Single cell transcriptome analysis using next generation sequencing.

The heterogeneity of tissues, especially in cancer research, is a central issue in transcriptome analysis. In recent years, research has primarily focused on the development of methods for single cell analysis. Single cell analysis aims at gaining (novel) insights into biological processes of healthy and diseased cells. Some of the challenges in transcriptome analysis concern low abundance of sample starting material, necessary sample amplification steps and subsequent analysis. In this study, two fundamentally different approaches to amplification were compared using next-generation sequencing analysis: I. exponential amplification using polymerase-chain-reaction (PCR) and II. linear amplification. For both approaches, protocols for single cell extraction, cell lysis, cDNA synthesis, cDNA amplification and preparation of next-generation sequencing libraries were developed. We could successfully show that transcriptome analysis of low numbers of cells is feasible with both exponential and linear amplification. Using exponential amplification, the highest amplification rates up to 106 were possible. The reproducibility of results is a strength of the linear amplification method. The analysis of next generation sequencing data in single cell samples showed detectable expression in at least 16.000 genes. The variance between samples results in a need to work with a greater amount of biological replicates. In summary it can be said that single cell transcriptome analysis with next generation sequencing is possible but improvements leading to a higher yield of transcriptome reads is required. In the near future by comparing single cancer cells with healthy ones for example, a basis for improved prognosis and diagnosis can be realised

Improving the understandability of the next edition of the International System of Units (SI) by focusing on its conceptual structure

The International System of Units (SI) is fundamental for the social, and not only the scientific, role of metrology, and as such its understandability is a crucial issue. According to the current draft of the new SI Brochure, the next edition of the SI will be significantly more complex in its conceptual structure than the previous ones. Identifying a strategy for effectively communicating its main contents is then a worthwhile endeavor, in order to increase the acceptance and thus the sustainability of the SI itself. Our proposal is to focus on the semantic structure of the definitions: this is instrumental to the awareness campaigns recommended by the General Conference on Weights and Measures to make the next edition of the SI understandable by a diverse readership without compromising scientific rigor.Comment: 10 pages, 1 table, accepted on Journal MEASUREMEN

Quantum Double-Torus

A symmetry extending the $T^2$-symmetry of the noncommutative torus $T^2_q$ is studied in the category of quantum groups. This extended symmetry is given by the quantum double-torus defined as a compact matrix quantum group consisting of the disjoint union of $T^2$ and $T^2_{q^2}$. The bicross-product structure of the polynomial Hopf algebra of the quantum double-torus is computed. The Haar measure and the complete list of unitary irreducible representations of the quantum double-torus are determined explicitly.Comment: 6 pages, no figures, amslatex, reformatted for Comptes Rendus, references added, typos and French correcte

Heat Conduction Process on Community Networks as a Recommendation Model

Using heat conduction mechanism on a social network we develop a systematic method to predict missing values as recommendations. This method can treat very large matrices that are typical of internet communities. In particular, with an innovative, exact formulation that accommodates arbitrary boundary condition, our method is easy to use in real applications. The performance is assessed by comparing with traditional recommendation methods using real data.Comment: 4 pages, 2 figure

Supernatural Cosmic Origins: Challenging the Reigning Paradigm

Contemporary scientific study primarily uses a paradigm based upon naturalism, materialism, and empiricism on which to base research. The widely accepted cosmological model the big bang theory adheres to this paradigm. Despite many weaknesses in this model and in the paradigm itself, researchers continue to favor the modification of the accepted model over the adoption of other more comprehensive models. The paradigm from which the models proposed by Russell Humphries, John Hartnett, and Jason Lisle come justifies the six-day creation young-earth biblical account and better fits observational evidence with fewer arbitrary assumptions than the paradigm from which the big bang theory comes