhibit ongoing feeding in the presence of feeding stimuli. By recording neurons from the feeding and withdrawal networks, we found no direct synaptic connections between the interneuronal and motoneuronal elements that generate the two behaviors. Instead, we discovered that behavioral choice depends on the interaction between two unique types of interneurons with asymmetrical synaptic connectivity that allows withdrawal to override feeding. One type of interneuron, the Pleuro-Buccal (PlB), is an extrinsic modulatory neuron of the feeding network that completely inhibits feeding when excited by touch-induced monosynaptic input from the second type of interneuron, Pedal-Dorsal12 (PeD12). PeD12 plays a critical role in behavioral choice by providing a synaptic pathway joining the two behavioral networks that underlies the competitive dominance of whole-body withdrawal over feeding

“I expect it as part of the kind of package deal when you sign up to these things” - Motivations and Experiences of Ghosting

Most online dating users perceive ghosting to be common and expect that there is a chance of being ghosted on online dating platforms (ODPs). The current study extends previous research by gaining qualitative insight into what people believe constitutes ghosting behaviour, why people ghost, and how ghosting makes them feel. This study aimed to 1) explore individuals' motivations to ghost, 2) explore individuals experiences of ghosting and 3) gather the ghosters views of ghosting definition. A total of 12 online interviews were conducted. All participants had previously ghosted on ODPs and lived in the UK. Data was analysed using reflective thematic analysis. The presented five themes reflect a contextual realist approach, using both semantic and latent coding, and reveal that ghosting is considered the norm on ODP. There are general and specific motivations underpinning ghosting behaviour, producing a mixed emotional response from the ghoster. The findings also shed light on how we can better define ghosting, with participants having concerns with the word relationship. Finally, we highlight several protective factors that can minimise the likelihood of ghosting. Based on our findings we suggest that ghosting be defined as being a gradual or sudden one sided ceasing of communication to end the progress of an interaction with another person. While we found several protective factors that can minimise the likelihood of ghosting, these are unique to the individual and ghosting cannot be abolished as it has become a normative and embedded practice within ODP

Beelines: Joyce’s apian aesthetics

This article examines the presence of apian life in James Joyce’s body of work in light of Maurice Maeterlinck’s discovery at the turn of the twentieth-century that honeybees communicate using a complex system of language. In December 1903, Joyce offered to translate Maeterlinck’s book-length study La Vie Des Abeille (The Life of the Bee) (1901) for the Irish Bee-Keeper, and the pages of the journal later resurface on a book-cart in Ulysses. Beginning with a discussion of the ‘economy of bee life’ in Stephen Hero, this article explores Joyce’s career-long fascination with nonhuman modes of communication, tracing his fascination with apian intelligence through close readings of Bloom’s bee-sting in Ulysses, as well as through the swarm of references that appear in Finnegans Wake. Finally, it argues that bees offer new ways of reading Joyce’s work, opening up new lines of connection between the fields of literary criticism and apiculture, and drawing the reader’s attention to the peripheral hum or murmur at the edges of human speech

Cardiometabolic health in people with HIV: expert consensus review

Objectives To develop consensus data statements and clinical recommendations to provide guidance for improving cardiometabolic health outcomes in people with HIV based on the knowledge and experience of an international panel of experts.Methods A targeted literature review including 281 conference presentations, peer-reviewed articles, and background references on cardiometabolic health in adults with HIV published between January 2016 and April 2022 was conducted and used to develop draft consensus data statements. Using a modified Delphi method, an international panel of 16 experts convened in workshops and completed surveys to refine consensus data statements and generate clinical recommendations.Results Overall, 10 data statements, five data gaps and 14 clinical recommendations achieved consensus. In the data statements, the panel describes increased risk of cardiometabolic health concerns in people with HIV compared with the general population, known risk factors, and the potential impact of antiretroviral therapy. The panel also identified data gaps to inform future research in people with HIV. Finally, in the clinical recommendations, the panel emphasizes the need for a holistic approach to comprehensive care that includes regular assessment of cardiometabolic health, access to cardiometabolic health services, counselling on potential changes in weight after initiating or switching antiretroviral therapy and encouraging a healthy lifestyle to lower cardiometabolic health risk.Conclusions On the basis of available data and expert consensus, an international panel developed clinical recommendations to address the increased risk of cardiometabolic disorders in people with HIV to ensure appropriate cardiometabolic health management for this population

