The Effect of M. vaccae Preimmunization on Anxiety- and Panic-like Behaviors

The coevolution of microorganisms and their hosts have resulted in the formation of symbiotic relationships between both organisms. These formed associations have in turn benefited larger animals by enhancing their adaption towards external environments and protecting against pathogens. Recent studies have further highlighted the importance of microbial interactions by analyzing their role in proper immune regulation and their effect on behavior and health. Faulty immunoregulatory circuits have been implicated in the rapid rise of chronic inflammatory diseases and considered a risk factor for psychiatric diseases. Although studies have begun to understand the link between microbes and stress-related behaviors, few studies have focused on the potential influence of environmental bacteria on anxiety related disorders. In this study we show that rats that were subcutaneously immunized with heat–killed M. vaccae, a non-pathogenic environmental bacterium, expressed reduced levels of anxiety-related behaviors when tested in the elevated T-maze. As compared to the control group, M. vaccae-treated rats expressed significantly lower latencies when measuring inhibitory avoidance, a behavior related to generalized-anxiety. During the escape task, a behavior associated with panic behavior, M. vaccae treated rats had larger latencies to leave the open arm, but the differences between groups were not significant. The results of our studies suggest a beneficial effect of heat-killed M. vaccae on anxiety-related behaviors in the elevated T-maze. Our study supports the on-going hypothesis regarding the beneficial role of immunoregulatory environmental microbes in control of emotional behavior and emotional states

Diplomado de profundización CISCO.

Luego del estudio de los capítulos de la plataforma de cisco y la realización de las actividades requeridas durante el desarrollo de la asignatura, realizando la ejecución del plan de pruebas para la configuración y comunicación de los diferentes elementos que compongan la topología de cada escenario procedemos a crear el presente documento, que se realiza con el fin de presentar el desarrollo de los ejercicios de la prueba final de habilidades, para dar solución a estos escenarios se requirió aplicar los conceptos y habilidades adquiridos durante el desarrollo de los diferentes capítulos y actividades vistos, mediante los cuales se obtuvo la información sobre los diferentes elementos que conforman una red, protocolos de enrutamiento, parámetros de seguridad y acceso en diferentes dispositivos en la red, además de las configuraciones OSPF, EIGRP, implementación DHCP, NAT, verificación de ACL. Estos escenarios se diseñaron y configuraron según la topología y requerimientos básicos dados, en la aplicación Packet Tracer que permite construir y ejecutar topologías de red. En el escenario 1 se monta y desarrolla un red que requiere de la aplicación de los conocimientos en cuanto a configuración de los hosts necesarios y en una tabla la cual contiene el direccionamiento de cada uno de ellos, así como los servicios ACL, EIGRP, enlaces troncales y la implementación de las configuraciónes. En cuanto al escenario 2, se requerirá la aplicación de conocimientos y competencias en la implementación del enrutamiento VLAN, OSPF, Servicio DHCP y configuración de NAT estáticos y de sobrecarga, adicionalmente administrar los accesos a través de una lista de control de accesoAfter studying the chapters of the cisco platform and carrying out the activities required during the development of the subject, carrying out the test plan for the configuration and communication of the different elements that make up the topology of each scenario we proceed to To create this document, which is carried out in order to present the development of the final skills test exercises, to solve these scenarios, it was required to apply the concepts and skills acquired during the development of the different chapters and activities seen, through which information was obtained on the different elements that make up a network, routing protocols, security parameters and access on different devices in the network, in addition to the OSPF, EIGRP, DHCP implementation, NAT, ACL verification settings. These scenarios were designed and configured according to the topology and basic requirements given, in the Packet Tracer application that allows the construction and execution of network topologies. In scenario 1, a network is developed and developed that requires the application of knowledge in terms of configuration of the necessary hosts and in a table which contains the address of each of them, as well as the ACL, EIGRP services, links Trunks and the implementation of the configurations. As for scenario 2, the application of knowledge and skills in the implementation of VLAN routing, OSPF, DHCP service and static and overload NAT configuration will be required, additionally managing access through an access control lis

