Role of N-glycosylation in oral cancer

Oral squamous cell carcinoma represents more than 90% head and neck cancers with high incidence rate and morbidity. To date, little is known about the molecular mechanisms responsible for OSCC initiation and progression to advanced disease. Thus, identifying key pathways involved in OSCC pathobiology is likely to lead to the identification of new druggable targets and future anti-cancer therapies. Work from our laboratory has linked the metabolic pathway of protein N-glycosylation with OSCC biology. Specifically, overexpression of the first N-glycosylation gene, DPAGT1, in human OSCC tumor specimens was shown to be associated with aberrant activation of canonical Wnt signaling and inhibition of mature E-cadherin junctions. The purpose of this study was to examine how increased N-glycosylation was associated with OSCC growth and metastatic properties in cellular and murine models. We show that high level of DPAGT1 expression correlates with increased cell surface modification of malignant OSCC HSC-3 cells with complex N-glycans. Further, HSC-3 cells are hypersenitive to the N-glycosylation inhibitor, tunicamycin, suggesting that aggressive properties of OSCC cells depend, in part, on the N-glycosylation pathway. Lastly, we show that orthotopic HSC-3 cell-derived tumor xenografts are inhibited by tunicamycin both in overall growth and metastases, indicating that targeting DPAGT1 and N-glycosylation may represent a new strategy for the treatment of OSCC in human patients

INDEPENDENT ORIGINATION OF FLORAL ZYGOMORPHY, A PREDICTED ADAPTIVE RESPONSE TO POLLINATORS: DEVELOPMENTAL AND GENETIC MECHANISMS

Observations of floral development indicate that floral organ initiation in pentapetalous flowers more commonly results in a medially positioned abaxial petal (MAB) than in a medially positioned adaxial petal (MAD), where the medial plane is defined by the stem and the bract during early floral development. It was proposed that the dominant MAB petal initiation might impose a developmental constraint that leads to the evolution of limited patterns of floral zygomorphy in Asteridae, a family in which the floral zygomorphy develops along the medial plane and results in a central ventral (CV) petal in mature flowers. Here, I investigate whether the pattern of floral organ initiation may limit patterns of floral zygomorphy to evolve in pentapetalous angiosperms. I analyzed floral diagrams representing 405 species in 330 genera of pentapetalous angiosperms to reconstruct the evolution of floral organ initiation and the evolution of developmental processes that give rise to floral zygomorphy on a phylogenetic framework. Results indicate that MAB petal initiation is the most common; it occupies 86.2% of diversity and represents the ancestral state of floral organ initiation in pentapetalous angiosperms. The MAD petal initiation evolved 28 times independently from the ancestral MAB petal initiation. Among the 34 independent originations of floral zygomorphy, 76.5% of these clades represent MAB petal initiation, among which only 47% of the clades result a CV petal in mature flowers. The discrepancy is explained by the existence of developmental processes that result in floral zygomorphy along oblique planes of floral symmetry in addition to along the medial plane. Findings suggest that although the early floral organ initiation plays a constraining role to the evolution of patterns of floral zygomorphy, the constraint diverges along phylogenetically distantly related groups that allow the independent originations of floral zygomorphy through distinct development processes in pentapetalous angiosperms. In additional study, the butterfly-like flowers of Schizanthus are adapted to pollination by bees, hummingbirds, and moths. I investigated the genetic basis of the zygomorphic corolla, for which development is key to the explosive pollen release mechanism found in the species of Schizanthus adapted to bee pollinators. I examined differential gene expression profiles across the zygomorphic corolla of Schizanthus pinnatus, a bee-pollinated species, by analyzing RNA transcriptome sequencing (RNA- seq). Data indicated that CYC2 is not expressed in the zygomorphic corolla of Sc. pinnatus, suggesting CYC2 is not involved in the development of floral zygomorphy in Schizanthus (Solanaceae). The data also indicated that a number of genes are differentially expressed across the corolla

