Bone Marrow Stem Cell Treatment for Ischemic Heart Disease in Patients with No Option of Revascularization: A Systematic Review and Meta-Analysis

PMCID: PMC3686792This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Enhanced Antimicrobial activities of Hybrid ZnMgAlO nanocomposite by soft chemical method

In the present investigation, ZnMgAlO nanoparticles were prepared by soft chemical method.  The synthesized NPs were analyzed by XRD and SEM EDAX. ZnMgAlO crystal structure was confirmed through powder XRD technique as hexagonal wurtzite structure. The surface morphology was analyzed from SEM images. Finally, antimicrobial activity of all the synthesized samples was tested against Bacillus subtilis and Chlamydia trachomatis bacteria and Xylaria hypoxylon, Fistulina hepatica fungus. The observed results showed good anti-bacterial and anti-fungal activities. Â

Brain Neoplasm Classification & Detection of Accuracy on MRI Images

The abnormal, uncontrolled cell growth in the brain, commonly known n as a brain tumor, can lead to immense pressure on the various nerves and blood vessels, causing irreversible harm to the body. Early detection of brain tumors is the key to avoiding such compilations. Tumour detection can be done through various advanced Machine Learning and Image Processing algorithms. Mind Brain tumors have demonstrated testing to treat, to a great extent inferable from the organic qualities of these diseases, which frequently plan to restrict progress. To begin with, by invading one of the body's most significant organs, these growths are much of the time situated past the compass of even the most gifted neurosurgeon. These cancers are likewise situated behind the blood-cerebrum boundary (BBB), a tight intersection and transport proteins that shield fragile brain tissues from openness to factors in the overall flow, subsequently obstructing openness to foundational chemotherapy [6,7]. Besides, the interesting formative, hereditary, epigenetic and micro environmental elements of the cerebrum much of the time render these tumors impervious to ordinary and novel medicines. These difficulties are accumulated by the uncommonness of cerebrum growths comparative with numerous different types of disease, restricting the degree of subsidizing and interest from the drug business and drawing in a moderately little and divided research local area

PRODUCTION & CHARACTERIZATION OF BIOPLASTICS FROM VEGETABLE PEELS-AN IDEAL STRATEGY FOR FOOD WASTE DISPOSAL

The use of bioplastics has gained momentum as it is biodegradable. Use of cost effective and easily available substrates in the production of polyhydroxy butyrate (PHB) enhances its application and hence PHB can be successfully used as a substitute for plastics. The present work was performed to study the optimum production of polyhydroxy butyrate (PHB) by bacteria isolated from soil and effluent samples using vegetable peels as carbon source and its efficiency was checked by PHB production at different time intervals. The maximum PHB production was observed with potato peel as carbon source. Thus, waste food as peels could be utilized as alternate sources of substrates for PHB production. Further investigations are undertaken

Functional proteomics in groundnut: Unveiling resistance mechanisms against stem rot using seaweed and bioinoculant

Sclerotium rolfsii, a soil-borne fungal pathogen with a broad host range, poses a major threat to groundnut cultivation by causing stem rot disease, leading to significant yield losses. In this study, S. rolfsii and five Trichoderma spp. isolates were isolated. PCR amplification using universal fungal primers viz., ITS-1 and ITS-4 confirmed the identity of S. rolfsii via BLAST analysis, which was further validated using species-specific primers SR1-F and SR1-R. The sequence was submitted to GenBank under the accession number MZ920141. Antagonistic potential of five Trichoderma isolates was assessed, among which Trichoderma asperellum (Tr1) exhibited the highest mycelial inhibition (73.81 %) in dual culture and up to 89.11 % inhibition in poisoned food assays. Volatile metabolites from Tr1 significantly suppressed mycelial growth (67.56 %) and sclerotial production (91.22 %). Molecular identification of Tr1 via ITS and TEF1 gene sequencing confirmed it as T. asperellum, with GenBank accession number OL872253. Additionally, solvent extracts of marine macroalgae, particularly Sargassum wightii (10 %), showed potent antifungal activity (87.56 % inhibition). A pot culture study combining Tr1 and S. wightii extract significantly reduced stem rot incidence (84.93 %) and improved plant growth as well as yield parameters. Protein profiling using 2D-PAGE and MALDI-TOF analysis revealed unique expression of defense-related proteins such as Peptidyl-prolyl cis-trans isomerase, bHLH145 and 1, 8-cineole synthase in treated plants. Functional analysis indicated their involvement in auxin transport, transcriptional regulation and secondary metabolite biosynthesis, contributing to plant immune responses. These findings highlight the synergistic potential of Tr1 and marine macroalgal extracts in sustainable management of S. rolfsii, while proteomic insights provide a molecular basis for induced resistance in groundnut