High-throughput biointerfaces for direct, label-free, and multiplexed metaplasmonic biosensing

In recent years, metaplasmonic biosensors have emerged as a novel counterpart of well-established plasmonic biosensors based on thin metallic layers. Metaplasmonic biosensors offer high potential for sensor miniaturiza-tion, extreme sensitivity biosensing, and high multiplexing capabilities with detection methods free of coupling optical elements. These capabilities make metaplasmonic biosensors highly attractive for Point-of-Care and handled/portable devices or novel On-Chip devices; as a result, it has increased the number of prototypes and potential applications that emerged during the last years. One of the main challenges to achieving fully operative devices is the achievement of high-throughput biointerfaces for sensitive and selective biodetection in complex media. Despite the superior surface sensitivity achieved by metaplasmonic sensors compared to conventional plasmonic sensors based on metallic thin films, the main limitations to achieving high-throughput and multiplexed biosensing usually are associated with the sensitivity and selectivity of the bioin-terface and, as a consequence, their application to the direct analysis of real complex samples. This graphical review discusses the potential challenges and capabilities of different biofunctionalization strategies, biorecog-nition elements, and antifouling strategies to achieve scalable and high-throughput metaplasmonic biosensing for Point-of-Care devices and bioengineering applications like Organs-On-Chip

A continuous method for arsenic removal from groundwater using hybrid biopolymer-iron-nanoaggregates: improvement through factorial designs

BACKGROUND: Due to a variety of toxicological problems, the presence of As(V) in aquifers is a significant concern. Sorption using chitosan doped with iron nanoaggregates results in a green and cheap methodology for its elimination. RESULTS: The hybrid sorbent was characterized by SEM, EDS, TGA, XRD, and FTIR spectroscopy. Its stability against pH and time was determined by ICP-MS, while conventional analytical techniques verified its Fe content. The sum of an individual As(V) removal capacity by chitosan and iron nanoaggregates was smaller than that of the hybrid sorbent, indicating the existence of synergy. CONCLUSION: This study demonstrates the great capacity of the hybrid sorbent to eliminate As(V) working with a continuous system (columns). The additional use of a factorial design allows for determining of optimal operating values to optimize two responses. In other words, in this multi-response system, column service time (tb) was minimized and, at the same time, it maximized the volumes of purified water obtained ([As(V)] <0.05 m L−1) using desirability function.Fil: Batistelli, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Perez Mora, Barbara Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; ArgentinaFil: Mangiameli, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; ArgentinaFil: Mamana, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura. Laboratorio de Materiales Cerámicos; ArgentinaFil: Lopez, Gerardo. NANOTEK S.A. Parque Tecnológico Litoral Centro; ArgentinaFil: Goddio, María F.. NANOTEK S.A. Parque Tecnológico Litoral Centro; ArgentinaFil: Bellú, Sebastián Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; ArgentinaFil: González, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; Argentin

Characterization of the Flash-LED Lamp: EBNeuro Used to Acquired Visual Evoked Potentials in Rats