A heuristic approach for inter-facility comparison of results from round robin testing of a floating wind turbine in irregular waves

This paper introduces metrics developed for analysing irregular wave test results from the round robin testing campaign carried out on a floating wind turbine as part of the EU H2020 MaRINET2 project. A 1/60th scale model of a 10 MW floating platform was tested in wave basins in four different locations around Europe. The tests carried out in each facility included decay tests, tests in regular and irregular waves with and without wind thrust, and tests to characterise the mooring system as well as the model itself. While response amplitude operations (RAOs) are a useful tool for assessing device performance in irregular waves, they are not easy to interpret when performing an inter-facility comparison where there are many variables. Metrics that use a single value per test condition rather than an RAO curve are a means of efficiently comparing tests from different basins in a more heuristic manner. In this research, the focus is on using metrics to assess how the platform responds with varying wave height and thrust across different facilities. It is found that the metrics implemented are very useful for extracting global trends across different basins and test conditions

The twilight of the Liberal Social Contract? On the Reception of Rawlsian Political Liberalism

This chapter discusses the Rawlsian project of public reason, or public justification-based 'political' liberalism, and its reception. After a brief philosophical rather than philological reconstruction of the project, the chapter revolves around a distinction between idealist and realist responses to it. Focusing on political liberalism’s critical reception illuminates an overarching question: was Rawls’s revival of a contractualist approach to liberal legitimacy a fruitful move for liberalism and/or the social contract tradition? The last section contains a largely negative answer to that question. Nonetheless the chapter's conclusion shows that the research programme of political liberalism provided and continues to provide illuminating insights into the limitations of liberal contractualism, especially under conditions of persistent and radical diversity. The programme is, however, less receptive to challenges to do with the relative decline of the power of modern states

Constitutivism

A brief explanation and overview of constitutivism

Measurements of fiducial and differential cross sections for Higgs boson production in the diphoton decay channel at s√=8 TeV with ATLAS

Measurements of fiducial and differential cross sections are presented for Higgs boson production in proton-proton collisions at a centre-of-mass energy of s√=8 TeV. The analysis is performed in the H → γγ decay channel using 20.3 fb−1 of data recorded by the ATLAS experiment at the CERN Large Hadron Collider. The signal is extracted using a fit to the diphoton invariant mass spectrum assuming that the width of the resonance is much smaller than the experimental resolution. The signal yields are corrected for the effects of detector inefficiency and resolution. The pp → H → γγ fiducial cross section is measured to be 43.2 ±9.4(stat.) − 2.9 + 3.2 (syst.) ±1.2(lumi)fb for a Higgs boson of mass 125.4GeV decaying to two isolated photons that have transverse momentum greater than 35% and 25% of the diphoton invariant mass and each with absolute pseudorapidity less than 2.37. Four additional fiducial cross sections and two cross-section limits are presented in phase space regions that test the theoretical modelling of different Higgs boson production mechanisms, or are sensitive to physics beyond the Standard Model. Differential cross sections are also presented, as a function of variables related to the diphoton kinematics and the jet activity produced in the Higgs boson events. The observed spectra are statistically limited but broadly in line with the theoretical expectations

Search for squarks and gluinos in events with isolated leptons, jets and missing transverse momentum at s√=8 TeV with the ATLAS detector

The results of a search for supersymmetry in final states containing at least one isolated lepton (electron or muon), jets and large missing transverse momentum with the ATLAS detector at the Large Hadron Collider are reported. The search is based on proton-proton collision data at a centre-of-mass energy s√=8 TeV collected in 2012, corresponding to an integrated luminosity of 20 fb−1. No significant excess above the Standard Model expectation is observed. Limits are set on supersymmetric particle masses for various supersymmetric models. Depending on the model, the search excludes gluino masses up to 1.32 TeV and squark masses up to 840 GeV. Limits are also set on the parameters of a minimal universal extra dimension model, excluding a compactification radius of 1/R c = 950 GeV for a cut-off scale times radius (ΛR c) of approximately 30