Evaluation of common bean (Phaseolous vulgaris L.) genotypes against anthracnose (Colletotrichum lindemuthianum Sacc. and Magn.)

Saabunud / Received 21.10.2021 ; Aktsepteeritud / Accepted 27.12.2021 ; Avaldatud veebis / Published online 27.12.2021 ; Vastutav autor / Corresponding author: Sujata K.C. [email protected] genotypes of common bean were evaluated against anthracnose under natural epiphytotic conditions. This study was carried out in a randomized complete block design with three replications at the research field of Agriculture Research Station (ARS), Vijaynagar, Jumla, Nepal from June to September 2018. The area under the disease progress curve (AUDPC) and disease severity were calculated. In laboratory conditions, artificial inoculation was carried out on detached leaves of twelve genotypes using a pure culture suspension of Colletotrichum lindemuthianum (1.2× 106 conidia ml–1) in a completely randomized design with three replications. The results showed that bean genotypes varied significantly for disease severity both in the field and laboratory conditions. In the field, bean genotypes showed resistance to highly susceptible reactions. Their AUDPC value ranged from 120.55 to 502.31. The lowest mean AUDPC value was recorded in KBL-1 (120.55) followed by KBL-3 (123.79) and KBL-2 (124.44). Similarly, the lowest severity value was recorded with KBL-1 (0.51), KBL-2 (0.52) and KBL-3 (0.53). Detached leaf assay in laboratory experiment showed that the lowest mean AUDPC was found in KBL-2 (16.67) and KBL-3 (16.67). Therefore, KBL-2 and KBL-3 could be utilized as resistant varieties to anthracnose disease under Jumla and similar field conditions

A review on clubroot of crucifers: symptoms, life-cycle of pathogen, factors affecting severity, and management strategies

Clubroot is a devastating disease of crucifers throughout the world. It is caused by a soil-borne obligate phytoparasite, Plasmodiophora brassicae Wor. Plant affected by this disease shows flagging of leaves, unthrifty growth, and even premature death. When uprooted, root shows characteristic symptom of hypertrophied club-shaped root system. Several biotic and abiotic factors affect the disease severity. Biotic factors include spore load in soil and virulence of pathogen, whereas abiotic factors generally include soil environmental factors such as soil temperature, soil pH, soil moisture, and soil type. Pathogen survives, for substantial period of time in absence of host, through its double-walled resting spores in soil or crop debris. Temperature affects spore germination, occurrence, and pathogen proliferation. Acidic soil reaction is crucial for pathogen to proliferate, metabolize, secret enzymes, and to complete life-cycle. All type of soil textures favor disease; however, severity differs with type of soil and soil organic matter content. Soil moisture provides platform to move bi-flagellated zoospores to infect root hairs of crops. Root hair infection is commensurate with inoculum density or spore load in soil. Immediate management strategies entail cultural practices, use of biocontrol agents, and application of chemical as last resort. Trichoderma spp., Pseudomonas fluorescens, Bacillus subtilis, and Gliocladium catenulatum are potential biocontrol agents. Flusalfamide, Fluazinam, and Cyazofamid are some common chemicals used to control clubroot. Soil carried by farm implements, human body, irrigation water, and flood can be potential source of pathogen. The risk of clubroot can be reduced by ensuring phyto-sanitory measures, destroying host crop debris, regular scouting, growing resistant cultivars, avoiding acidic soil reaction, eliminating weedy hosts, and reducing soil movement

Evaluation of wheat (Triticum aestivum L.) genotypes for spot blotch (Bipolaris sorokiniana Sacc) resistance in terai condition of Nepal

Spot blotch caused by Bipolaris sorokiniana is a major disease of wheat in warm and humid regions of Nepal. The fungus has a worldwide distribution but as a pathogen, it is the most aggressive under the conditions of high relative humidity and temperature associated with the low fertility of soils in Nepal. The yield loss due to the disease is very significant in Nepal. This experiment was conducted to identify the genotypes having a good level of resistance against spot blotch. The experiment set was received from CIMMYT comprises 52 genotypes and arranged in alpha lattice design with two replications in 2017/18 at National Wheat Research Program, Bhairahawa, Nepal, and Regional Agricultural Research Station, Parwanipur, Bara, Nepal. Each plot size was 8 rows of 2 meters long. Three times disease scoring was done in the double-digit method and calculated the Area under the disease progress curve (AUDPC). Other data were analyzed by using R software (4.2.2). Heading days, days to maturity, plant height, number of grains per spike (NGPS), number of tillers per meter square (NTPM), mean AUDPC, thousand-grain weight (TGW), and grain yield were found highly significant. The genotype 8HLBSN47 was found the highest yielder (4996kg/ha) with a 304 mean AUDPC value. Seventeen genotypes (15.3%) found the lowest mean AUDPC, Penultimate leaf AUDPC, Flag leaf AUDPC, and the highest number of tillers per square meter, number of grains per spike, thousand-grain weight, and grain yield