El propósito de esta investigación fue caracterizar espacialmente la intensidad luminosa promedio de la lámpara Flash LED (marca EBNeuro-Italia) para evaluar si es apta como fuente de estimulación visual para la adquisición de PEVs en un modelo murino.La señal de la amplitud en análisis de Potenciales Evocados Visuales (PEVs) es una variable que depende del tipo de los electrodos, de la fuente luminosa, del estímulo visual y por consecuente, de la intensidad luminosa por lo que es fundamental reportarla para cada diseño experimental y así, garantizar su reproducibilidad. El objetivo de este trabajo es caracterizar una lámpara con 96 LEDs para la adquisición de PEVs en ratas. Se midió la iluminancia y la intensidad luminosa promedio en un sistema espacial XYZ de 8 cm3 aplicable a un sistema estereotáxico para la fijación de ratas. Se realizaron desplazamientos cada 2 cm en cada plano. Se observó que debido a la distribución geométrica de los LEDs la distribución de la iluminancia no sigue la ley del inverso cuadrado, ya que aumenta conforme la lámpara se aleja. Finalmente, se seleccionó una coordenada para la colocación del ojo de la rata empleando una intensidad luminosa promedio para la adquisición del PEV de 1.043 cd e iluminancia de 128.77 luxes a una distancia ojo-lámpara de 9 cm. Una vez caracterizada la intensidad luminosa y de acuerdo con los PEVs obtenidos, esta lámpara puede utilizarse para estudios PEV en ratas en investigaciones posteriores.UAEMex INNN Se agradece a la SIEA-UAEMex por el apoyo recibido mediante el proyecto 4348/2017/CI. Así como al CONACYT a través del proyecto CB-2011-01-169558 y a la beca de doctorado otorgada a la alumna con número de CVU: 473019

Elliptic and hyperelliptic magnetohydrodynamic equilibria

The present study is a continuation of a previous one on "hyperelliptic" axisymmetric equilibria started in [Tasso and Throumoulopoulos, Phys. Plasmas 5, 2378 (1998)]. Specifically, some equilibria with incompressible flow nonaligned with the magnetic field and restricted by appropriate side conditions like "isothermal" magnetic surfaces, "isodynamicity" or P + B^2/2 constant on magnetic surfaces are found to be reducible to elliptic integrals. The third class recovers recent equilibria found in [Schief, Phys. Plasmas 10, 2677 (2003)]. In contrast to field aligned flows, all solutions found here have nonzero toroidal magnetic field on and elliptic surfaces near the magnetic axis.Comment: 9 page

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Genome Sequence of the Pea Aphid Acyrthosiphon pisum

Aphids are important agricultural pests and also biological models for studies of insect-plant interactions, symbiosis, virus vectoring, and the developmental causes of extreme phenotypic plasticity. Here we present the 464 Mb draft genome assembly of the pea aphid Acyrthosiphon pisum. This first published whole genome sequence of a basal hemimetabolous insect provides an outgroup to the multiple published genomes of holometabolous insects. Pea aphids are host-plant specialists, they can reproduce both sexually and asexually, and they have coevolved with an obligate bacterial symbiont. Here we highlight findings from whole genome analysis that may be related to these unusual biological features. These findings include discovery of extensive gene duplication in more than 2000 gene families as well as loss of evolutionarily conserved genes. Gene family expansions relative to other published genomes include genes involved in chromatin modification, miRNA synthesis, and sugar transport. Gene losses include genes central to the IMD immune pathway, selenoprotein utilization, purine salvage, and the entire urea cycle. The pea aphid genome reveals that only a limited number of genes have been acquired from bacteria; thus the reduced gene count of Buchnera does not reflect gene transfer to the host genome. The inventory of metabolic genes in the pea aphid genome suggests that there is extensive metabolite exchange between the aphid and Buchnera, including sharing of amino acid biosynthesis between the aphid and Buchnera. The pea aphid genome provides a foundation for post-genomic studies of fundamental biological questions and applied agricultural problems

A Roadmap for HEP Software and Computing R&D for the 2020s

Particle physics has an ambitious and broad experimental programme for the coming decades. This programme requires large investments in detector hardware, either to build new facilities and experiments, or to upgrade existing ones. Similarly, it requires commensurate investment in the R&D of software to acquire, manage, process, and analyse the shear amounts of data to be recorded. In planning for the HL-LHC in particular, it is critical that all of the collaborating stakeholders agree on the software goals and priorities, and that the efforts complement each other. In this spirit, this white paper describes the R&D activities required to prepare for this software upgrade.Peer reviewe

Forward-central two-particle correlations in p-Pb collisions at root s(NN)=5.02 TeV

Two-particle angular correlations between trigger particles in the forward pseudorapidity range (2.5 2GeV/c. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B. V.Peer reviewe