Collaborative applications used in a wireless environment at sea for use in Coast Guard Law Enforcement and Homeland Security missions

This thesis analyzes the potential impact of incorporating wireless technologies, specifically an 802.11 mesh layer architecture and 802.16 Orthogonal Frequency Division Multiplexing, in order to effectively and more efficiently transmit data and create a symbiotic operational picture between Coast Guard Cutters, their boarding teams, Coast Guard Operation Centers, and various external agencies. Two distinct collaborative software programs, Groove Virtual Office and the Naval Postgraduate School's Situational Awareness Agent, are utilized over the Tactical Mesh and OFDM network configurations to improve the Common Operating Picture of involved units within a marine environment to evaluate their potential impact for the Coast Guard. This is being done to increase the effectiveness and efficiency of Coast Guard units while they carry out their Law Enforcement and Homeland Security Missions. Through multiple field experiments, including Tactical Network Topology and nuclear component sensing with Lawrence Livermore National Laboratory, we utilize commercial off the shelf (COTS) equipment and software to evaluate their impact on these missions.http://archive.org/details/collaborativeppl109452311Lieutenant Commander, United States Coast GuardLieutenant, United States Coast GuardApproved for public release; distribution is unlimited

The Oxidative Stress Network of Mycobacterium tuberculosis Reveals Coordination between Radical Detoxification Systems

SummaryM. tuberculosis (Mtb) survives a hostile environment within the host that is shaped in part by oxidative stress. The mechanisms used by Mtb to resist these stresses remain ill-defined because the complex combination of oxidants generated by host immunity is difficult to accurately recapitulate in vitro. We performed a genome-wide genetic interaction screen to comprehensively delineate oxidative stress resistance pathways necessary for Mtb to resist oxidation during infection. Our analysis predicted functional relationships between the superoxide-detoxifying enzyme (SodA), an integral membrane protein (DoxX), and a predicted thiol-oxidoreductase (SseA). Consistent with that, SodA, DoxX, and SseA form a membrane-associated oxidoreductase complex (MRC) that physically links radical detoxification with cytosolic thiol homeostasis. Loss of any MRC component correlated with defective recycling of mycothiol and accumulation of cellular oxidative damage. This previously uncharacterized coordination between oxygen radical detoxification and thiol homeostasis is required to overcome the oxidative environment Mtb encounters in the host