Reliability assessment of null allele detection: inconsistencies between and within different methods

Microsatellite loci are widely used in population genetic studies, but the presence of null alleles may lead to biased results. Here we assessed five methods that indirectly detect null alleles, and found large inconsistencies among them. Our analysis was based on 20 microsatellite loci genotyped in a natural population of Microtus oeconomus sampled during 8 years, together with 1200 simulated populations without null alleles, but experiencing bottlenecks of varying duration and intensity, and 120 simulated populations with known null alleles. In the natural population, 29% of positive results were consistent between the methods in pairwise comparisons, and in the simulated dataset this proportion was 14%. The positive results were also inconsistent between different years in the natural population. In the null-allele-free simulated dataset, the number of false positives increased with increased bottleneck intensity and duration. We also found a low concordance in null allele detection between the original simulated populations and their 20% random subsets. In the populations simulated to include null alleles, between 22% and 42% of true null alleles remained undetected, which highlighted that detection errors are not restricted to false positives. None of the evaluated methods clearly outperformed the others when both false positive and false negative rates were considered. Accepting only the positive results consistent between at least two methods should considerably reduce the false positive rate, but this approach may increase the false negative rate. Our study demonstrates the need for novel null allele detection methods that could be reliably applied to natural population

Mentoring Programmes as a successful instrument for career development of people with disabilities and disadvantaged

[Excerpt] For many years the Mentoring, as a form of support for young people without work experience, is well-known practice. In the past ten years the Mentoring has proved itself as a wide-applied method of support in the career of people with disabilities. A good example of successfully held Mentoring programme in Europe is the “Equal Employment Opportunities: Mentoring and Training for Disabled People and Employers” Project. It is a transnational initiative supported by the “Leonardo da Vinci” Programme of the European Commission. The project includes 3 European countries: Bulgaria, Greece and the UK. The duration of the project was 24 months

Shrinking a large dataset to identify variables associated with increased risk of Plasmodium falciparum infection in Western Kenya

Large datasets are often not amenable to analysis using traditional single-step approaches. Here, our general objective was to apply imputation techniques, principal component analysis (PCA), elastic net and generalized linear models to a large dataset in a systematic approach to extract the most meaningful predictors for a health outcome. We extracted predictors for Plasmodium falciparum infection, from a large covariate dataset while facing limited numbers of observations, using data from the People, Animals, and their Zoonoses (PAZ) project to demonstrate these techniques: data collected from 415 homesteads in western Kenya, contained over 1500 variables that describe the health, environment, and social factors of the humans, livestock, and the homesteads in which they reside. The wide, sparse dataset was simplified to 42 predictors of P. falciparum malaria infection and wealth rankings were produced for all homesteads. The 42 predictors make biological sense and are supported by previous studies. This systematic data-mining approach we used would make many large datasets more manageable and informative for decision-making processes and health policy prioritization

Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set

We report a measurement of the bottom-strange meson mixing phase \beta_s using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays in which the quark-flavor content of the bottom-strange meson is identified at production. This measurement uses the full data set of proton-antiproton collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity. We report confidence regions in the two-dimensional space of \beta_s and the B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2, -1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in agreement with the standard model expectation. Assuming the standard model value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +- 0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +- 0.009 (syst) ps, which are consistent and competitive with determinations by other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012

Linking factual and procedural knowledge in solving science problems: A case study in a thermodynamics course

Well-specified problems of the type presented boxed in the introduction to this article are extremely common in science courses. Unfortunately, this does not mean that students find them easy to solve, even when a teacher provides model answers to problems which differ only marginally (in the teacher's eyes) from those put before the students. The central difficulty with such courses is that they do not embody instructional principles that reflect students' need for “direction” in problem solving. In this article, we describe how the necessary heuristics and strategic knowledge were built into the remake of a conventional thermodynamics course. In contrast to mainstream American work on learning problem solving we chose to direct our curriculum reconstruction using the Gal'perin theory of stage-by-stage formation of mental actions and Landa's description of the “through” systematization of knowledge. As indicated by both, we first developed an integrated system of instructional objectives: a programme of actions and methods (PAM) to solve problems in thermodynamics. Then the plan of instruction was designed. This plan indicates which instructional procedures and materials should be used to realize the instructional functions, derived from the learning theory. The evaluation design contained two control and three experimental courses. In discussing our main findings, we consider the generalizability of the procedures we followed in constructing the PAM and the instructional plan

Pilot

Several early issues of Pilot, a company newsletter produced by the Afro-American Life Insurance Company.Covers 1962-1964

Joint Pilot Design and Uplink Power Allocation in Multi-Cell Massive MIMO Systems

This paper considers pilot design to mitigate pilot contamination and provide good service for everyone in multi-cell Massive multiple input multiple output (MIMO) systems. Instead of modeling the pilot design as a combinatorial assignment problem, as in prior works, we express the pilot signals using a pilot basis and treat the associated power coefficients as continuous optimization variables. We compute a lower bound on the uplink capacity for Rayleigh fading channels with maximum ratio detection that applies with arbitrary pilot signals. We further formulate the max-min fairness problem under power budget constraints, with the pilot signals and data powers as optimization variables. Because this optimization problem is non-deterministic polynomial-time hard due to signomial constraints, we then propose an algorithm to obtain a local optimum with polynomial complexity. Our framework serves as a benchmark for pilot design in scenarios with either ideal or non-ideal hardware. Numerical results manifest that the proposed optimization algorithms are close to the optimal solution obtained by exhaustive search for different pilot assignments and the new pilot structure and optimization bring large gains over the state-of-the-art suboptimal pilot design.Comment: 16 pages, 8 figures. Accepted to publish at IEEE Transactions on Wireless Communication