26 research outputs found
Effect of dietary n-6 to n-3 polyunsaturated fatty acid ratio on prostaglandin plasma levels and genes expression peroxisome proliferator-activated receptor (PPAR) in pregnant Sprague Dawley rats
The peroxisome proliferator-activated receptors (PPARs) are a family of nuclear transcription factors thought to act as receptors for polyunsaturated fatty acids and to reduce production of series 2 prostaglandins (PG). The objective of this study was to investigate the effect of different ratio n-6:n-3 on the PPAR expression of rats endometrial tissue. The findings obtained from this study showed significant induction of PPARδ mRNA levels in endomatral cells treatment 1:1 group by 1.38 fold compared with the PPARδ mRNA levels in endomatral cells treatment 30:1 group. This induction was due to the cellular demands for prostaglandin were high in the endometrial cells when cells were treated with high ratio n6:n3 on 30:1 group, thus, resulting in an increase in both prostaglandin PGE2 and PGF2α production by induction of PPARδ genes. On the other hand, treatment 1:1 group and control group of endometrial cells did not show any significant changes in mRNA level of PPARδ, compared with treatment ratio n6:n3 on 6:1 group and treatment high ratio n6:n3 on 30:1 group of the endometrial cells. These findings show that inhibition of uterine PGF2α synthesis by n-3 fatty acids may depend on the amount of n-6 fatty acids reaching the target tissue. In conclusion, PPARδ function in the response of rat endometrium to long chain n-6:n3 polyunsaturated fatty acids.Key words: Polyunsaturated fatty acid, gene expression, peroxisome proliferator-activated receptor, prostaglandin, pregnancy rat
Effect of n-3 and n-6 fatty acid supplementation on fetal, gestation and parturition in pregnant Sprague Dawley rats
The aim of this study was to evaluate the effect of different ratio of n-6:n-3 on gestation and parturition as well as to determine the hormone concentration on plasma of the pregnant rats. At the end of the trial period, blood plasma was collected and analysed for progesterone, estradiol and prostaglandin F2α hormone (PGF2α) concentrations, and fatty acids profile. The results indicated that the group with a very low ratio of n-6:n3 fatty acids (diet 1) showed increased concentration of progesterone in the plasma (P<0.05), while the group with high ratio of n6:n3 fatty acids (diet 3) with high arachidonic acid (AA) showed significantly (P<0.05) increased concentration of estradiol and PGF2α in the plasma. The n-3 polyunsaturated fatty acids (PUFA) in plasma of diet 1 group were significantly (P<0.05) higher than the other treatment diet groups. The total n-6 PUFA was significantly higher (P<0.05) in diet 3 group as compared to diet 1 group. In contrast, the number of implanted embryos was significantly lower (P<0.05) in the diet 3 group at 15 days of gestation, while, the litter size were significantly lower in the diet 2 group and diet 3 group by 4.57 and 1.00 folds respectively, as compared to the control group (diet 4). In conclusion, for the rat with very low ratio on diet 1, the n-6:n-3 ratio satisfied the requirement for the growth of mother and fetuses but was inadequate for the normal process of parturition, probably through inadequate production of the prostaglandins involved.Key words: n-6:n-3, plasma fatty acids, progesterone, estradiol, prostaglandin production, pregnant rat
Effect of Organic Alternative Scouring Agents on Structure of Cellulose/Polyester Blend Fabric
This study investigated the effect of organic alternative scouring
agents on structure of cotton/polyester blend fabric. A structural
modification for a cellulose/polyester blend has been carried out using
(COOH)2, CH3COOH and CH3CH2OH alternative scouring agents and NaOH was
used as control. The scoured fabrics were then subjected to structural
analysis using X-ray diffraction in order to expose the possible
modifications on the pretreated fabrics. The samples showed
possibilities of being highly competitive with the conventional agent.
These inferences were drawn from the difference in the crystallinity
index of scoured samples (10.10-60.03%), the crystallite size of
scoured samples (6-9 nm) in the crystalline region (6-11 nm), in the
amorphous region, inter-planar spacing of the scoured samples
(0.340-0.350 nm) of the crystalline region and (0.360-0.390 nm) of the
amorphous region of sample and number of crystalline planes of the
scoured sample is (3-10) of the cellulose/polyester blend fabric
samples via X-ray diffraction studies
Cardiovascular disease and the role of oral bacteria
In terms of the pathogenesis of cardiovascular disease (CVD) the focus has traditionally been on dyslipidemia. Over the decades our understanding of the pathogenesis of CVD has increased, and infections, including those caused by oral bacteria, are more likely involved in CVD progression than previously thought. While many studies have now shown an association between periodontal disease and CVD, the mechanisms underpinning this relationship remain unclear. This review gives a brief overview of the host-bacterial interactions in periodontal disease and virulence factors of oral bacteria before discussing the proposed mechanisms by which oral bacterial may facilitate the progression of CVD
Time-related and sequential developmental horizons of Sahel goat foetuses
The aim of the present study was to follow the time related developmental changes of caprine foetuses to aid in their age determination. Certain gross external features along with body size changes were studied. Twenty five pregnant Sahel does of known gestational age managed under controlled conditions were used for the study. Absolute changes in the body weight, crown-rump length and height at withers and their relative growth changes compared to body size had similar and significant growth relationships. All the parameters showed high positive significant correlation with the foetal age and with each other with r- values ranging from 0.975 to 0.998. A chronological guide on age estimation of caprine foetuses at 28 day gestational interval, evolved from observations based on developmental horizons. The present study has provided a baseline data which could be applied for age estimation of Sahel goat foetuses. It has also indicated that, it is necessary to employ many criteria involving physiological horizons to estimate the age of a foetus age within narrow limits
Effects of Storage Conditions on Pharmacokinetics of Paracetamol Table
The effects of storage condition on the pharmacokinetics of paracetamol tablets were studied under several storage conditions. Three storage sites which were considered suboptimal were selected. Freshly purchased 1000-tablets tins of paracetamol were bought and stored in each of these sites for not less than two months and were being dispensed. Prior to storage a few tablets from each tin were selected to serve as control. Kinetic studies were carried out with tablets obtained after two months of storage in these sites.
A colorimetric method was used for plasma concentration determination and data analysed using student t test. p value less than 0.05 was considered significant.
Blood level data obtained from the studies were used to determine the pharmacokinetics of the drug. Plasma concentrations showed no statistical differences from two storage sites (p>0.05). The time taken to attain peak plasma level (t) was not statiscally affected in all the sites (p>0.05), but area under the curve from zero to infinity (AuC-) was statistically affected in two sites (
Effect of Intravenous Naftidrofuryl on Transcutaneous Oxygen Pressure in Severe Peripheral Vascular Disease
Experimental Investigation on the Effect of Tool Rotational Speed on Mechanical Properties of AA6082-T6 Friction Stir-Welded Butt Joints
Thigh-length compression stockings and DVT after stroke
Controversy exists as to whether neoadjuvant chemotherapy improves survival in patients with invasive bladder cancer, despite randomised controlled trials of more than 3000 patients. We undertook a systematic review and meta-analysis to assess the effect of such treatment on survival in patients with this disease