Caveolin-1 Plays a Crucial Role in Inhibiting Neuronal Differentiation of Neural Stem/Progenitor Cells via VEGF Signaling-Dependent Pathway

In the present study, we aim to elucidate the roles of caveolin-1(Cav-1), a 22 kDa protein in plasma membrane invaginations, in modulating neuronal differentiation of neural progenitor cells (NPCs). In the hippocampal dentate gyrus, we found that Cav-1 knockout mice revealed remarkably higher levels of vascular endothelial growth factor (VEGF) and the more abundant formation of newborn neurons than wild type mice. We then studied the potential mechanisms of Cav-1 in modulating VEGF signaling and neuronal differentiation in isolated cultured NPCs under normoxic and hypoxic conditions. Hypoxic embryonic rat NPCs were exposed to 1% O2 for 24 h and then switched to 21% O2 for 1, 3, 7 and 14 days whereas normoxic NPCs were continuously cultured with 21% O2. Compared with normoxic NPCs, hypoxic NPCs had down-regulated expression of Cav-1 and up-regulated VEGF expression and p44/42MAPK phosphorylation, and enhanced neuronal differentiation. We further studied the roles of Cav-1 in inhibiting neuronal differentiation by using Cav-1 scaffolding domain peptide and Cav-1-specific small interfering RNA. In both normoxic and hypoxic NPCs, Cav-1 peptide markedly down-regulated the expressions of VEGF and flk1, decreased the phosphorylations of p44/42MAPK, Akt and Stat3, and inhibited neuronal differentiation, whereas the knockdown of Cav-1 promoted the expression of VEGF, phosphorylations of p44/42MAPK, Akt and Stat3, and stimulated neuronal differentiation. Moreover, the enhanced phosphorylations of p44/42MAPK, Akt and Stat3, and neuronal differentiation were abolished by co-treatment of VEGF inhibitor V1. These results provide strong evidence to prove that Cav-1 can inhibit neuronal differentiation via down-regulations of VEGF, p44/42MAPK, Akt and Stat3 signaling pathways, and that VEGF signaling is a crucial target of Cav-1. The hypoxia-induced down-regulation of Cav-1 contributes to enhanced neuronal differentiation in NPCs

Workshop on Regulatory Preparedness for Innovation in Nanotechnology

This report summarises the presentations and discussions at the first NanoReg2 Workshop on Regulatory Preparedness for Innovation in Nanotechnology held in Ispra, Italy 5 to 6 October 2017 and attended by approximately 60 regulators, industry representatives and other stakeholders. NanoReg2 is a European Union (EU) Horizon 2020 project. At the workshop, Regulatory Preparedness was defined as the regulators' timely awareness of innovations and the regulator's actions to check whether present legislation covers all safety aspects of each innovation, including initiating revision of the legislation as appropriate. Regulatory Preparedness, and Safe-by-Design (SbD) jointly constitute the NanoReg2 Safe Innovation Approach (SIA) for developing innovative products based on nanotechnology. The workshop aimed to gather views and identify current practices in regulatory work on safety of innovative products, tools already in use or needed, and potential difficulties in implementing Regulatory Preparedness in the EU. Presentations addressed the current state of the safety of nanotechnology innovation. The viewpoints included the regulatory framework, the principles behind it and the agencies and authorities enforcing it; nanosafety research projects and their support system (e.g. the current EU Horizon 2020 Framework Programme); national nanosafety initiatives; and the development of tools, such as foresight tools and harmonised test guidelines by the OECD for data generation. The workshop served to generate ideas for achieving Regulatory Preparedness. The participants recognised that while regulators deal with the safety of innovations, only few systematic approaches to this work exist. Some innovative products may reach the market before their safety has been appropriately assessed, as illustrated by RAPEX, the Rapid Exchange of Information System. A continuous and proactive combination of interconnected activities was considered to be required for ensuring Regulatory Preparedness. Thus, anticipation, e.g. horizon scanning, was seen as important, as was communication between regulators, innovators (industry) and other stakeholders. Regulators need to become aware of innovative products under development to ensure that the legislation and methods for safety assessment are available and adequate. Innovators must be aware of regulatory requirements and their likely development. This mutual awareness helps to develop safe products and to avoid delays or other problems in obtaining market approval. Awareness can be achieved through communication, which requires trust, e.g. promoted via "trusted environments" for confidential inquiries and information sharing. Furthermore, regulators need early access to the existing information and data relevant to safety assessment of innovative products to provide timely guidance and advice to Industry as well as to develop strategies for dealing with uncertainty, e.g. by applying the precautionary principle. Regulatory Preparedness was discussed as part of the SIA, and a "road map" of actions was suggested and outlined. The workshop has thus contributed towards acceptance of implementing Regulatory Preparedness for innovation in nanotechnology through the participation of a variety of stakeholders. This paves the way for a better dialogue among stakeholders in a fast economic development cycle, where it is even more important to quickly identify emerging needs for new approaches to regulatory issues for innovationJRC.F.2-Consumer Products Safet

