Graphene transistors are insensitive to pH changes in solution

We observe very small gate-voltage shifts in the transfer characteristic of as-prepared graphene field-effect transistors (GFETs) when the pH of the buffer is changed. This observation is in strong contrast to Si-based ion-sensitive FETs. The low gate-shift of a GFET can be further reduced if the graphene surface is covered with a hydrophobic fluorobenzene layer. If a thin Al-oxide layer is applied instead, the opposite happens. This suggests that clean graphene does not sense the chemical potential of protons. A GFET can therefore be used as a reference electrode in an aqueous electrolyte. Our finding sheds light on the large variety of pH-induced gate shifts that have been published for GFETs in the recent literature

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Search for pair-produced resonances decaying to quark pairs in proton-proton collisions at root s=13 TeV

A general search for the pair production of resonances, each decaying to two quarks, is reported. The search is conducted separately for heavier resonances (masses above 400 GeV), where each of the four final-state quarks generates a hadronic jet resulting in a four-jet signature, and for lighter resonances (masses between 80 and 400 GeV), where the pair of quarks from each resonance is collimated and reconstructed as a single jet resulting in a two-jet signature. In addition, a b-tagged selection is applied to target resonances with a bottom quark in the final state. The analysis uses data collected with the CMS detector at the CERN LHC, corresponding to an integrated luminosity of 35.9 fb(-1), from proton-proton collisions at a center-of-mass energy of 13 TeV. The mass spectra are analyzed for the presence of new resonances, and are found to be consistent with standard model expectations. The results are interpreted in the framework of R-parity-violating supersymmetry assuming the pair production of scalar top quarks decaying via the hadronic coupling lambda ''(312) or lambda ''(323) and upper limits on the cross section as a function of the top squark mass are set. These results probe a wider range of masses than previously explored at the LHC, and extend the top squark mass limits in the (t) over tilde -> qq' scenario.Peer reviewe

Multiparticle correlation studies in pPb collisions at sNN =8.16 TeV

The second- and third-order azimuthal anisotropy Fourier harmonics of charged particles produced in pPb collisions, at √sNN=8.16TeV, are studied over a wide range of event multiplicities. Multiparticle correlations are used to isolate global properties stemming from the collision overlap geometry. The second-order “elliptic” harmonic moment is obtained with high precision through four-, six-, and eight-particle correlations and, for the first time, the third-order “triangular” harmonic moment is studied using four-particle correlations. A sample of peripheral PbPb collisions at √sNN=5.02TeV that covers a similar range of event multiplicities as the pPb results is also analyzed. Model calculations of initial-state fluctuations in pPb and PbPb collisions can be directly compared to the high-precision experimental results. This work provides new insight into the fluctuation-driven origin of the v3 coefficients in pPb and PbPb collisions, and into the dominating overall collision geometry in PbPb collisions at the earliest stages of heavy ion interactions. ISSN:0556-2813 ISSN:1089-490

Measurement of ttˉ\hbox {t}{\bar{\hbox {t}}} normalised multi-differential cross sections in pp{\text {p}}{\text {p}} collisions at s=13 TeV\sqrt{s}=13\,{\text {TeV}} , and simultaneous determination of the strong coupling strength, top quark pole mass, and parton distribution functions

