8 research outputs found
Permeability characteristics of the peritrophic membranes of Manduca sexta larvae
- Author
- Publication venue
- 'Elsevier BV'
- Publication date
- Field of study
Genetic studies of the pyrimidine permeases from Saccharomyces cerevisiae: Lack of intragenic complementation
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Inhibition of leucine transport in Saccharomyces by S-adenosylmethionine
- Author
- Publication venue
- 'American Society for Microbiology'
- Publication date
- Field of study
Homocysteine Methyltransferases Mht1 and Sam4 Prevent the Accumulation of Age-damaged (R,S)-AdoMet in the Yeast Saccharomyces cerevisiae*
- Author
- Publication venue
- American Society for Biochemistry and Molecular Biology
- Publication date
- Field of study
The biological methyl donor S-adenosyl-l-methionine (AdoMet) is spontaneously degraded by inversion of its sulfonium center to form the R,S diastereomer. Unlike its precursor, (S,S)-AdoMet, (R,S)-AdoMet has no known cellular function and may have some toxicity. Although the rate of (R,S)-AdoMet formation under physiological conditions is significant, it has not been detected at substantial levels in vivo in a wide range of organisms. These observations imply that there are mechanisms that either dispose of (R,S)-AdoMet or convert it back to (S,S)-AdoMet. Previously, we identified two homocysteine methyltransferases (Mht1 and Sam4) in yeast capable of recognizing and metabolizing (R,S)-AdoMet. We found similar activities in worms, plants, and flies. However, it was not established whether these activities could prevent R,S accumulation. In this work, we show that both the Mht1 and Sam4 enzymes are capable of preventing R,S accumulation in Saccharomyces cerevisiae grown to stationary phase; deletion of both genes results in significant (R,S)-AdoMet accumulation. To our knowledge, this is the first time that such an accumulation of (R,S)-AdoMet has been reported in any organism. We show that yeast cells can take up (R,S)-AdoMet from the medium using the same transporter (Sam3) used to import (S,S)-AdoMet. Our results suggest that yeast cells have evolved efficient mechanisms not only for dealing with the spontaneous intracellular generation of the (R,S)-AdoMet degradation product but for utilizing environmental sources as a nutrient
Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein
- Author
- Publication venue
- 'American Society for Microbiology'
- Publication date
- Field of study
A history of research on yeasts 13. The active transport of metabolites into yeasts
- Author
- Ahmad
- Akita
- Alcorn
- Aldermann
- Ali
- Alves-Araújo
- Amerik
- André
- Balzi
- Baranowski
- Bargues
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Barnett
- Baruffini
- Becker
- Beckerich
- Betina
- Billard
- Bisson
- Bisson
- Bisson
- Blasco
- Bode
- Boles
- Boles
- Boles
- Boller
- Bonekamp
- Brocklehurst
- Brocklehurst
- Burden
- Bussey
- Button
- Buziol
- Carlson
- Carter
- Carvalho-Silva
- Casal
- Casal
- Casal
- Caspari
- Celenza
- Chang
- Ciriacy
- Cockburn
- Cocking
- Coschigano
- Courchesne
- Crabeel
- Crespo
- Cássio
- Cássio
- Cássio
- Côrte-Real
- Côrte-Real
- Dabrowa
- Darte
- Davidova
- Davidova
- Davies
- Davson
- de Boer
- De Bruijne
- De Craene
- de Koning
- De la Fuente
- de Robichon Szulmajster
- DeJuan
- Deák
- Dickson
- Diderich
- Diezemann
- Dlugai
- Does
- Does
- Douglas
- Drillien
- Dubois
- Dürr
- Eddy
- Eddy
- Eddy
- Eddy
- Eddy
- Eddy
- Eddy
- Eddy
- Eddy
- Ehwald
- Fan
- Fantes
- Farcasanu
- Fonseca
- Foury
- Fuhrmann
- Gale
- Gale
- Gasnier
- Geber
- Gerós
- Gille
- Gits
- Gits
- Goffrini
- Goffrini
- Goffrini
- Gottschalk
- Gottschalk
- Gradova
- Grauslund
- Greasham
- Gregory
- Greig
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Grenson
- Gusel'nikova
- Hall
- Halvorson
- Halvorson
- Hammond
- Harold
- Harold
- Harris
- Harris
- Heiland
- Heiland
- Helliwell
- Hoffman
- Hofmann
- Horak
- Horák
- Horák
- Horák
- Hough
- Höfer
- Höfer
- Ikeda
- Indge
- Ingraham
- Isnard
- Janda
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- Jaspers
- Jaspers
- Jaspers
- Jauniaux
