11 research outputs found
Urban settlements in intermediate cities, Gujarat State, India : includes case studies and an upgrading project in Rajkot.
Thesis. 1979. M.Arch.A.S.--Massachusetts Institute of Technology. Dept. of Architecture.MICROFICHE COPY AVAILABLE IN ARCHIVES AND ROTCH.Bibliography: p. 80.M.Arch.A.S
One step pre-hardening micropropagation of Bambusa balcooa Roxb.
Bamboo’s fast growth and ability to sequester atmospheric carbon consequently mitigate climate change.Abellon’s mission is to reduce CO2 from the environment by growing bamboo on marginal land and using this biomass for bioenergy. Bambusa balcooa Roxb. micropropagation protocol is established for a consistent supply of quality plantlets. Surface sterilization of nodal explants using 0.1% mercuric chloride followed by initiation in liquid and solid media with and without Gentamycin (3.0-8.0 mg/L) containing Murashige and Skoog (MS) media supplemented with 0.01% myo-inositol, 3% sugar, 25 mg/L citrate 50 mg/L ascorbate, and 3.5 mg/L 6-benzylaminopurine (BAP) as growth regulator. Shoots were multiplied using MS medium augmented with 3% sugar, 6% agar, 0.01% myo-inositol with 3 mg/L BAP and 0.5 mg/L naphthalene acetic acid (NAA). Shoot clusters were rooted in MS supplemented with 4 mg/L NAA, pre-hardened in half MS, acclimatized using coco peat, and vermicompost while at net house using vermicompost, soil, sand, and vesicular arbuscular mycorrhiza (VAM) culture. 97% bud break was achieved from initiation while overall success ratio for establishment was 85%. Sub-culturing shoots showed proliferation rate of 3.5-fold. Rooting was successfully achieved with 83% rate.Acclimatization rate at primary and secondary hardening was 72%. The uniqueness of the study lies as bamboo confines to tropical region, however; we have described B. balcooa mass multiplication protocol using explants from arid region with one step pre-hardening process. The protocol achieved multiplication rate of 3.5-fold, overall survival rate 74.66% using vermicompost and VAM for acclimatization of B. balcooa
Antibacterial activity of methanolic and acetone extract of some medicinal plants used in indian folklore
Antibacterial study of methanolic and acetone extract in crude and treated (with 50 % lead acetate) form of medicinal plants Alstonia scholaris Linn. R.Br. (Stem bark, Apocynaceae), Achyranthus aspera Linn. (Whole plant, acantheceae), Moringa oleifera Lam. (Leaves, Morinaceae), Tinospora cordifolia (Stem, Menispermaceae), and Enicostema hyssopifolium (Willd) (Stem, Gentianaceae). Extractive values in methanol were found higher then the extractive value in acetone, for all plants. All extract of plants were tried at 40-mg/ml concentrations against eight strains of bacteria, by agarwell-difusion test. Acetone extract was found more active as compared to methanol extract. Phytochemical investigation revealed crude and treated extracts of all plants were containing more or less same type of chemical constituents (except protein and carbohydrate). Selected eight strains of bacteria were study for antibiotic susceptibility against standard antibiotics like Ampicillin (10µg), Tetracycline (25µg), Gentamicin (30µg), Co-Trimoxazole (25µg), Amikacin (10µg), by Octadisc. Treated extract of M. oleifera and A. scholaris were count as to new source of antimicrobial agent for the infectious diseases (Typhoid). Keywords: Antibacterial activity, Medicinal Plants, infectious diseases
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE ARTICALTICLE BIO TECHENOLOGY ANTIOXIDANT & ANTIMICROBIAL ACTIVITY OF IN VIVO AND IN VITRO GROWN PLANTS OF Phyllanthus niruri L.
Since ancient time, Phyllunthus niruri L. are using in many herbal preparations. Increasing demand of P. niruri lead to adulteration, and thus there is a global need to develop quality plant material. In present investigation, an efficient in vitro technology was developed for a rapid and large-scale production of P. niruri via axillary bud proliferation using nodal segments of the mature plants. Elongated shoots were cultured on half strength MS medium fortified with various auxins such as NAA, 2, 4-D, IBA, IAA and at different concentrations (0.5- 5.0 mg/l). In vitro developed plantlets were harden and acclimatized in natural environment. The antimicrobial activity (by well diffusion method) and antioxidant activity (by DPPH scavenging assay, reducing power and total antioxidant capacity methods) of in vitro developed plant, naturally grown plant and commercial available sample of P.niruri were determined. Results of antimicrobial activity of methanol extract from in vitro cultured P.niruri showed great potential as source of antimicrobial agent. In DPPH scavenging assay IC50 value of the three extracts was found 49.24µg/ml (in vitro), 41.66µg/ml (in vivo) and 36.14µg/ml (market sample) while the IC50 value of the reference standard ascorbic acid was 53.89 µg/ml. Results suggest in vitro developed plant posses highest antimicrobial and antioxidant activity and thus developed in vitro cultivation technology would provide quality plant material for medicine. This article can be downloaded from www.ijpbs.net B- 78KEY WORDS Phyllunthus niruri, axillary bud proliferation, DPPH scavenging, antioxidant activit