Determination of Five Synthetic Colorants in Grain Food by High Performance Liquid Chromatography-tandem Mass Spectrum

A new application of baking soda was developed, the synthetic colorant in cereal was extracted by adding with methanol solution in this paper.To develop new uses of edible bicarbonate,synthetic colorants were extracted from cereals by adding methanol water solution of edible bicarbonate in this paper, Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)was used to test standard recovery.The extraction effect of edible bicarbonate of soda on five synthetic colorants of sunset yellow, amaranth red, carmine red, alluvium red and bright blue in cereals was investigated.The sample was extracted with 70 mL 40% methanol water vortexed with 1g edible baking soda, purified with Strata X-AW SPE column and determined.The results showed that, a good linearity was observed between 0.1 and 1.4μg/mL, and for the 5 synthetic colorants, the limit of detection was between 0.07 and 0.50 μg/g.The recoveries of the 5 synthetic colorants of different food substrates at different spiked levels (1, 2 and 10 μg/g) were all found to be in the recovery range specified in GB/T27404.The recoveries for the 5 synthetic colorants were found between 80.0% and 110% with RSD from 0.7% to 5.1% (n=6).Hence, baking soda added to 40% methanol water solution can be used to extract synthetic colorants from grain products with simple operation, accurate results, high efficiency and environmental protection

Dynamic analysis of optimality in myocardial energy metabolism under normal and ischemic conditions

To better understand the dynamic regulation of optimality in metabolic networks under perturbed conditions, we reconstruct the energetic-metabolic network in mammalian myocardia using dynamic flux balance analysis (DFBA). Additionally, we modified the optimal objective from the maximization of ATP production to the minimal fluctuation of the profile of metabolite concentration under ischemic conditions, extending the hypothesis of original minimization of metabolic adjustment to create a composite modeling approach called M-DFBA. The simulation results are more consistent with experimental data than are those of the DFBA model, particularly the retentive predominant contribution of fatty acid to oxidative ATP synthesis, the exact mechanism of which has not been elucidated and seems to be unpredictable by the DFBA model. These results suggest that the systemic states of metabolic networks do not always remain optimal, but may become suboptimal when a transient perturbation occurs. This finding supports the relevance of our hypothesis and could contribute to the further exploration of the underlying mechanism of dynamic regulation in metabolic networks

2012 ACCF/AHA/ACP/AATS/PCNA/SCAI/STS guideline for the diagnosis and management of patients with stable ischemic heart disease

The recommendations listed in this document are, whenever possible, evidence based. An extensive evidence review was conducted as the document was compiled through December 2008. Repeated literature searches were performed by the guideline development staff and writing committee members as new issues were considered. New clinical trials published in peer-reviewed journals and articles through December 2011 were also reviewed and incorporated when relevant. Furthermore, because of the extended development time period for this guideline, peer review comments indicated that the sections focused on imaging technologies required additional updating, which occurred during 2011. Therefore, the evidence review for the imaging sections includes published literature through December 2011

J. Cent. South Univ. Technol.

A capillary rheometer operating at prescribed temperature and pressure was set up. The measurement principles of the capillary rheometer were introduced. The equipment constant was obtained using standard liquids (e.g. water with known viscosity), and the rheometer was thus calibrated. The different carrier liquids viscosities of Fe3O4 magnetic fluid (MF) were measured at different flow rates. The experimental data were compared with the results of a theoretical equation derived in this study. Finally, the effect of temperature was investigated. Due to the effect of different carrier liquids, xylene-based and silicon oil-based MF show Newton behavior, but the hydroxyethyl-cellulose (HXC)-based MF shows non-Newton behavior of shear thinning. The difference between the experimentally measured viscosity and the theoretical prediction is lower for dilute MF, whereas, the difference is large for highly concentrated MF. The viscosity decreases quickly with the increase of temperature.A capillary rheometer operating at prescribed temperature and pressure was set up. The measurement principles of the capillary rheometer were introduced. The equipment constant was obtained using standard liquids (e.g. water with known viscosity), and the rheometer was thus calibrated. The different carrier liquids viscosities of Fe3O4 magnetic fluid (MF) were measured at different flow rates. The experimental data were compared with the results of a theoretical equation derived in this study. Finally, the effect of temperature was investigated. Due to the effect of different carrier liquids, xylene-based and silicon oil-based MF show Newton behavior, but the hydroxyethyl-cellulose (HXC)-based MF shows non-Newton behavior of shear thinning. The difference between the experimentally measured viscosity and the theoretical prediction is lower for dilute MF, whereas, the difference is large for highly concentrated MF. The viscosity decreases quickly with the increase of temperature