SARS-CoV Antibody Prevalence in All Hong Kong Patient Contacts

A total of 1,068 asymptomatic close contacts of patients with severe acute respiratory (SARS) from the 2003 epidemic in Hong Kong were serologically tested, and 2 (0.19%) were positive for SARS coronavirus immunoglobulin G antibody. SARS rarely manifests as a subclinical infection, and at present, wild animal species are the only important natural reservoirs of the virus

Complex Coacervate-based Materials for Biomedicine

There has been increasing interest in complex coacervates for deriving and trans- porting biomaterials. Complex coacervates are a dense, polyelectrolyte-rich liq- uid that results from the electrostatic complexation of oppositely charged macroions. Coacervates have long been used as a strategy for encapsulation, par- ticularly in food and personal care products. More recent efforts have focused on the utility of this class of materials for the encapsulation of small molecules, pro- teins, RNA, DNA, and other biomaterials for applications ranging from sensing to biomedicine. Furthermore, coacervate-related materials have found utility in other areas of biomedicine, including cartilage mimics, tissue culture scaffolds, and adhesives for wet, biological environments. Here, we discuss the self- assembly of complex coacervate-based materials, current challenges in the intel- ligent design of these materials, and their utility applications in the broad field of biomedicine

Performance of the CMS muon trigger system in proton-proton collisions at √s = 13 TeV

The muon trigger system of the CMS experiment uses a combination of hardware and software to identify events containing a muon. During Run 2 (covering 2015-2018) the LHC achieved instantaneous luminosities as high as 2 × 10 cm s while delivering proton-proton collisions at √s = 13 TeV. The challenge for the trigger system of the CMS experiment is to reduce the registered event rate from about 40 MHz to about 1 kHz. Significant improvements important for the success of the CMS physics program have been made to the muon trigger system via improved muon reconstruction and identification algorithms since the end of Run 1 and throughout the Run 2 data-taking period. The new algorithms maintain the acceptance of the muon triggers at the same or even lower rate throughout the data-taking period despite the increasing number of additional proton-proton interactions in each LHC bunch crossing. In this paper, the algorithms used in 2015 and 2016 and their improvements throughout 2017 and 2018 are described. Measurements of the CMS muon trigger performance for this data-taking period are presented, including efficiencies, transverse momentum resolution, trigger rates, and the purity of the selected muon sample. This paper focuses on the single- and double-muon triggers with the lowest sustainable transverse momentum thresholds used by CMS. The efficiency is measured in a transverse momentum range from 8 to several hundred GeV

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

The Effects of Upper-Ocean Sea Temperatures and Salinity on the Intensity Change of Tropical Cyclones over the Western North Pacific and the South China Sea: An Observational Study

With increasing air and sea temperatures, the thermodynamic environments over the oceans are becoming more favourable for the development of intense tropical cyclones (TCs) with rapid intensification (RI). The South China coastal region consists of highly densely populated cities, especially over the Pearl River Delta (PRD) region. Intense TCs maintaining their strength or the RI of TCs close to the coastal region can present substantial forecasting challenges and have significant potential impacts on the coastal population. This study investigates the effect of sea-surface and sub-surface temperatures and salinity on the intensification of five TCs, namely Super Typhoon Hato in 2017, Super Typhoon Mangkhut in 2018, and Typhoon Talim, Super Typhoon Saola, and Severe Typhoon Koinu in 2023, which have significantly affected the South China coastal region and triggered high TC warning signals in Hong Kong in the past few years. This analysis utilised the Hong Kong Observatory’s TC best-track and intensity data, along with sea temperature and salinity profiles generated using the China Ocean ReAnalysis version 2 (CORA2) product from the National Marine Data and Information Service of China. It was found that high sea-surface temperatures (SST) of 30 °C or above for a depth of about 20 m, low sea-surface salinity (SSS) levels of 33.8 psu or below for a depth of at least 20 m, and strong salinity stratification of at least 0.6 psu per 100 m depth might offer useful hints for predicting the RI of TCs over the western North Pacific and the South China Sea (SCS) in operational forecasting, while noting other contributing environmental factors and synoptic flow patterns conducive to RI. This study represents the first documentation of sub-surface salinity’s impact on some intense TCs traversing the SCS during 2017–2023 based on an observational study. Our aim is to supplement operational techniques for forecasting RI with some quantitative guidance based on upper-level ocean observations of temperatures and salinity, on top of well-known but more rapidly changing dynamical factors like low-level convergence, weak vertical wind shear, and upper-level divergent outflow, as forecasted with numerical weather prediction models. This study will also encourage further research to refine the analysis of quantitative contributions from different RI factors and the identification of essential features for developing AI models as one way to improve the forecasting of TC RI before the TC makes landfall near the PRD, with due consideration given to the effect of freshwater river discharge from the Pearl River

Proceedings of The HKIE Geotechnical Division 41st Annual Seminar: Adapt to Challenges, Create to Thrive

This seminar proceedings contains articles on the various research ideas of the academic community and practitioners presented at The HKIE Geotechnical Division 41st Annual Seminar (GDAS2021). GDAS2021 was jointly organized by the Geotechnical Division, The Hong Kong Institution of Engineers, & The Hong Kong Geotechnical Society on July 18th May 2021. Seminar Title: The HKIE Geotechnical Division 41st Annual SeminarSeminar Acronym: GDAS2021Seminar Date: 18 May 2021Seminar Location: Online (Virtual Mode)Seminar Organizers: Geotechnical Division, The Hong Kong Institution of Engineers, & The Hong Kong Geotechnical Society