Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

Background: Bacterial lipopolysaccharide and interferon-γ stimulation of rodent macrophages in vitro induces up-regulation of inducible nitric oxide synthase, whereas interleukin-4 stimulation results in increased activity of arginase-1. Thus different stimulants result in differing macrophage phenotypes, appropriate for responses to a range of pathogens. The current study was conducted in order to determine whether bovine macrophages derived from monocytes and spleen respond similarly. Results: Lipopolysaccharide and interferon-γ did not induce detectable increases in nitric oxide production by bovine monocyte-derived or splenic macrophages in vitro. Similarly, interleukin-4 and interleukin-13 did not affect arginase activity. However, changes in transcription of genes coding for these products were detected. Conclusion: Differences between macrophage activation patterns exist between cattle and other species and these differences may occur during the post-transcription phase

Development of an Atmospheric Pressure Ion Mobility Spectrometer–Mass Spectrometer with an Orthogonal Acceleration Electrostatic Sector TOF Mass Analyzer

Recently developed ion mobility mass spectrometer is described. The instrument is based on a drift tube ion mobility spectrometer and an orthogonal acceleration electrostatic sector time-of-flight mass analyzer. Data collection is performed using a specially developed fast ADC-based recorder that allows real-time data integration in an interval between 3 and 100 s. Primary tests were done with positive ion electrospray. The tests have shown obtaining 100 ion mobility resolving power and 2000 mass resolving power. Obtained for 2,6-di-<i>tert</i>-butylpyridine in electrosprayed liquid samples during 100 s analysis and full IMS/MS data collection mode were 4 nM relative limits of detection and a 1 pg absolute limit of detection (S/N=3). Characteristic ion mobility/mass distributions were recorded for selected antibiotics, including amoxicillin, ampicillin, lomefloxacin, and ofloxacin. At studied conditions, lomefloxacin forms only a protonated molecule-producing reduced ion mobility peak at 1.082 cm²/(V s). Both amoxicillin and ampicillin produce [M + H]⁺, [M + CH₃OH + H]⁺, and [M + CH₃CN + H]⁺. Amoxicillin shows two peaks at 0.909 cm²/(V s) and 0.905 cm²/(V s). Ampicillin shows one peak at 0.945 cm²/(V s). Intensity of protonated methanol containing cluster for both ampicillin and amoxicillin has a clear tendency to rise with sample keeping time. Ofloxacin produces two peaks in the ion mobility distribution. A lower ion mobility peak at 1.051 cm²/(V s) is shown to be formed by [M + H]⁺ ions. A higher ion mobility peak appearing for samples kept more than 48 h is shown to be formed by both [M + H]⁺ ion and a component identified as the [M + 2H + M]⁺² cluster. The cluster probably partly dissociates in the interface producing the [M + H]⁺ ion

The pulsations of PG 1351+489

PG 1351+489 is one of the 20DBVs – pulsating helium-atmospherewhite dwarf stars – known and has the simplest power spectrum for this class of star, making it a good candidate to study cooling rates. We report accurate period determinations for the main peak at 489.334 48 s and two other normal modes using data from the Whole Earth Telescope (WET) observations of 1995 and 2009. In 2009, we detected a new pulsation mode and the main pulsation mode exhibited substantial change in its amplitude compared to all previous observations. We were able to estimate the star’s rotation period, of 8.9 h, and discuss a possible determination of the rate of period change of (2.0 ± 0.9) × 10−13 s s-ˡ, the first such estimate for a DBV

The pulsations of PG 1351+489

PG 1351+489 is one of the 20DBVs – pulsating helium-atmospherewhite dwarf stars – known and has the simplest power spectrum for this class of star, making it a good candidate to study cooling rates. We report accurate period determinations for the main peak at 489.334 48 s and two other normal modes using data from the Whole Earth Telescope (WET) observations of 1995 and 2009. In 2009, we detected a new pulsation mode and the main pulsation mode exhibited substantial change in its amplitude compared to all previous observations. We were able to estimate the star’s rotation period, of 8.9 h, and discuss a possible determination of the rate of period change of (2.0 ± 0.9) × 10−13 s s-ˡ, the first such estimate for a DBV

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly