Role of Embryonic and Maternal Genotype on Prenatal Survival and Foetal Growth in Rabbit

[EN] The aim of this work was to evaluate the influence of maternal and embryonic genotype on prenatal survival and foetal growth during pregnancy. Embryos were recovered at 48 h of gestation from two different donor lines (R = 46 and A = 40) and transferred to nulliparous recipient does (26 R and 24 A). Each recipient doe received six embryos into one oviduct from line R, and six embryos form line A into the other. Laparoscopy was performed at Day 14 to determine implantation rate. Recipient females were slaughter at Days 14, 24 and 30 (12, 24, and 14, respectively) to determine the number of live foetuses and the weight of live foetuses, foetal placenta and maternal placenta. A transcriptome analysis was performed to search for differences between foetal placentas at Days 14 and 24 of development. Prenatal survival at Days 14, and 24 was affected by embryonic genotype and determined by maternal genotype at Day 30. Foetal weight at Day 14 was influenced by both genotypes, being the weight higher for group A/A (0.29 0.01 g vs 0.19 0.01 g, for group R/R). However, both genotypes were determinant for foetal placenta weight at Day 24, while those genotypes affected maternal placenta weight at Day 30. Nevertheless, no differences in foetal placenta at transcriptome level and progesterone and IGF-I plasma levels in recipient does were found. In conclusion, results indicate that the influence of embryo and maternal genotype on the prenatal survival and growth seems to be changing over gestation.Naturil Alfonso, C.; Marco Jiménez, F.; Jiménez Trigos, ME.; Saenz De Juano Ribes, MDLD.; Viudes De Castro, MP.; Lavara García, R.; Vicente Antón, JS. (2015). Role of Embryonic and Maternal Genotype on Prenatal Survival and Foetal Growth in Rabbit. Reproduction in Domestic Animals. (50):312-320. doi:10.1111/rda.12493S3123205

Secretion of MCP-1 and other paracrine factors in a novel tumor-bone coculture model

BackgroundThe bone-tumor microenvironment encompasses unique interactions between the normal cells of the bone and marrow cavity and the malignant cells from a primary or metastasized cancer. A multitude of paracrine factors within this microenvironment such as the growth factor, TGF-beta, and the chemokine, MCP-1, are secreted by many of these cell types. These factors can act in concert to modulate normal and malignant cell proliferation, malignant cell migration and invasion and, often, mediate bone cancer pain. Although many valuable in vitro and in vivo models exist, identifying the relevant paracrine factors and deciphering their interactions is still a challenge. The aim of our study is to test an ex vivo coculture model that will allow monitoring of the expression, release and regulation of paracrine factors during interactions of an intact femur explant and tumor cells.MethodsIntact or marrow-depleted neonatal mouse femurs and select murine and human sarcoma or carcinoma cell lines were incubated singly or in coculture in specialized well plates. Viability of the bone and cells was determined by immunohistochemical stains, microscopy and marrow cytopreps. Secretion and mRNA expression of paracrine factors was quantitated by ELISA and real-time RT-PCR.ResultsCompartments of the bone were optimally viable for up to 48 h in culture and tumor cells for up to 4 days. Bone was the major contributor of TGF-beta and MMP2 whereas both bone and sarcoma cells secreted the chemokine MCP-1 in cocultures. Synergistic interaction between the femur and sarcoma resulted in enhanced MCP-1 secretion and expression in cocultures and was dependent on the presence of the hematopoietic component of the bone as well as other bone cells. In contrast, coculturing with breast carcinoma cells resulted in reduction of TGF-beta and MCP-1 secretion from the bone.ConclusionThese studies illustrate the feasibility of this model to examine paracrine interactions between intact bone and tumor cells. Further study of unique regulation of MCP-1 secretion and signaling between these cell types in different types of cancer will be possible using this simulated microenvironment

Computational approaches to understanding reaction outcomes of organic processes in ionic liquids

This review considers how various computational methods have been applied to explain the changes in reaction outcome on moving from a molecular to an ionic liquid solvent. Initially, different conceptual approaches to modelling ionic liquids are discussed, followed by a consideration of the limitations and constraints of these approaches. A series of case studies demonstrating the utility of computational approaches to explain processes in ionic liquids are considered; some of these address the solubility of species in ionic liquids while others examine classes of reaction where the outcome in ionic liquids can be explained through the application of computational approaches. Overall, the utility of computational methods to explain, and potentially predict, the effect of ionic liquids on reaction outcome is demonstrated

Barbarians at the British Museum: Anglo-Saxon Art, Race and Religion

A critical historiographical overview of art historical approaches to early medieval material culture, with a focus on the British Museum collections and their connections to religion

Political Islam and European Foreign Policy: Perspectives from Muslim Democrats of the Mediterranean. CEPS Paperbacks. November 2007

The time is ripe for the European Union, its institutions and member states to undertake an explicit review of its current policy of ‘benign neglect’ towards the broad collection of ‘Muslim democrat’ parties in the Mediterranean Arab states. The group of experts assembled to produce this new book adduces mounting evidence that this policy may lead to unintended consequences, such as the reinforcement of anti-democratic regimes and radical Islamism. Their arguments favour a broad inclusion of Muslim democrats in EU initiatives aiming at the reform of governance and the development of civil society, without extending to them any singular, exclusive or unsolicited privileges

Islamic Radicalization: The Challenge for Euro-Mediterranean Relations. CEPS Paperbacks. June 2009

On June 4th, Obama will deliver his long-anticipated speech in Cairo, which is expected to convey a reconciliatory message to Arabs and Muslims across the world. As both the US and the EU are trying to reset their policies in the Middle East, the challenge of how to deal with the most important emerging actors in the region – Islamists – remains a matter of great controversy. In this book, Arab and European authors ask whether the EU's current policies are contributing to the radicalisation of Islamist movements in the Middle East and North Africa, and argue in favour of an EU policy based on inclusion