Peningkatan prestasi hasil belajar siswa pada mata pelajaran pendidikan agama islam materi nama-nama hari akhir dengan strategi make a match di kelas VI SDN 001 Tanah Grogot 2012/2013

Penelitian ini dilatarbelakangi oleh rendahnya prestasi hasil belajar siswa terhadap mata pelajaran pendidikan agama islam. Hal ini berdasarkan hasil nilai tes sebelum perbaikan, hasil nilai rata-rata sebelum perbaikan rendah yaitu 58,8 (ketuntasan belajar hanya mencapai 7,7%).Tujuan penelitian ini adalah untuk mengetahui peningkatan prestasi hasil belajar siswa pada pelajaran pendidikan agama islam materi nama-nama hari akhir di kelas VI SDN 001 Tanah Grogot setelah diterapkan strategi make a match . Metode penelitian ini adalah penelitian tindakan kelas. Tindakan yang dilakukan terdiri dari tiga tindakan dalam tiga siklus. Tiap siklus terdiri dari empat tahapan yaitu perencanaan, pelaksanaan, pengamatan, dan refleksi. Adapun kelas yang diteliti adalah siswa kelas VI SDN 001 Tanah Grogot dengan jumlah siswa 26 orang. Nilai rata-rata sebelum perbaikan diperoleh nilai rata-rata 58,8 pada siklus 1 nilai rata-rata kelas meningkat menjadi 65,4. Pada siklus 2 nilai rata-rata 78,5 dan siklus 3 nilai rata-rata 90,4. Jadi dari siklus I ke siklus 3 terdapat peningkatan prestasi belajar. Ketuntasan belajar pada siklus I yaitu 23,1%, siklus II 65%, sedangkan pada siklus 3 yaitu 92%

KHALWAT DALAM PERSPEKTIF HUKUM ISLAM DAN HUKUM POSITIF

The existence of a man and woman who have no kinship so that it is lawful to marry her, in a lonely place without being ac companied by a mahram of the male or female side. This khalwat is a crime that is not subject to hudud punishment and kafarah punishment. This form of khalwat crime is included in the category of ta'zir finger whose number of punishment is not limited. In the Qur'an and Sunnah this khalwat act is highly reproached, but not clearly regulated in the Qur'an and Sunnah. So this act can be entered into the ta'zir group. All deeds that should (need) be forbidden to fulfill the common good (community). This prohibition must necessarily be made on the basis of community agreement / consensus in ways that are considered eligible. In North Aceh, the khalwat actors who are close to the power are hard to touch with the law, it is not surprising to all of us to remember that the law in this country is not yet the commander but the law is merely a bargaining position in everyday life

Implementation of Manuscript-based History Learning on High School Students Learning Outcomes in Langsa City

The study investigates the impact of history learning based on the Abdurrauf As-Singkili manuscript on student learning outcomes. The research addresses the limitations of history education in schools, particularly regarding the lack of engaging and locally relevant content. By utilizing the valuable historical resource of the Abdurrauf As-Singkili manuscript, the study aims to provide an innovative approach to history education that enhances student motivation and learning outcomes. The experimental research methodology involved two groups: an experimental group that received history instruction using the manuscript and a control group that did not. The study was conducted in two high schools in Kota Langsa, Aceh, Indonesia, and the participants were X-grade students. A questionnaire assessed students' knowledge, and data analysis included hypothesis testing. The results indicate that the experimental group achieved higher average scores than the control group, suggesting the positive impact of utilizing the Abdurrauf As-Singkili manuscript in history education. The average score of students in SMA Negeri 1 Kota Langsa with the module was 85.54, while the average score of students in SMA Negeri 3 Kota Langsa without the module was 69.28. The statistical analysis showed that the distribution of post-test data for the experimental group can be considered normal. The findings contribute to developing innovative history teaching methods and highlight the significance of utilizing local historical resources in enriching students' learning experiences

