6 research outputs found
Rapid Evolution of Silver Nanoparticle Resistance in Escherichia Coli
- Author
- Publication venue
- Aggie Digital Collections and Scholarship
- Publication date
- 01/01/2015
- Field of study
The recent exponential increase in the use of engineered nanoparticles (eNPs) means both greater intentional and unintentional exposure of eNPs to microbes. Intentional use includes the use of eNPs as biocides. Unintentional exposure results from the fact that eNPs are included in a variety of commercial products (paints, sunscreens, cosmetics). Many of these eNPs are composed of heavy metals or metal oxides such as silver, gold, zinc, titanium dioxide, and zinc oxide. It is thought that since metallic/metallic oxide NPs impact so many aspects of bacterial physiology that it will difficult for bacteria to evolve resistance to them. This study utilized laboratory experimental evolution to evolve silver nanoparticle (AgNP) resistance in the bacterium Escherichia coli (K-12 MG1655), a bacterium that does not harbor any known silver resistance elements. After 225 generations of exposure to the AgNP environment, the treatment populations demonstrated greater fitness vs. control strains as measured by optical density (OD) and colony forming units (CFU) in the presence of varying concentrations of 10 nm citrate-coated silver nanoparticles (AgNP) or silver nitrate (AgNO3). Genomic analysis shows that changes associated with AgNP resistance were already accumulating within the treatment populations by generation 100, and by generation 200 three mutations had swept to high frequency in the AgNP resistance stocks. This study indicates that despite previous claims to the contrary bacteria can easily evolve resistance to AgNPs, and this occurs by relatively simple genomic changes. These results indicate that care should be taken with regards to the use of eNPs as biocides as well as with regards to unintentional exposure of microbial communities to eNPs in waste products
An integrated command and control architecture concept for unmanned systems in the year 2030
- Author
- Ang Kha Luna
- Ang Teo Hong
- Buckley Omari D.
- Chia Boon Chye
- Chia Tommy
- Costica Yionon
- Cunningham Dustin
- Fontenot Dion G.
- Gho Delvin
- Ho Liang Yoong
- Johnson Jamarr J.
- Lim Han Wei
- Lim Wei Han
- Lo Chee Hun
- Lu Chin Leong
- Matthews Adam
- Moran Michael G.
- Ng Wei Gee
- Ng Yeow Cheng
- Quah Raymond
- Quek Chee Luna
- Quincy Keith E.
- SEA Cohort SEA-16
- Seet Henry
- Tan Chin Wah John
- Tan Wei Chieh
- Tham Gabriel
- Thompson Bradley G.
- Ting Chi Yon
- Toh Boo Pin
- Tong Kee Leong
- Wee Yean Wee
- Wong Jason
- Wong Ka-Yoon
- Publication venue
- Monterey, California. Naval Postgraduate School
- Publication date
- 01/06/2010
- Field of study
U.S. Forces require an integrated Command and Control Architecture that enables operations of a dynamic mix of manned and unmanned systems. The level of autonomous behavior correlates to: 1) the amount of trust with the reporting vehicles, and 2) the multi-spectral perspective of the observations. The intent to illuminate the architectural issues for force protection in 2030 was based on a multi-phased analytical model of High Value Unit (HVU) defense. The results showed that autonomous unmanned aerial vehicles are required to defeat high-speed incoming missiles. To evaluate the level of autonomous behavior required for an integrated combat architecture, geometric distributions were modeled to determine force positioning, based on a scenario driven Detect-to-Engage timeline. Discrete event simulation was used to schedule operations, and a datalink budget assessment of communications to determine the critical failure paths in the the integrated combat architecture. The command and control principles used in the integrated combat architecture were based on Boyd's OODA (Obseve, Orient, Decide, and Act) Loop. A conservative fleet size estimate, given the uncertainties of the coverage overlap and radar detection range, a fleet size of 35 should be anticipated given an UAV detection range of 20km and radar coverage overlap of 4 seconds.http://archive.org/details/anintegratedcomm109455244US Navy (USN) authorsApproved for public release; distribution is unlimited
Photocatalytic removal of atrazine using N-doped TiO2 supported on phosphors
- Author
- Acharya
- Alemany
- Andersen
- Anon
- Antoniadou
- Chan
- Changseok Han
- Chen
- Ciambelli
- Cunningham
- Di Valentin
- Diana Sannino
- Dionysios D. Dionysiou
- Franco
- Gao
- Gianturco
- Guillén-Santiago
- Guo
- Han
- Harris
- Huang
- Karvaly
- Khan
- Kim
- Kim
- Konstantinou
- Labiadh
- Lackhoff
- Livraghi
- Ma
- McMurray
- Murakami
- Murcia
- Nilsen
- Olga Sacco
- Ozaki
- Palma
- Parra
- Pelizzetti
- Pelizzetti
- Petrier
- Qin
- Quincy
- Rizzo
- Rockafellow
- Sacco
- Sannino
- Sannino
- Sannino
- Sato
- Texier
- Vaiano
- Vaiano
- Vaiano
- Vincenzo Vaiano
- Yu
- Publication venue
- 'Elsevier BV'
- Publication date
- Field of study
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- Publication venue
- 'Duke University Press'
- Publication date
- 01/01/1997
- Field of study
The formation of college English: A survey of the archives of eighteenthâcentury rhetorical theory and practice
- Author
- _____
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- Aarsleff Hans
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- Publication venue
- 'Informa UK Limited'
- Publication date
- Field of study
1996 Annual Selected Bibliography
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- 1996 National Asian Pacific American Political Almanac.
- Abraham Margaret
- Accinelli Laura
- Adair L. S.
- Adamo Teresa
- Adier Shelley
- Adleman Jeanne
- Adler Shelley
- Ago Arthur Emmanuel
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- Vo Linda Trinh
- Vol
- Vuong Theresa Ahn
- Walker-Moffat Wendy
- Wallace Charles P
- Wallace Mara
- Wang
- Wang N. Y.
- Wang Nancy
- Wardle J.
- Watanabe M. E.
- Watanabe Sylvia
- Weaver Donna R
- Wei J.
- Weigle D. S.
- Weingberg Neil
- Westermeyer Joseph
- Westermeyer Joseph
- Wewers M. E.
- Whalen Jeanne
- White George
- Whittemore A. S.
- Wiecha J. M.
- Wiener Jon
- Wilkinson Alec
- Williams Walter L
- Wilson
- Wilson Robin
- Wirtz Rosette Ho
- WithNezu Eyes
- Wong Bill
- Wong Gerrye
- Wong Lloyd L
- Wong Paul
- Wong Roger R
- Wong Sau-Ling C
- Wong Shawn
- Woo Elaine
- Wooden Wayne S
- Wu A. H. A. S.
- Wu Frank H
- Wu Frank H
- Wu-Tso P.
- Wynter Leon E
- Wynter Leon E
- Xiao David
- Yagi Darryl Takizo
- Yamanaka Lois-Ann
- Yeh Theresa
- Yeo G.
- Yi Jenny
- Yip Alethea
- Yip Alethea
- Yip Alethea
- Yip Alethea
- Yip Alethea
- Yip Alethea
- Yoon Cynthia
- Yoon Eunice
- Yoon In-Tin
- Yoon Rita
- Yoshimura Kari
- Young Jeffry J
- Yount David E
- Yu X. J.
- Yu Yan
- Yung Judy
- Yzaguirre Raul
- Zia Helen
- Ziegler R.
- Zimmerman Sarah Midori
- Zuker R. Fred.
- Publication venue
- 'UCLA American Indian Studies Center'
- Publication date
- Field of study