Effectiveness of accessibility information on hotels’ websites: a quasi-experimental design

People with disabilities is a large potential tourism market. Research shows, however, information available on lodging facility’s websites is a major barrier for people with mobility disability. Booking hotel rooms online can be a challenge because accessibility information online is not detailed and guests using wheelchairs often have to call the property for more information. To provide a more inclusive lodging environment, the study utilized a quasi-experimental design to test the effectiveness of AccInfo, a set of detailed accessibility information relevant for people with spinal cord injury. Participants’ evaluation of accessibility information, their satisfaction with the hotel\u27s website information, and their intention to book the hotel online were measured. Results suggest that AccInfo was effective in increasing the satisfaction of guests with spinal cord injury with hotel website information and their intention to book the hotel online without having to call the property

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM

Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics of fluorescence, various techniques have been developed that enable the visualization and analysis of complex dynamic events in cells, organelles, and sub-organelle components within the biological specimen. The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Forster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. Subsequently, these advanced microscopy techniques are introduced in more detail, with a description of how these techniques are performed, what needs to be considered, and what practical advantages they can bring to cell biological research

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Developing a Facilitators Scale in the Context of Travel: ReTRIP

Objective: To develop a scale for measuring factors that facilitate participation of people with spinal cord injury (SCI) in travel-related activities: Removing Travel Restrictions Influencing Participation (ReTRIP). Design: A mixed-method approach where in the qualitative phase, items were developed and written based on results of interviewers with different stakeholder groups and in the quantitative phase, survey data were collected to examine the psychometric properties of the scale. Setting: Home, work, and community settings. Participants: People living with SCI, caregivers or family members, therapists, travel professionals (N=333). Interventions: None. Main Outcome Measures: An 11-item ReTRIP scale that measures the facilitators that enhance the travel participation of people with SCI. Results: In the qualitative phase of the study, 5 categories of travel facilitators were identified based on semistructured in-depth interviews with 83 respondents from 4 stakeholder groups. Initial items of the ReTRIP scale were written based on the travel facilitators identified. Items in the scale were then revised based on results of cognitive interviews and an expert panel review. In the quantitative phase, a total of 250 patients enrolled in a Spinal Cord Injury Model System were systematically selected to report their experience with each travel facilitator. Item-response theory–based Rasch analysis revealed that the 11-item ReTRIP has acceptable psychometric properties, containing 2 main dimensions: industry-oriented facilitators (6 items) and self-oriented facilitators (5 items). Conclusions: The 11-item ReTRIP scale demonstrates promising psychometric properties, allowing researchers and clinicians to potentially use self-reported environmental factors that are beneficial for people’s participation in travel after SCI to properly design client-centered interventions. Future studies using a larger sample are needed to validate the scale

Resident Support for Tourism Development in Rural Midwestern (USA) Communities: Perceived Tourism Impacts and Community Quality of Life Perspective

Local residents play an important role in the process of sustainable development in tourism. Resident support for tourism development contributes to the health of tourism industry and successful community development. Therefore, it is in the best interest of local residents, the tourism industry, and tourists, that residents have a positive outlook on and positive experiences with tourism development. In order to understand resident support for tourism development from tourism impacts and community quality of life perspective within the rural communities of Orange County, Indiana, USA, this study has examined a proposed structural model which incorporates eight latent variables: (a) six types of positive and negative tourism impacts serve as exogenous latent variables; (b) tourism-related community quality of life (TCQOL) is proposed as the mediating variable; and (c) resident support for tourism development is the ultimate dependent variable. The results show that both sociocultural and environmental benefits contribute to the host community’s living experience. Economic and sociocultural benefits, negative sociocultural and environmental impacts, and TCQOL influence resident support for tourism development. This study identified specific tourism impacts that affect TCQOL and resident support for local tourism development. This study affirms that community quality of life (QOL) serves an effective predictor of support for tourism development