Glial activation involvement in neuronal death by Japanese encephalitis virus infection

Japanese encephalitis is characterized by profound neuronal destruction/dysfunction and concomitant microgliosis/astrogliosis. Although substantial activation of glia is observed in Japanese encephalitis virus (JEV)-induced Japanese encephalitis, the inflammatory responses and consequences of astrocytes and microglial activation after JEV infection are not fully understood. In this study, infection of cultured neurons/glia with JEV caused neuronal death and glial activation, as evidenced by morphological transformation, increased cell proliferation and elevated tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6 and RANTES (regulated upon activation, normal T-cell expressed and secreted) production. Replication-competent JEV caused all glial responses and neurotoxicity. However, replication-incompetent JEV lost these abilities, except for the ability to change microglial morphology. The bystander damage caused by activated glia also contributed to JEV-associated neurotoxicity. Microglia underwent morphological changes, increased cell proliferation and elevated TNF-alpha, IL-1 beta, IL-6 and RANTES expression in response to JEV infection. In contrast, IL-6 and RANTES expression, but no apparent morphological changes, proliferation or TNF-alpha/IL-1 beta expression, was demonstrated in JEV-infected astrocytes. Supernatants of JEV-infected microglia, but not JEV-infected astrocytes, induced glial activation and triggered neuronal death. Antibody neutralization studies revealed that TNF-alpha and IL-1 beta, but not RANTES or IL-6, released by activated microglia appeared to play roles in JEV-associated neurotoxicity. In conclusion, following JEV infection, neuronal death was accompanied by concomitant microgliosis and astrogliosis, and neurotoxic mediators released by JEV-activated microglia, rather than by JEV-activated astrocytes, had the ability to amplify the microglial response and cause neuronal death

PT-symmetric Solutions of Schrodinger Equation with position-dependent mass via Point Canonical Transformation

PT-symmetric solutions of Schrodinger equation are obtained for the Scarf and generalized harmonic oscillator potentials with the position-dependent mass. A general point canonical transformation is applied by using a free parameter. Three different forms of mass distributions are used. A set of the energy eigenvalues of the bound states and corresponding wave functions for target potentials are obtained as a function of the free parameter.Comment: 13 page

Correlation dynamics of three spin under a classical dephasing environment

By starting from the stochastic Hamiltonian of the three correlated spins and modeling their frequency fluctuations as caused by dephasing noisy environments described by Ornstein-Uhlenbeck processes, we study the dynamics of quantum correlations, including entanglement and quantum discord. We prepared initially our open system with Greenberger-Horne-Zeilinger or W state and present the exact solutions for evolution dynamics of entanglement and quantum discord between three spins under both Markovian and non-Markovian regime of this classical noise. By comparison the dynamics of entanglement with that of quantum discord we find that entanglement can be more robust than quantum discord against this noise. It is shown that by considering non-Markovian extensions the survival time of correlations prolong.Comment: 13 pages, 4 figure

Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set

We report a measurement of the bottom-strange meson mixing phase \beta_s using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays in which the quark-flavor content of the bottom-strange meson is identified at production. This measurement uses the full data set of proton-antiproton collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity. We report confidence regions in the two-dimensional space of \beta_s and the B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2, -1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in agreement with the standard model expectation. Assuming the standard model value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +- 0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +- 0.009 (syst) ps, which are consistent and competitive with determinations by other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012

Constitutive behavior of an AA4032 piston alloy with Cu and Er additions upon high temperature compressive deformation

The Research and Researchers for Industry (RRi); King Mongkut’s University of Technology Thonbur

Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome

To investigate large structural clonal mosaicism of chromosome X, we analysed the SNP microarray intensity data of 38,303 women from cancer genome-wide association studies (20,878 cases and 17,425 controls) and detected 124 mosaic X events42Mb in 97 (0.25%) women. Here we show rates for X-chromosome mosaicism are four times higher than mean autosomal rates; X mosaic events more often include the entire chromosome and participants with X events more likely harbour autosomal mosaic events. X mosaicism frequency increases with age (0.11% in 50-year olds; 0.45% in 75-year olds), as reported for Y and autosomes. Methylation array analyses of 33 women with X mosaicism indicate events preferentially involve the inactive X chromosome. Our results provide further evidence that the sex chromosomes undergo mosaic events more frequently than autosomes, which could have implications for understanding the underlying mechanisms of mosaic events and their possible contribution to risk for chronic diseases

INVESTIGATION OF CHEMICAL QUALITY OF SUGARCANE (SACCHARUM OFFICINARUM L.) WINE DURING FERMENTATION BY SACCHAROMYCES CEREVISIAE

The purpose of this study was to examine the changes in the contents of sugar ethanol, organic acids, volatile compounds, pH and acidity in sugarcane wine during fermentation using Saccharomyces cerevisiae and the consumer preference test was also conducted to investigate the optimal fermentation. According to the results, the maximal population of S. cerevisiae reached to 8.12 log cfu/mL at the sixth day of :fermentation. The maximum ethanol content, 15.16%, was obtained at the ninth day, while the sucrose and glucose concentrations were almost exhausted at the SOW time. As for organic acids, citric acid was detected as the most abundant one at the second day of fermentation, followed by lactic and succinic acids. The volatile compounds were analyzed by SPME/GC-MS, and the concentrations of alcohols, esters and acids were all highest in sugarcane wine at the sixth day of fermentation. The results' have demonstrated that sugarcane wine fermentation with S. cerevisiae at the sixth day is enough to reach the highest aroma quality as indicated by the highest ester/alcohols in the wine and the most abundant amount of aroma-related compounds. The overall preference of consumer test also supports the above result with the sugarcane wine fermented at day 6 having highest scores