Imaging of detached shells around the carbon stars R Scl and U Ant through scattered stellar light

We present the first optical images of scattered light from large, detached gas/dust shells around two carbon stars, R Scl and U Ant, obtained in narrow band filters centred on the resonance lines of neutral K and Na, and in a Str. b filter (only U Ant). They confirm results obtained in CO radio line observations, but also reveal new and interesting structures. Towards R Scl the scattering appears optically thick in both the K and Na filters, and both images outline almost perfectly circular disks with essentially uniform intensity out to a sharp outer radius of 21". These disks are larger -- by about a factor of two -- than the radius of the detached shell which has been marginally resolved in CO radio line data. In U Ant the scattering in the K filter appears to be, at least partially, optically thin, and the image is consistent with scattering in a geometrically thin (3") shell (radius 43") with an overall spherical symmetry. The size of this shell agrees very well with that of the detached shell seen in CO radio line emission. The scattering in the Na filter appears more optically thick, and the image suggests the presence of at least one, possibly two, shells inside the 43" shell. There is no evidence for such a multiple-shell structure in the CO data, but this can be due to considerably lower masses for these inner shells. Weak scattering appears also in a shell which is located outside the 43" shell. The present data do not allow us to conclusively identify the scattering agent, but we argue that most of the emission in the K and Na filter images is to due to resonance line scattering, and that there is also a weaker contribution from dust scattering in the U Ant data. Awaiting new observational data, our interpretation must be regarded as tentative.Comment: 14 pages, 11 figures, to be published in A&

Insights into the chemical composition of Equisetum hyemale by high resolution Raman imaging

Equisetaceae has been of research interest for decades, as it is one of the oldest living plant families, and also due to its high accumulation of silica up to 25% dry wt. Aspects of silica deposition, its association with other biomolecules, as well as the chemical composition of the outer strengthening tissue still remain unclear. These questions were addressed by using high resolution (<1 μm) Confocal Raman microscopy. Two-dimensional spectral maps were acquired on cross sections of Equisetum hyemale and Raman images calculated by integrating over the intensity of characteristic spectral regions. This enabled direct visualization of differences in chemical composition and extraction of average spectra from defined regions for detailed analyses, including principal component analysis (PCA) and basis analysis (partial least square fit based on model spectra). Accumulation of silica was imaged in the knobs and in a thin layer below the cuticula. In the spectrum extracted from the knob region as main contributions, a broad band below 500 cm−1 attributed to amorphous silica, and a band at 976 cm−1 assigned to silanol groups, were found. From this, we concluded that these protrusions were almost pure amorphous, hydrated silica. No silanol group vibration was detected in the silicified epidermal layer below and association with pectin and hemicelluloses indicated. Pectin and hemicelluloses (glucomannan) were found in high levels in the epidermal layer and in a clearly distinguished outer part of the hypodermal sterome fibers. The inner part of the two-layered cells revealed as almost pure cellulose, oriented parallel along the fiber

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Transverse-momentum and pseudorapidity distributions of charged hadrons in pp collisions at √s=7 TeV

This is the pre-print version of the Published Article which can be accessed from the link below.Charged-hadron transverse-momentum and pseudorapidity distributions in proton-proton collisions at √s=7  TeV are measured with the inner tracking system of the CMS detector at the LHC. The charged-hadron yield is obtained by counting the number of reconstructed hits, hit pairs, and fully reconstructed charged-particle tracks. The combination of the three methods gives a charged-particle multiplicity per unit of pseudorapidity dNch/dη||η|<0.5=5.78±0.01(stat)±0.23(syst) for non-single-diffractive events, higher than predicted by commonly used models. The relative increase in charged-particle multiplicity from √s=0.9 to 7 TeV is [66.1±1.0(stat)±4.2(syst)]%. The mean transverse momentum is measured to be 0.545±0.005(stat)±0.015(syst)  GeV/c. The results are compared with similar measurements at lower energies

First measurement of the underlying event activity at the LHC with root s=0.9 TeV

A measurement of the underlying activity in scattering processes with p (T) scale in the GeV region is performed in proton-proton collisions at root s = 0.9 TeV, using data collected by the CMS experiment at the LHC. Charged particle production is studied with reference to the direction of a leading object, either a charged particle or a set of charged particles forming a jet. Predictions of several QCD-inspired models as implemented in PYTHIA are compared, after full detector simulation, to the data. The models generally predict too little production of charged particles with pseudorapidity |eta| LT 2, p (T) GT 0.5 GeV/c, and azimuthal direction transverse to that of the leading object

IPHAS extinction distances to planetary nebulae

Original article can be found at: http://www.aanda.org Copyright European Southern ObservatoryAims. The determination of reliable distances to planetary nebulae (PNe) is a major difficulty in the study of this class of objects in the Galaxy. The availability of new photometric surveys such as IPHAS (the INT/WFC photometric Hα survey of the northern Galactic plane) covering large portions of the sky provide an opportunity to apply the so-called extinction method to determine the distances of a large number of objects. Methods. The technique is applied to a sample of 137 PNe located between –5 and 5 degrees in Galactic latitude, and between 29.52 and 215.49 degrees in longitude. The characteristics of the distance-extinction method and the main sources of errors are carefully discussed. Results. The data on the extinction of the PNe available in the literature, complemented by new observations, allow us to determine extinction distances for 70 PNe. A comparison with statistical distance scales from different authors is presented.Peer reviewe

The peculiar dust shell of Nova DZ Cru (2003)

We present Spitzer Space Telescope observations of the "peculiar variable" DZ Cru, identified by Rushton et al. (2008, MNRAS, 386, 289) as a classical nova. A dust shell, on which are superimposed a number of features, is prominent in the 5-35micron range some 4 years after eruption. We suggest that the dust in DZ Cru is primarily hydrogenated amorphous carbon in which aliphatic bands currently predominate, and which may either become predominantly aromatic as the dust is photo-processed by ultraviolet radiation from the stellar remnant, or more likely completely destroyed.Comment: MNRAS letters, accepte

Ad intrusiveness of location-based advertising - A virtual reconstruction

An important problem for advertisers is the general tendency to avoid advertising (Li et al., 2002; McCoy et al., 2008; Zanot, 1984). Ads typically get the 'blame' that they do not meet the goals and desires of consumers, thus the commercial message is perceived as disturbing and therefore avoided (Li et al., 2002, McCoy et al., 2008; Speck and Elliott, 1997). In order to reduce interference and ad avoidance, advertisers must use forms of marketing which reflect the situation of the consumer (Cho and Cheon, 2004)