Eco-Friendly Management of Root Knot Nematode (Meloidogyne spp.) in Okra (Abelmoschus Esculentus) Using Different Soil Amendments

Field experiment was conducted from March to July 2022 to identify the eco-friendly management of root-knot nematodes (Meloidogyne spp.) in okra (Abelmoschus esculentus) by using different soil amendments. The experiment was designed as a randomized complete block design with three replications and seven treatments viz., mustard oil cake at 1.41 kg/2m2 plot, poultry manure at 5.6 kg/2m2 plot, goat manure at 3.25 kg/2m2 plot, vermicompost at 4.30 kg/2m2 plot, nitrogen-phosphorus-potassium (NPK) at 125:110:110 g/2m2, Cartap hydrochloride 4% granule (GR) at 10 g/2m2 plot and control. Disease parameters such as root gall index and number of galls per plant, fresh shoot weight, and fresh root weight were recorded at 72, 105, and 120 days after sowing. Fruit yield was recorded after the final pod harvest. Cartap hydrochloride 4G, poultry manure, and mustard oil cake significantly reduced the root gall index as compared to the control. However, goat manure, vermicompost nitrogen, phosphorus, and potassium (NPK) did not give satisfactory results in reducing root gall index. The maximum yield of okra was recorded in the NPK treated plots although the root gall index was not much reduced. The research results indicated that the most effective treatment for reducing root-knot nematode disease and improving vegetative growth and yield of okra was poultry manure. Therefore, poultry manure along with mustard oil cake could be used as an alternative to highly hazardous and persistent chemical nematicides for the management of root-knot nematodes in okra

Thigh-length compression stockings and DVT after stroke

Controversy exists as to whether neoadjuvant chemotherapy improves survival in patients with invasive bladder cancer, despite randomised controlled trials of more than 3000 patients. We undertook a systematic review and meta-analysis to assess the effect of such treatment on survival in patients with this disease

Azithromycin in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial

Background Azithromycin has been proposed as a treatment for COVID-19 on the basis of its immunomodulatory actions. We aimed to evaluate the safety and efficacy of azithromycin in patients admitted to hospital with COVID-19. Methods In this randomised, controlled, open-label, adaptive platform trial (Randomised Evaluation of COVID-19 Therapy [RECOVERY]), several possible treatments were compared with usual care in patients admitted to hospital with COVID-19 in the UK. The trial is underway at 176 hospitals in the UK. Eligible and consenting patients were randomly allocated to either usual standard of care alone or usual standard of care plus azithromycin 500 mg once per day by mouth or intravenously for 10 days or until discharge (or allocation to one of the other RECOVERY treatment groups). Patients were assigned via web-based simple (unstratified) randomisation with allocation concealment and were twice as likely to be randomly assigned to usual care than to any of the active treatment groups. Participants and local study staff were not masked to the allocated treatment, but all others involved in the trial were masked to the outcome data during the trial. The primary outcome was 28-day all-cause mortality, assessed in the intention-to-treat population. The trial is registered with ISRCTN, 50189673, and ClinicalTrials.gov, NCT04381936. Findings Between April 7 and Nov 27, 2020, of 16 442 patients enrolled in the RECOVERY trial, 9433 (57%) were eligible and 7763 were included in the assessment of azithromycin. The mean age of these study participants was 65·3 years (SD 15·7) and approximately a third were women (2944 [38%] of 7763). 2582 patients were randomly allocated to receive azithromycin and 5181 patients were randomly allocated to usual care alone. Overall, 561 (22%) patients allocated to azithromycin and 1162 (22%) patients allocated to usual care died within 28 days (rate ratio 0·97, 95% CI 0·87–1·07; p=0·50). No significant difference was seen in duration of hospital stay (median 10 days [IQR 5 to >28] vs 11 days [5 to >28]) or the proportion of patients discharged from hospital alive within 28 days (rate ratio 1·04, 95% CI 0·98–1·10; p=0·19). Among those not on invasive mechanical ventilation at baseline, no significant difference was seen in the proportion meeting the composite endpoint of invasive mechanical ventilation or death (risk ratio 0·95, 95% CI 0·87–1·03; p=0·24). Interpretation In patients admitted to hospital with COVID-19, azithromycin did not improve survival or other prespecified clinical outcomes. Azithromycin use in patients admitted to hospital with COVID-19 should be restricted to patients in whom there is a clear antimicrobial indication. Funding UK Research and Innovation (Medical Research Council) and National Institute of Health Research