The Characterization of the Microbiome for the Northern Two-lined Salamander (Eurycea Bislineata) and Its Ecological Interactions with the Pathogen Batrachochytrium Dendrobatidis

Undergraduate Basi

Well Performance Tracking in a Mature Waterflood Asset

Imperial Users onl

Vaccinia protein C16 blocks innate immune sensing of DNA by binding the Ku complex

VACV gene C16L encodes a 37-kDa protein that is highly conserved in orthopoxviruses and functions as an immunomodulator. Intranasal infection of mice with a virus lacking C16L (vΔC16) induced less weight loss, fewer signs of illness and increased infiltration of leukocytes to the lungs compared with wild-type virus. To understand C16’s mechanism of action, tandem affinity purification and mass spectrometry were used to identify C16 binding partners. This revealed that Ku70, Ku80 and PHD2 interact with C16 in cells. Ku70 and Ku80 constitute the Ku heterodimer, a well characterised DNA repair complex. MEFs lacking Ku, or the other component of the DNA-dependent protein kinase (DNA-PK) complex, the catalytic subunit of DNA-PK (DNA-PKcs), were shown to be deficient in the upregulation of IRF-3-dependent genes such as Cxcl10, Il6 and Ifnb in response to transfection of DNA, but not poly (I:C). Furthermore, following infection of MEFs with VACV strain MVA the activation of Cxcl10 or Il6 transcription was dependent on DNA-PK. Therefore, DNA-PK is a DNA sensor capable of detecting poxvirus DNA and activating IRF-3-dependent innate immunity. C16 inhibited the binding of Ku to DNA, and therefore inhibited DNA-mediated induction of Cxcl10 and Il-6 in MEFs. The role of C16 in vivo was also examined: infection with vΔC16 led to increased production of Cxcl10 and Il-6 following intranasal infection of mice compared with wild-type virus. C16 is therefore an inhibitor of DNA-PK-mediated DNA sensing and innate immune activation. C16 was also shown to bind to PHD2, an enzyme involved in regulation of hypoxic signalling. VACV was found to activate the transcription of hypoxia-related genes, and C16 expression in cells was also capable of doing this. The role of hypoxic signalling in VACV infection remains poorly understood

A Quantum Algorithm To Locate Unknown Hashes For Known N-Grams Within A Large Malware Corpus

Quantum computing has evolved quickly in recent years and is showing significant benefits in a variety of fields. Malware analysis is one of those fields that could also take advantage of quantum computing. The combination of software used to locate the most frequent hashes and $n$-grams between benign and malicious software (KiloGram) and a quantum search algorithm could be beneficial, by loading the table of hashes and $n$-grams into a quantum computer, and thereby speeding up the process of mapping $n$-grams to their hashes. The first phase will be to use KiloGram to find the top-$k$ hashes and $n$-grams for a large malware corpus. From here, the resulting hash table is then loaded into a quantum machine. A quantum search algorithm is then used search among every permutation of the entangled key and value pairs to find the desired hash value. This prevents one from having to re-compute hashes for a set of $n$-grams, which can take on average $O(MN)$ time, whereas the quantum algorithm could take $O(\sqrt{N})$ in the number of table lookups to find the desired hash values.Comment: IEEE Quantum Week 2020 Conferenc

A fresh look at paralytics in the critically ill: real promise and real concern.

Neuromuscular blocking agents (NMBAs), or "paralytics," often are deployed in the sickest patients in the intensive care unit (ICU) when usual care fails. Despite the publication of guidelines on the use of NMBAs in the ICU in 2002, clinicians have needed more direction to determine which patients would benefit from NMBAs and which patients would be harmed. Recently, new evidence has shown that paralytics hold more promise when used in carefully selected lung injury patients for brief periods of time. When used in early acute respiratory distress syndrome (ARDS), NMBAs assist to establish a lung protective strategy, which leads to improved oxygenation, decreased pulmonary and systemic inflammation, and potentially improved mortality. It also is increasingly recognized that NMBAs can cause harm, particularly critical illness polyneuromyopathy (CIPM), when used for prolonged periods or in septic shock. In this review, we address several practical considerations for clinicians who use NMBAs in their practice. Ultimately, we conclude that NMBAs should be considered a lung protective adjuvant in early ARDS and that clinicians should consider using an alternative NMBA to the aminosteroids in septic shock with less severe lung injury pending further studies

Data preservation, the new science and the practitioner librarian

This paper outlines the information management principles of the so-called 'new science', and attempts to put these in the context of traditional library and information science principles. It gives a brief review of some work in the area, in particular focussing on the work show-cased by the annual digital preservation conference series hosted by the Digital Curation Centre in Scotland (http://www.dcc.ac.uk/). There is a danger that scientists (as opposed to LIS professionals) will apply the information management techniques of the new science to their own activities inappropriately, especially to research that is best curated as 'old' not new science. This is something on which information professionals are well placed to give advice and make judgements. More practice-oriented research is needed to enhance understanding of how traditional librarianship practices can be applied to the data intensive scientific research carried out by so-called 'virtual organisations'. This paper makes some initial suggestions about how the tools of library and information practice can be related to the 'new science'. In particular, it highlights their relevance to distinguishing between the information management needs of the 'old' and the 'new' sciences: these needs are quite distinct, though easily confused. This paper relates terms from pure science such as the virtual organisation, cyberinfrastructure and e-science to traditional LIS concepts, and tries to create an understanding of the relationship between the two disciplines for the library practitioner

A study of the pion effective mass at finite temperature using the linear sigma model

We have used the Cornwall--Jackiw--Tomboulis method of composite operators and its formulation at finite temperature by Amelino-Camelia and Pi, in order to calculate the effective potential of the O(4) linear sigma model beyond the Hartree approximation. We have obtained a system of gap equations for the effective mass of sigma and the pions as well as for the condensate, the order parameter of the chiral phase transition. We find that the thermal effective mass of the pions at low temperatures remains lower than in the Hartree case, nevertheless deviates from the chiral limit. Our observation is consistent with other results which have been published previously.Comment: Typos in address and references corrected. Latex2e, 5 pages, 2 .eps figures. Contribution to the proceedings of the conference: X Hadron Physics and VII Relativistic Aspects of Nuclear Physics: A joint meeting on QCD and QGP, (HADRON-RANP 2004), 28 March--3 April 2004, Angra dos Reis, Rio de Janeiro, Brazi