Normalised multi-differential cross sections for top quark pair ($$\hbox {t}{\bar{\hbox {t}}}$$) production are measured in proton-proton collisions at a centre-of-mass energy of 13$$\,{\text {TeV}}$$ using events containing two oppositely charged leptons. The analysed data were recorded with the CMS detector in 2016 and correspond to an integrated luminosity of $$35.9{\,{\text {fb}}^{-1}}$$. The double-differential $$\hbox {t}{\bar{\hbox {t}}}$$ cross section is measured as a function of the kinematic properties of the top quark and of the $$\hbox {t}{\bar{\hbox {t}}}$$ system at parton level in the full phase space. A triple-differential measurement is performed as a function of the invariant mass and rapidity of the $$\hbox {t}{\bar{\hbox {t}}}$$ system and the multiplicity of additional jets at particle level. The data are compared to predictions of Monte Carlo event generators that complement next-to-leading-order (NLO) quantum chromodynamics (QCD) calculations with parton showers. Together with a fixed-order NLO QCD calculation, the triple-differential measurement is used to extract values of the strong coupling strength $$\alpha _{S}$$ and the top quark pole mass ($$m_{{\text {t}}}^{{\text {pole}}}$$) using several sets of parton distribution functions (PDFs). The measurement of $$m_{{\text {t}}}^{{\text {pole}}}$$ exploits the sensitivity of the $$\hbox {t}{\bar{\hbox {t}}}$$ invariant mass distribution to $$m_{{\text {t}}}^{{\text {pole}}}$$ near the production threshold. Furthermore, a simultaneous fit of the PDFs, $$\alpha _{S}$$, and $$m_{{\text {t}}}^{{\text {pole}}}$$ is performed at NLO, demonstrating that the new data have significant impact on the gluon PDF, and at the same time allow an accurate determination of $$\alpha _{S}$$ and $$m_{{\text {t}}}^{{\text {pole}}}$$. The values $$\alpha _{S}(m_{{\text {Z}}}) = 0.1135{}^{+0.0021}_{-0.0017}$$ and $$m_{{\text {t}}}^{{\text {pole}}} = 170.5 \pm 0.8 \,{\text {GeV}}$$ are extracted, which account for experimental and theoretical uncertainties, the latter being estimated from NLO scale variations. Possible effects from Coulomb and soft-gluon resummation near the $$\hbox {t}{\bar{\hbox {t}}}$$ production threshold are neglected in these parameter extractions. A rough estimate of these effects indicates an expected correction of $$m_{{\text {t}}}^{{\text {pole}}}$$ of the order of $$+1 \,{\text {GeV}}$$, which can be regarded as additional theoretical uncertainty in the current $$m_{{\text {t}}}^{{\text {pole}}}$$ extraction

Observation of nuclear modifications in W± boson production in pPb collisions at sNN=8.16TeV

The production of W± bosons is studied in proton-lead (pPb) collisions at a nucleon-nucleon centre-of-mass energy of sNN=8.16TeV. Measurements are performed in the W±→μ±νμ channel using a data sample corresponding to an integrated luminosity of 173.4±6.1nb−1, collected by the CMS Collaboration at the LHC. The number of positively and negatively charged W bosons is determined separately in the muon pseudorapidity region in the laboratory frame |ηlabμ|25GeV/c. The W± boson differential cross sections, muon charge asymmetry, and the ratios of W± boson yields for the proton-going over the Pb-going beam directions are reported as a function of the muon pseudorapidity in the nucleon-nucleon centre-of-mass frame. The measurements are compared to the predictions from theoretical calculations based on parton distribution functions (PDFs) at next-to-leading-order. The results favour PDF calculations that include nuclear modifications and provide constraints on the nuclear PDF global fits

Measurement of the cross section for tt‾ \mathrm{t}\overline{\mathrm{t}} production with additional jets and b jets in pp collisions at s \sqrt{s} = 13 TeV

