Empathy in its Entirety

Reymann critically analyzes three novels through the lens of empathy and then applies her critical analysis and observations to her experiences as a person diagnosed with Asperger’s Syndrome

The textual dimension "Involved-Informational": A corpus-based study

In the study "The Textual Dimension Involved-Informational", algorithms and their application to corpora of the English Language will be presented. A ground-breaking study predestined to be exhaustively transferred to computer-aided linguistics is presented in Douglas Biber's "Variation across speech and writing" (1988) which describes a way to establish a general typology of English texts. In his study, Biber derives a so-called multi-dimensional (MD) approach to typology based on the frequency of specific grammatical phenomena. The study at hand will focus on the dimension "Involved-Informational". The first chapter deals with the establishing of a completely automatic and modularized computer system written in the programming language PERL, that is able to process any given 'raw' text and produce CSV (comma separated values) files of feature occurrences of the 30 features listed by Biber (1989: 8). The second chapter describes its application on text corpora of English, such as the commonly used LOB/FLOB and BROWN/FROWN corpus pairs as representatives of written English, and the less commonly analyzed corpora of spoken English SEC and COLT

The Leader as Servant: Followership to Leadership

A paradigm change has been occurring in leadership[p theory over the last 40 years since Servant Leadership was introduced in the 1970\u27s by Robert Greenleaf. For many years academia looked away from including this theory as foundational. In recent years the theory has gained strong attention in academic journals, textbooks and course instruction. Pivotal to the underpinnings of this theory is the concept of the leader as a developer of his or her followers. The challenge for any leader is how to mentor, develop and coach his or her followers through leadership practices that truly develop others. The practice of this involves these behaviors: empowerment, ethical actions, mentoring others, community-wide thinking, trust, humility, and stewardship.https://fuse.franklin.edu/ss2018/1073/thumbnail.jp

Unlocking the Question

Educators are constantly seeking ways to establish equity inside and outside of the classroom. This presentation explores ways in which an inquisitive mindset can make steps toward achieving this goal, and how questioning is essential for creating the optimal learning environment

Transcriptome profiling of human hepatocytes treated with Aroclor 1254 reveals transcription factor regulatory networks and clusters of regulated genes

BACKGROUND: Aroclor 1254 is a well-known hepatotoxin and consists of a complex mixture of polychlorinated biphenyls (PCBs), some of which have the ability to activate the aryl hydrocarbon receptor (AhR) and other transcription factors (TFs). Altered transcription factor expression enables activation of promoters of many genes, thereby inducing a regulatory gene network. In the past, computational approaches were not applied to understand the combinatorial interplay of TFs acting in concert after treatment of human hepatocyte cultures with Aroclor 1254. We were particularly interested in interrogating promoters for transcription factor binding sites of regulated genes. RESULTS: Here, we present a framework for studying a gene regulatory network and the large-scale regulation of transcription on the level of chromatin structure. For that purpose, we employed cDNA and oligomicroarrays to investigate transcript signatures in human hepatocyte cultures treated with Aroclor 1254 and found 910 genes to be regulated, 52 of which code for TFs and 47 of which are involved in cell cycle and apoptosis. We identified regulatory elements proximal to AhR binding sites, and this included recognition sites for the transcription factors ETS, SP1, CREB, EGR, NF-kB, NKXH, and ZBP. Notably, ECAT and TBP binding sites were identified for Aroclor 1254-induced and E2F, MAZ, HOX, and WHZ for Aroclor 1254-repressed genes. We further examined the chromosomal distribution of regulated genes and observed a statistically significant high number of gene pairs within a distance of 200 kb. Genes regulated by Aroclor 1254, are much closer located to each other than genes distributed randomly all over the genome. 37 regulated gene pairs are even found to be directly neighbored. Within these directly neighbored gene pairs, not all genes were bona fide targets for AhR (primary effect). Upon further analyses many were targets for other transcription factors whose expression was regulated by Aroclor 1254 (secondary effect). CONCLUSION: We observed coordinate events in transcript regulation upon treatment of human hepatocytes with Aroclor 1254 and identified a regulatory gene network of different TFs acting in concert. We determined molecular rules for transcriptional regulation to explain, in part, the pleiotropic effect seen in animals and humans upon exposure to Aroclor 1254

