MIF Participates in Toxoplasma gondii-Induced Pathology Following Oral Infection

BACKGROUND: Macrophage migration inhibitory factor (MIF) is essential for controlling parasite burden and survival in a model of systemic Toxoplasma gondii infection. Peroral T. gondii infection induces small intestine necrosis and death in susceptible hosts, and in many aspects resembles inflammatory bowel disease (IBD). Considering the critical role of MIF in the pathogenesis of IBD, we hypothesized that MIF participates in the inflammatory response induced by oral infection with T. gondii. METHODOLOGY/PRINCIPAL FINDINGS: Mif deficient (Mif(-/-)) and wild-type mice in the C57Bl/6 background were orally infected with T. gondii strain ME49. Mif(-/-) mice had reduced lethality, ileal inflammation and tissue damage despite of an increased intestinal parasite load compared to wt mice. Lack of MIF caused a reduction of TNF-α, IL-12, IFN-γ and IL-23 and an increased expression of IL-22 in ileal mucosa. Moreover, suppressed pro-inflammatory responses at the ileal mucosa observed in Mif(-/-) mice was not due to upregulation of IL-4, IL-10 or TGF-β. MIF also affected the expression of matrix metalloproteinase-9 (MMP-9) but not MMP-2 in the intestine of infected mice. Signs of systemic inflammation including the increased concentrations of inflammatory cytokines in the plasma and liver damage were less pronounced in Mif(-/-) mice compared to wild-type mice. CONCLUSION/SIGNIFICANCE: In conclusion, our data suggested that in susceptible hosts MIF controls T. gondii infection with the cost of increasing local and systemic inflammation, tissue damage and death

Numerische Simulation nichtlinearer transienter Wellengruppen Zwischenbericht zum Forschungsvorhaben

SIGLEAvailable from TIB Hannover: F03B153 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDeutsche Forschungsgemeinschaft (DFG), Bonn (Germany)DEGerman

Extreme waves in sea states crossing an oblique current

Laboratory experiments have been carried out in the directional wave tank at Marintek (Norway) to study the nonlinear dynamics of surface gravity waves and the occurrence of extreme events, when the wave field traverses obliquely an ambient current. A condition of partial opposition has been considered. Tests on regular waves have shown that the current can trigger the formation of large amplitude waves. In random wave fields, however, this only results in a weak deviation from the statistical properties observed in absence of a current

Experimental evidence of the modulation of a plane wave to oblique perturbations and generation of rogue waves in finite water depth

We present a laboratory experiment in a large directional wave basin to discuss the instability of a plane wave to oblique side band perturbations in finite water depth. Experimental observations, with the support of numerical simulations, confirm that a carrier wave becomes modulationally unstable even for relative water depths k0h < 1.36 (with k the wavenumber of the plane wave and h the water depth), when it is perturbed by appropriate oblique disturbances. Results corroborate that the underlying mechanism is still a plausible explanation for the generation of rogue waves in finite water depth

Thigh-length compression stockings and DVT after stroke

Controversy exists as to whether neoadjuvant chemotherapy improves survival in patients with invasive bladder cancer, despite randomised controlled trials of more than 3000 patients. We undertook a systematic review and meta-analysis to assess the effect of such treatment on survival in patients with this disease