2017 HRS/EHRA/ECAS/APHRS/SOLAECE expert consensus statement on catheter and surgical ablation of atrial fibrillation: executive summary.

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2017 HRS/EHRA/ECAS/APHRS/SOLAECE expert consensus statement on catheter and surgical ablation of atrial fibrillation: executive summary.

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withdrawn 2017 hrs ehra ecas aphrs solaece expert consensus statement on catheter and surgical ablation of atrial fibrillation

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En vandring genom sprit och droger

I denna uppsatts görs ett försök att finna vilka faktorer som kan verka viktiga i en behandling mot ett drogberoende. Den behandlingsform som undersökts är AA:s tolvstegsprogram och dess gemenskap. Undersökningen har en kvalitativ ansatts där vår studie är uppbyggd på intervjuer med sju informanter som har erfarenhet av och arbetar i AA:s tolvstegsprogram och gemenskap, mot sitt drogberoende. Vår studie visar på att erkännandet av sitt beroende, gemenskapen och tron är de faktorer som tycks vara av störst betydelse för våra deltagares tillfrisknande

pOEtry pArk ExpAndEd

30 minAs a part of Sofie Berntsen and Karl Holmqvist’s exhibition in the Museum of Contemporary Art’s exhibition series «Rameau’s Nephews», this Academy Lecture dissolves into a performance, including the students Emma Brack, Amelia Beavis-Harrison, Kachun Lay, Ina Hagen, Daisuke Kosugi and professor Susanne M. Winterling from the Academy of Fine Art. Based on Ei Arakawa and Karl Holmqvist’s collaborative work «pOEtry pArk» (2010), this workshop plans and realizes the construction of an “active park” inside the Museum of Contemporary Art during the opening hour of Karl Holmqvist’s exhibition. The performance addresses how what we do is meant to expand on genres such as movement and the spoken word. What is a gesture? How can words shape the architectual space and our body movement? To what extent is the outdoor setting of a public park more free and open than that of a museum interior? Indoor and outdoor? Audience participation? What is part of a work and what is not? Karl Holmqvist includes a broad spectrum of mediums in his artistic practice, including performance, text, posters, installation and sculpture. He has participated in numerous important international exhibitions, including Manifesta 8 (2010), Murcia; PERFORMA05 and PERFORMA07, New York; as well as the ILLUMInations section of the 54th Venice Biennale (2011). By almost always working collaboratively, Ei Arakawa’s work “not only destabilizes the line between one artist and another, or between one’s own name and those of others; on a more fundamental level, it disturbs the separation between director and actor, between initiator and fellow traveler”. (Simon Baier, «THAT’S THE WAY WE DO IT») Selected performances and exhibitions: Gwangju Biennial, Korea (2014); Whitney Biennial, New York (2014); Carnegie International, Pittsburgh (2013) and the Georgian Pavilion at the 55th Venice Biennale (2013)

Publishing Manifestos : An International Anthology from Artists and Writers

"Independent publishing, art publishing, publishing as artistic practice, publishing counterculture, and the zine, DIY, and POD scenes have proliferated over the last two decades. So too have art book fairs, an increasingly important venue—or even medium—for art. Art publishing experienced a similar boom in the 1960s and 1970s, in response to the culture's "linguistic turn." Today, art publishing confronts the internet and the avalanche of language and images that it enables. The printed book offers artists both visibility and tangibility. Publishing Manifestos gathers texts by artists, authors, editors, publishers, designers, zinesters, and activists to explore this rapidly expanding terrain for art practice." -- Publisher's website