Topoisomerase II inhibition involves characteristic chromosomal expression patterns

<p>Abstract</p> <p>Background</p> <p>The phenomenon of co-localization of transcriptionally upregulated genes showing similar expression levels is known across all eukaryotic genomes. We recently mapped the Aroclor 1254-regulated transcriptome back onto the genome and provided evidence for the statistically significant co-localization of regulated genes. They did, however, not always show similar expression levels, and many of the regulated genes were, in fact, repressed.</p> <p>Results</p> <p>In this study, we were able to reproduce this observation with microarray data stemming from 1) human hepatocytes treated with the gyrase and potential topoisomerase II inhibitor trovafloxacin, 2) human hepatocytes treated with the topoisomerase II inhibitor doxorubicin and 3) mouse lymphoma cells treated with the topoisomerase II inhibitor etoposide. We found statistically significant co-localization of regulated gene pairs – induced and repressed – within the window size of 0–100 kbp. Notably, by using microarray data stemming from lung tissue of a mouse transgenic line overexpressing the transcription factor c-myc, which served as a negative control, we found regulated genes to be located with regard to each other nearly in the same way as genes distributed randomly all over the genome (0–100 kbp).</p> <p>Conclusion</p> <p>We suggest topoisomerase II inhibition by Aroclor 1254, trovafloxacin, doxorubicin, and etoposide to be responsible for significant co-localization of regulated genes through the inability of the stabilized enzyme complexes to religate DNA. Within the permanently opened chromatin domains, neighbored genes might be allowed to be regulated. Overexpression of c-myc, however, does not inhibit topoisomerase II activity. Consequently, the enzyme is able to perform its normal function of transiently breaking and rejoining the DNA double strand. As a result, exclusively target genes are regulated.</p

A Site and Stormwater Management Plan for a Boy Scout Council Service Center in Worcester, MA

This project aims to produce a site and stormwater management plan for the development of

Innovative technologies of extruded foods with alcoprotective action on the basis of fish raw

У статті наведений аналіз динаміки споживчих вподобань екструдованої снекової продукції на основі рибної сировини та обгрунтована доцільність створення харчових композицій з крупок зернових та картопляних пластівців, збагачених рибними порошками, з регулюємим хімічним складом та біологічною цінністю. З точки зору хімічних та біохімічних процесів описано властивості гліцину та бурштинової кислоти, як речовин, що нейтралізують токсичну дію ацетальдегіду в організмі людини під час вживання слабоалкогольних напоїв. Обгрунтована перспективність виробництва екструдованої продукції з компонентами, що перешкоджають руйнуванню клітин печінки та мозку у разі споживання слабоалкогольних напоїв. Проведена органолептична оцінка розроблених екструдованих продуктів, за результатами якої обгрунтована перспективність використання рибних порошків для виробництва екструдованих продуктів.The article analyzes the dynamics of consumer preferences of extruded snack foods on the basis of fish raw materials and substantiates the expediency of creating food compositions from grains of cereals and potato flakes enriched with fish powders, with regulated chemical composition and biological value. From the point of view of chemical and biochemical processes, the properties of glycine and amber acid are described as substances that neutralize the toxic effect of acetaldehyde in the human body during the use of low-alcohol beverages. The prospect of production of extruded products with components that prevent the destruction of liver and brain cells in the case of low-alcohol beverages is substantiated. The organoleptic evaluation of extruded products developed, the results of which proved the prospect of using fish powders for the production of extruded products. In the given work the technology of innovative food product - with functional alkoprotektornymi properties was presented. It is assumed that this product will expand the range of snack snacks to low-alcohol beverages and will be a reliable protection against the harmful effects of alcohol and its metabolism products in the human bod