A Centennial Record of Paleosalinity Change in the Tidal Reaches of the Potomac and Rappahannock Rivers, Tributaries to Chesapeake Bay

Gravity and push cores from the Potomac and Rappahannock Rivers (Virginia Tidewater) were collected from central and proximal estuarine zones with known seasonal salinity stratification. The lowermost microfossil associations in the cores comprise alternating ostracode populations of Cyprideis salebrosa and Cytheromorpha. This microfossil association gives way to an oligohaline association dominated by the freshwater ostracode Darwinula stevensoni. Stable oxygen isotope values (δ18O) of Rapphannock Cyprideis salebrosa are highly variable ranging between -6.6 to -3.2‰ VPDB. δ18O values for Potomac Cytheromorpha fuscata range from -8.2 to -3.2‰ VPDB. Positive excursions in δ18O values are synchronous with population peaks for both Cyprideis and Cytheromorpha indicative of increased marine influence and/or higher salinities. Microfossil paleoecology coupled with oxygen isotope values record a marked shift towards gradual freshening and deterioration of the salinity structure in the tidal tributaries during the mid-to late 19th century. We attribute these trends to both decadal climate trends and aggressive land use practices in the Chesapeake Bay watershed during the late 19th to middle 20th centuries

The Grizzly, October 3, 2013

Strategic Plan Update • Ursinus President Fong Discusses Liberal Arts Education in China • Ursinus Students Visit New York City • Upsilon Phi Delta Pledging Plans • Astronomer Speaks • U-Innovate Winners Announced • Psych Professor Joins Campus • Ursinus Mass Email Policy Explained • Opinion: Don\u27t Get Sold on Multi-Level Marketing; Yes, Going to College is Definitely Worth it • UC Athletes Handle a Heavy Workload • Dalrymple Completes Impressive UC Career • Football and Field Hockey Keep Rollinghttps://digitalcommons.ursinus.edu/grizzlynews/1888/thumbnail.jp

The Grizzly, November 14, 2013

Bearitones and B\u27Naturals Perform This Weekend • UCEA Waste Watching at the Philadelphia Marathon • Climate and Sustainability Action Plan Announced • Process of Making New Classes at UC • UCDC Hosts Local Choreographers • Ursinus Athletics Honors Graduates • Wind Ensemble Performance • Opinion: Assign Credit for Varsity Athletics; Academic Probation Policy Exempts Athletics • Women\u27s Basketball Will Rely on Young Talent • UC Wrestlers Ready to Meet Expectations • Exciting Weekend for UC Athleticshttps://digitalcommons.ursinus.edu/grizzlynews/1893/thumbnail.jp

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Phospholipase C-ε Regulates Epidermal Morphogenesis in Caenorhabditis elegans

Migration of cells within epithelial sheets is an important feature of embryogenesis and other biological processes. Previous work has demonstrated a role for inositol 1,4,5-trisphosphate (IP3)-mediated calcium signalling in the rearrangement of epidermal cells (also known as hypodermal cells) during embryonic morphogenesis in Caenorhabditis elegans. However the mechanism by which IP3 production is stimulated is unknown. IP3 is produced by the action of phospholipase C (PLC). We therefore surveyed the PLC family of C. elegans using RNAi and mutant strains, and found that depletion of PLC-1/PLC-ε produced substantial embryonic lethality. We used the epithelial cell marker ajm-1::gfp to follow the behaviour of epidermal cells and found that 96% of the arrested embryos have morphogenetic defects. These defects include defective ventral enclosure and aberrant dorsal intercalation. Using time-lapse confocal microscopy we show that the migration of the ventral epidermal cells, especially of the leading cells, is slower and often fails in plc-1(tm753) embryos. As a consequence plc-1 loss of function results in ruptured embryos with a Gex phenotype (gut on exterior) and lumpy larvae. Thus PLC-1 is involved in the regulation of morphogenesis. Genetic studies using gain- and loss-of-function alleles of itr-1, the gene encoding the IP3 receptor in C. elegans, demonstrate that PLC-1 acts through ITR-1. Using RNAi and double mutants to deplete the other PLCs in a plc-1 background, we show that PLC-3/PLC-γ and EGL-8/PLC-β can compensate for reduced PLC-1 activity. Our work places PLC-ε into a pathway controlling epidermal cell migration, thus establishing a novel role for PLC-ε

The genomic history of the Iberian Peninsula over the past 8000 years

We assembled genome-wide data from 271 ancient Iberians, of whom 176 are from the largely unsampled period after 2000 BCE, thereby providing a high-resolution time transect of the Iberian Peninsula.We document high genetic substructure between northwestern and southeastern hunter-gatherers before the spread of farming.We reveal sporadic contacts between Iberia and North Africa by ~2500 BCE and, by ~2000 BCE, the replacement of 40% of Iberia's ancestry and nearly 100% of its Y-chromosomes by people with Steppe ancestry.We show that, in the Iron Age, Steppe ancestry had spread not only into Indo-European-speaking regions but also into non-Indo-European-speaking ones, and we reveal that present-day Basques are best described as a typical Iron Age population without the admixture events that later affected the rest of Iberia. Additionally, we document how, beginning at least in the Roman period, the ancestry of the peninsula was transformed by gene flow from North Africa and the eastern Mediterranean