Migration towards SDF-1 selects angiogenin-expressing bone marrow monocytes endowed with cardiac reparative activity in patients with previous myocardial infarction

INTRODUCTION: Chemokine-directed migration is crucial for homing of regenerative cells to the infarcted heart and correlates with outcomes of cell therapy trials. Hence, transplantation of chemokine-responsive bone marrow cells may be ideal for treatment of myocardial ischemia. To verify the therapeutic activity of bone marrow mononuclear cells (BM-MNCs) selected by in vitro migration towards the chemokine stromal cell-derived factor-1 (SDF-1) in a mouse model of myocardial infarction (MI), we used BM-MNCs from patients with previous large MI recruited in the TransACT-1&2 cell therapy trials. METHODS: Unfractioned BM-MNCs, SDF-1-responsive, and SDF-1-nonresponsive BM-MNCs isolated by patients recruited in the TransACT-1&2 cell therapy trials were tested in Matrigel assay to evaluate angiogenic potential. Secretome and antigenic profile were characterized by flow cytometry. Angiogenin expression was measured by RT-PCR. Cells groups were also intramyocardially injected in an in vivo model of MI (8-week-old immune deficient CD1-FOXN1(nu/nu) mice). Echocardiography and hemodynamic measurements were performed before and at 14 days post-MI. Arterioles and capillaries density, infiltration of inflammatory cells, interstitial fibrosis, and cardiomyocyte proliferation and apoptosis were assessed by immunohistochemistry. RESULTS: In vitro migration enriched for monocytes, while CD34(+) and CD133(+) cells and T lymphocytes remained mainly confined in the non-migrated fraction. Unfractioned total BM-MNCs promoted angiogenesis on Matrigel more efficiently than migrated or non-migrated cells. In mice with induced MI, intramyocardial injection of unfractionated or migrated BM-MNCs was more effective in preserving cardiac contractility and pressure indexes than vehicle or non-migrated BM-MNCs. Moreover, unfractioned BM-MNCs enhanced neovascularization, whereas the migrated fraction was unique in reducing the infarct size and interstitial fibrosis. In vitro studies on isolated cardiomyocytes suggest participation of angiogenin, a secreted ribonuclease that inhibits protein translation under stress conditions, in promotion of cardiomyocyte survival by migrated BM-MNCs. CONCLUSIONS: Transplantation of bone marrow cells helps post-MI healing through distinct actions on vascular cells and cardiomyocytes. In addition, the SDF-1-responsive fraction is enriched with angiogenin-expressing monocytes, which may improve cardiac recovery through activation of cardiomyocyte response to stress. Identification of factors linking migratory and therapeutic outcomes could help refine regenerative approaches. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-015-0028-y) contains supplementary material, which is available to authorized users

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A review of economic evaluation models for cardiac resynchronization therapy with implantable cardioverter defibrillators in patients with heart failure

Objectives Cardiac resynchronization therapy with a biventricular pacemaker (CRT-P) is an effective treatment for dyssynchronous heart failure (DHF). Adding an implantable cardioverter defibrillator (CRT-D) may further reduce the risk of sudden cardiac death (SCD). However, if the majority of patients do not require shock therapy, the cost-effectiveness ratio of CRT-D compared to CRT-P may be high. The objective of this study was to systematically review decision models evaluating the cost-effectiveness of CRT-D for patients with DHF, compare the structure and inputs of these models and identify the main factors influencing the ICERs for CRT-D. Methods A comprehensive search strategy of Medline (Ovid), Embase (Ovid) and EconLit identified eight cost-effectiveness models evaluating CRT-D against optimal pharmacological therapy (OPT) and/or CRT-P. Results The selected economic studies differed in terms of model structure, treatment path, time horizons, and sources of efficacy data. CRT-D was found cost-effective when compared to OPT but its cost-effectiveness became questionable when compared to CRT-P. Conclusions Cost-effectiveness of CRT-D may increase depending on improvement of all-cause mortality rates and HF mortality rates in patients who receive CRT-D, costs of the device, and battery life. In particular, future studies need to investigate longer-term mortality rates and identify CRT-P patients that will gain the most, in terms of life expectancy, from being treated with a CRT-D.This work was supported by the Center for Translational Molecular Medicine and The Netherlands Heart Foundation under the ‘Biomarkers to predict cardiac failure, arrhythmias and success of treatment’ (COHFAR) projec