Measurements of the cross section for the production of top quark pairs in association with a pair of jets from bottom quarks $$\left({\sigma}_{\mathrm{t}\overline{\mathrm{t}}\mathrm{b}\overline{\mathrm{b}}}\right)$$and in association with a pair of jets from quarks of any flavor or gluons $$\left({\sigma}_{\mathrm{t}\overline{\mathrm{t}}\mathrm{jj}}\right)$$and their ratio are presented. The data were collected in proton-proton collisions at a center-of-mass energy of 13 TeV by the CMS experiment at the LHC in 2016 and correspond to an integrated luminosity of 35.9 fb−1. The measurements are performed in a fiducial phase space and extrapolated to the full phase space, separately for the dilepton and lepton+jets channels, where lepton corresponds to either an electron or a muon. The results of the measurements in the fiducial phase space for the dilepton and lepton+jets channels, respectively, are $${\sigma}_{\mathrm{t}\overline{\mathrm{t}}\mathrm{jj}}$$= 2.36±0.02 (stat)±0.20 (syst) pb and 31.0±0.2 (stat)±2.9 (syst) pb, and for the cross section ratio 0.017 ± 0.001 (stat) ± 0.001 (syst) and 0.020 ± 0.001 (stat) ± 0.001 (syst). The values of $${\sigma}_{\mathrm{t}\overline{\mathrm{t}}\mathrm{b}\overline{\mathrm{b}}}$$are determined from the product of the $${\sigma}_{\mathrm{t}\overline{\mathrm{t}}\mathrm{jj}}$$and the cross section ratio, obtaining, respectively, 0.040±0.002 (stat)±0.005 (syst) pb and 0.62±0.03 (stat)±0.07 (syst) pb. These measurements are the most precise to date and are consistent, within the uncertainties, with the standard model expectations obtained using a matrix element calculation at next-to-leading order in quantum chromodynamics matched to a parton shower

Observation of the Λb0→J/ψΛφ decay in proton-proton collisions at s=13TeV

The observation of the Λb0→J/ψΛφ decay is reported using proton-proton collision data collected at s=13TeV by the CMS experiment at the LHC in 2018, corresponding to an integrated luminosity of 60fb−1. The ratio of the branching fractions B(Λb0→J/ψΛφ)/B(Λb0→ψ(2S)Λ) is measured to be (8.26±0.90(stat)±0.68(syst)±0.11(B))×10−2, where the first uncertainty is statistical, the second is systematic, and the last uncertainty reflects the uncertainties in the world-average branching fractions of φ and ψ(2S) decays to the reconstructed final states

Search for single production of vector-like quarks decaying to a top quark and a W boson in proton–proton collisions at s=13TeV

A search is presented for the single production of vector-like quarks in proton-proton collisions at s = 13 TeV . The data, corresponding to an integrated luminosity of 35.9 fb - 1 , were recorded with the CMS experiment at the LHC. The analysis focuses on the vector-like quark decay into a top quark and a W boson, with one muon or electron in the final state. The mass of the vector-like quark candidate is reconstructed from hadronic jets, the lepton, and the missing transverse momentum. Methods for the identification of b quarks and of highly Lorentz boosted hadronically decaying top quarks and W bosons are exploited in this search. No significant deviation from the standard model background expectation is observed. Exclusion limits at 95% confidence level are set on the product of the production cross section and branching fraction as a function of the vector-like quark mass, which range from 0.3 to 0.03 pb for vector-like quark masses of 700 to 2000 GeV . Mass exclusion limits up to 1660 GeV are obtained, depending on the vector-like quark type, coupling, and decay width. These represent the most stringent exclusion limits for the single production of vector-like quarks in this channel

Measurement of charged pion, kaon, and proton production in proton-proton collisions at root s=13 TeV

Transverse momentum spectra of charged pions, kaons, and protons are measured in proton-proton collisions at √s = 13 TeV with the CMS detector at the LHC. The particles, identified via their energy loss in the silicon tracker, are measured in the transverse momentum range of pT ∼ 0.1-1.7 GeV/c and rapidities /y/ < 1. The pT spectra and integrated yields are compared to previous results at smaller √s and to predictions of Monte Carlo event generators. The average pT increases with particle mass and charged particle multiplicity of the event. Comparisons with previous CMS results at √s = 0.9, 2.76, and 7 TeV show that the average pT and the ratios of hadron yields feature very similar dependences on the particle multiplicity in the event, independently of the center-of-mass energy of the pp collision