Microbial Metabolite Signaling Is Required for Systemic Iron Homeostasis

45 Pág.Iron is a central micronutrient needed by all living organisms. Competition for iron in the intestinal tract is essential for the maintenance of indigenous microbial populations and for host health. How symbiotic relationships between hosts and native microbes persist during times of iron limitation is unclear. Here, we demonstrate that indigenous bacteria possess an iron-dependent mechanism that inhibits host iron transport and storage. Using a high-throughput screen of microbial metabolites, we found that gut microbiota produce metabolites that suppress hypoxia-inducible factor 2α (HIF-2α) a master transcription factor of intestinal iron absorption and increase the iron-storage protein ferritin, resulting in decreased intestinal iron absorption by the host. We identified 1,3-diaminopropane (DAP) and reuterin as inhibitors of HIF-2α via inhibition of heterodimerization. DAP and reuterin effectively ameliorated systemic iron overload. This work provides evidence of intestine-microbiota metabolic crosstalk that is essential for systemic iron homeostasis.This work was supported by the NIH, United States (grants CA148828 and DK095201 to Y.M.S. and F31 DK11655 to A.J.S.); the University of Michigan Center for Gastrointestinal Research, United States (DK034933); a pilot grant from the University of Michigan, United States-GI Spore (CA130810 to Y.M.S.); the University of Michigan, United States Microbiome Explorer Program, NIAID, United States Novel Alternative Model Systems for Enteric Diseases (NAMSED) consortium (U19AI116482 to M.K.S., J.R.S. and V.B.Y.); NIAID, United States (U01AI124255 to M.K.S. and V.B.Y.) Crohn’s & Colitis Foundation, United States Senior Research Award (410234) to N.I.; Clinical and Translational Science Award (CTSA) from The Michigan Institute for Clinical & Health Research (MICHR), United States (UL1TR000433) to D.R.H.; The Pennsylvania Department of Health, United States using Tobacco CURE grant to A.D.P.; and the Spanish Ministry of Science Innovation and Universities, Spain (RTA2017-00002-00-00 to J.L.A.).Peer reviewe

Preferential Amplification of CD8 Effector-T Cells after Transcutaneous Application of an Inactivated Influenza Vaccine: A Randomized Phase I Trial

Background: Current conventional vaccination approaches do not induce potent CD8 T-cell responses for fighting mostly variable viral diseases such as influenza, avian influenza viruses or HIV. Following our recent study on vaccine penetration by targeting of vaccine to human hair follicular ducts surrounded by Langerhans cells, we tested in the first randomized Phase-Ia trial based on hair follicle penetration (namely transcutaneous route) the induction of virus-specific CD8 T cell responses. Methods and Findings: We chose the inactivated influenza vaccine – a conventional licensed tetanus/influenza (TETAGRIP®) vaccine – to compare the safety and immunogenicity of transcutaneous (TC) versus IM immunization in two randomized controlled, multi-center Phase I trials including 24 healthy-volunteers and 12 HIV-infected patients. Vaccination was performed by application of inactivated influenza vaccine according to a standard protocol allowing the opening of the hair duct for the TC route or needle-injection for the IM route. We demonstrated that the safety of the two routes was similar. We showed the superiority of TC application, but not the IM route, to induce a significant increase in influenza-specific CD8 cytokine-producing cells in healthy-volunteers and in HIV-infected patients. However, these routes did not differ significantly for the induction of influenza-specific CD4 responses, and neutralizing antibodies were induced only by the IM route. The CD8 cell response is thus the major immune response observed after TC vaccination. Conclusions: This Phase Ia clinical trial (Manon05) testing an anti-influenza vaccine demonstrated that vaccines designed for antibody induction by the IM route, generate vaccine-specific CD8 T cells when administered transcutaneously. These results underline the necessity of adapting vaccination strategies to control complex infectious diseases when CD8 cellular responses are crucial. Our work opens up a key area for the development of preventive and therapeutic vaccines for diseases in which CD8 cells play a crucial role

The Beaker phenomenon and the genomic transformation of northwest Europe

From around 2750 to 2500 bc, Bell Beaker pottery became widespread across western and central Europe, before it disappeared between 2200 and 1800 bc. The forces that propelled its expansion are a matter of long-standing debate, and there is support for both cultural diffusion and migration having a role in this process. Here we present genome-wide data from 400 Neolithic, Copper Age and Bronze Age Europeans, including 226 individuals associated with Beaker-complex artefacts. We detected limited genetic affinity between Beaker-complex-associated individuals from Iberia and central Europe, and thus exclude migration as an important mechanism of spread between these two regions. However, migration had a key role in the further dissemination of the Beaker complex. We document this phenomenon most clearly in Britain, where the spread of the Beaker complex introduced high levels of steppe-related ancestry and was associated with the replacement of approximately 90% of Britain’s gene pool within a few hundred years, continuing the east-to-west expansion that had brought steppe-related ancestry into central and northern Europe over the previous centuries

Post-Franco Theatre

In the multiple realms and layers that comprise the contemporary Spanish theatrical landscape, “crisis” would seem to be the word that most often lingers in the air, as though it were a common mantra, ready to roll off the tongue of so many theatre professionals with such enormous ease, and even enthusiasm, that one is prompted to wonder whether it might indeed be a miracle that the contemporary technological revolution – coupled with perpetual quandaries concerning public and private funding for the arts – had not by now brought an end to the evolution of the oldest of live arts, or, at the very least, an end to drama as we know it