- Jauniaux
- Jayakumar
- Johnston
- Joiris
- Jørgensen
- Kakinuma
- Katzmann
- Keilin
- Khan
- Kihara
- Kilian
- Kilian
- Kilian
- Kjeldahl
- Kleinzeller
- Klöppel
- Klöppel
- Knudsen
- Ko
- Kotliar
- Kotliar
- Kotyk
- Kotyk
- Kotyk
- Kotyk
- Kotyk
- Kotyk
- Krogh
- Krogh
- Krogh
- Kruckeberg
- Kruckeberg
- Kruckeberg
- Kubitschek
- Lasko
- Lavi
- Leben
- Leberle
- Legrain
- Lewis
- Leão
- Liang
- Lichko
- Lichtenberg-Fraté
- Lodi
- Lodi
- Logan
- Loureiro-Dias
- Lucas
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- Lundegårdh
- Lundegårdh
- Lundegårdh
- Magasanik
- Magaña-Schwencke
- Magaña-Schwencke
- Mahlberg
- Mai
- Maizels
- Makuc
- Marini
- Marini
- Marshall-Carlson
- Marshall-Carlson
- Matile
- Maw
- Mehta
- Miersch
- Milkowski
- Mills
- Minehart
- Misra
- Mitchell
- Mitchell
- Mitchell
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- Mitchell
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- Morlion
- Morrison
- Mortimer
- Mosley
- Moye
- Murphy
- Mörtberg
- Mörtberg
- Nagel
- Neigeborn
- Neigeborn
- Nelissen
- Neuberger
- Niemietz
- Niemietz
- Nourani
- Okada
- Okada
- Okada
- Okorokov
- Oliveira
- Opekarová
- Osothsilp
- Pasteur
- Paulsen
- Peinado
- Peinado
- Petrotta-Simpson
- Pinkerton
- Polak
- Postma
- Postma
- Prevot
- Prior
- Rai
- Rainbow
- Ramos
- Ramos
- Ramos
- Regenberg
- Regenberg
- Reifenberger
- Reifenberger
- Reinders
- Rickenberg
- Riggs
- Riley
- Roberg
- Robertson
- Robins
- Roon
- Roon
- Roon
- Roon
- Rouillon
- Roush
- Roush
- Roush
- Royt
- Royt
- Royt
- Rytka
- Saiyid
- Sato
- Schmidt
- Schulz
- Schwartz
- Schwencke
- Schwencke
- Schwencke
- Seaston
- Seaston
- Selvi
- Serrano
- Serrano
- Shukla
- Sims
- Sims
- Singer
- Singh
- Skou
- Soares-Silva
- Soetens
- Sols
- Sols
- Sousa
- Sousa
- Spence
- Spencer-Martins
- Spencer-Martins
- Srb
- Srb
- Sreekrishna
- Stanbrough
- Stanbrough
- Stockdale
- Surdin
- Surdin
- Svihla
- Svihla
- Svihla
- Sychrová
- Sychrová
- Taghikhani
- Tanaka
- Tang
- Tansey
- Taylor
- Theodoris
- Tschopp
- Tullin
- Turoscy
- Turoscy
- van Belle
- van den Broek
- van den Broek
- van den Broek
- van den Broek
- van den Broek
- van den Broek
- van Urk
- Vandenbol
- Vandenbol
- Vandenbol
- Varma
- Verma
- Verma
- Vissers
- Vissers
- Wang
- Warth
- Wearn
- Weierstall
- Weirich
- Wendell
- Weusthuis
- Wiame
- Wiame
- Wieczorke
- Wiedemuth
- Wieland
- Wiemken
- Wiemken
- Wiemken
- Williamson
- Willstätter
- Wood
- Woost
- Wu
- Wésolowski-Louvel
- Yadan
- Ye
- Zhu
- Zmijewski
- Özcan
- Özcan
- Özcan
- Özcan
- Publication venue
- 'Wiley'
- Publication date
- 01/01/2008
- Field of study
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- Harden
- Harden
- Harden
- Harden
- Harden
- Harden
- Harden
- Harden
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- Hardie
- Harold
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- Heredia
- Herman
- Herman
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- Herzenberg
- Heslot
- Hestrin
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- Hill
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- Hoffmann
- Hoffmann
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- Hofmeister
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- Holliday
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- Holzer
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- Hopkins
- Hopkins
- Hoppe-Seyler
- Hopper
- Horak
- Horak
- Horak
- Horazdovsky
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- Horisberger
- Horowitz
- Horák
- Hu
- Hubbard
- Hughes
- Hughes
- Hull
- Hull
- Hull
- Huppert
- Huxley
- Huxley
- Hwang
- Hwang
- Hwang
- Hägele
- Hämmerle
- Höber
- Höfer
- Höfer
- Hübner
- Ibsen
- Ibsen
- Igarashi
- Ihde
- Ihde
- Ikeda
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- Pasteur
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- Pigman
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- Piontek
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- Spiegelman
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- Yushok
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- Zacharias
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