Khalwat dalam Perspektif Hukum Islam dan Hukum Positif

The existence of a man and woman who have no kinship so that it is lawful to marry her, in a lonely place without being ac companied by a mahram of the male or female side. This khalwat is a crime that is not subject to hudud punishment and kafarah punishment. This form of khalwat crime is included in the category of ta'zir finger whose number of punishment is not limited. In the Qur'an and Sunnah this khalwat act is highly reproached, but not clearly regulated in the Qur'an and Sunnah. So this act can be entered into the ta'zir group. All deeds that should (need) be forbidden to fulfill the common good (community). This prohibition must necessarily be made on the basis of community agreement / consensus in ways that are considered eligible. In North Aceh, the khalwat actors who are close to the power are hard to touch with the law, it is not surprising to all of us to remember that the law in this country is not yet the commander but the law is merely a bargaining position in everyday life

IMPLEMENTASI HUKUM ISLAM DI ACEH DALAM PERSPEKTIF HISTORIS

Penegakan hukum Islam di Provinsi Acheh pada periode dahulu sampai sekarang dan yang akan datang akan mendapatkan dukungan dari masyarakat di Acheh. Dari pengalaman empiris masa lalu itu, dibuktikan dengan bahwa hukum Islam telah diterima dan dapat berfungsi sebagai hukum yang mengatur kehidupan masyarakat. Penegakan hukum Islam di Acheh setelah kemerdekaan pada awalnya diperjuangkan dengan melewati / melewati status Acheh sebagai Area pendukung diri sendiri. Provinsi oleh kewenangan adalah otonom untuk mengatur sendiri domestik dan menjalankan syariat Islam dengan sempurna dan pasti sekarang setelah melahirkan undang-undang kelahiran dan qanun-qanun syariat Islam maka penerapan syariat Islam di Acheh dapat dalam mewujudkan dengan sempurna. Penegakan hukum Islam di Provinsi Acheh memiliki dasar yang berlaku, karena secara historis-sosiologis adalah Syariat Islam di Acheh telah dieksekusi dalam kehidupan nyata sejak beberapa abad terakhir ketika Acheh masih dalam bentuk kekaisaran. Acheh Society, selama sejarah, telah menjadikan Islam sebagai pedoman dalam hidupnya. Melaksanakan dan perbuatan ajaran Islam dalam mencakup sejarah yang cukup lama yang telah melahirkan suasana masyarakat dan budaya Acheh yang Islami, oleh karena itu syariat Islam memiliki satu dan menjadi tuntunan hidup (living law) bagi masyarakat Ace

Understanding the factors that attract travellers to buy tickets online in Saudi Arabia

Despite widespread discussions of online consumer behaviour and the effect of web quality on online user’s actions, there is still a lack of research in the area of consumer attitude towards the services provided by airline companies due to the specific nature of travellers. – being using the internet for different motivations and buying specific kind of product (e-tickets). This study aims to measure consumers’ electronic satisfaction and intention to purchase tickets from Airlines websites. The results provide better understanding on the factors that attract travellers to adopt the most cost effective distribution channel for Airlines (own website) for ticketing needs. To obtain the study objective, a conceptual framework is developed based on literature pertaining to e-consumer behaviour, web quality, and travel and tourism streams. A detective quantitative methodology was chosen to examine the constructs and the relations within the framework. An online survey targeting actual airline online users (travellers) in the Kingdom of Saudi Arabia is suggested with items covering 9 constructs: Information Quality (IQ), System Quality (SQ), Perceived usefulness (PU), Perceived ease of use (PEOU),e-Trust (ET), Airline reputation (AR), Price Perception (PP), e-Satisfaction (ES), and Intention to Purchase (IP). Findings would help decision makers within airline companies to understand their customers’ online behaviour and enable enhancements and modifications to be made to their airline storefront, hence ensuring the satisfaction of potential customers and conversion of visitors into buyers