SDSL-ESR-based protein structure characterization

As proteins are key molecules in living cells, knowledge about their structure can provide important insights and applications in science, biotechnology, and medicine. However, many protein structures are still a big challenge for existing high-resolution structure-determination methods, as can be seen in the number of protein structures published in the Protein Data Bank. This is especially the case for less-ordered, more hydrophobic and more flexible protein systems. The lack of efficient methods for structure determination calls for urgent development of a new class of biophysical techniques. This work attempts to address this problem with a novel combination of site-directed spin labelling electron spin resonance spectroscopy (SDSL-ESR) and protein structure modelling, which is coupled by restriction of the conformational spaces of the amino acid side chains. Comparison of the application to four different protein systems enables us to generalize the new method and to establish a general procedure for determination of protein structur

The Readability Of Instructions For Income Taxation In The Western States

The taxation of individual income represents an important revenue source for many states. The taxes are determined by a self-assessed tax system from information that is the declaration of the taxpayer in an annual return. State taxation agencies facilitate compliance by furnishing individuals with the necessary forms and instruction materials needed to prepare the self-assessed tax return. This study reports on a readability assessment of the income tax instructions for states in the West. The results find improvements in the readability averages for tax instructions between 1990 and 2005, but in absolute terms the instructions for Western states are difficult to read. State income tax instructions have a readability level that currently exceeds the educational attainment level of nearly half the adult population in the West

The Atmospheric Surface Layer over Dronning Maud Land, Antarctica

Master's Thesis in Meteorology and OceanographyGEOF399MAMN-GEO

The Dearth of Neutral Hydrogen in Galactic Dwarf Spheroidal Galaxies

We present new upper limits on the neutral hydrogen (H i) content within the stellar half-light ellipses of 15 Galactic dwarf spheroidal galaxies (dSphs), derived from pointed observations with the Green Bank Telescope (GBT) as well as Arecibo L-band Fast ALFA survey and Galactic All-Sky Survey data. All of the limits are more stringent than previously reported values, and those from the GBT improve upon constraints in the literature by a median factor of 23. Normalizing by V-band luminosity LV and dynamical mass Mdyn, we find and , irrespective of location in the Galactic halo. Comparing these relative H i contents to those of the Local Group and nearby neighbor dwarfs compiled by McConnachie, we find that the Galactic dSphs are extremely gas-poor. Our H i upper limits therefore provide the clearest picture yet of the environmental dependence of the H i content in Local Volume dwarfs. If ram pressure stripping explains the dearth of H i in these systems, then orbits in a relatively massive Milky Way are favored for the outer halo dSph Leo I, while Leo II and Canes Venatici I have had a pericentric passage in the past. For Draco and Ursa Minor, the interstellar medium mass that should accumulate through stellar mass loss in between pericentric passages exceeds by a factor of ~30. In Ursa Minor, this implies that either this material is not in the atomic phase, or that another mechanism clears the recycled gas on shorter timescales

Role of the national energy system modelling in the process of the policy development

Strategic planning and decision making, nonetheless making energy policies and strategies, is very extensive process and has to follow multiple and often contradictory objectives. During the preparation of the new Slovenian Energy Programme proposal, complete update of the technology and sector oriented bottom up model of Reference Energy and Environmental System of Slovenia (REES-SLO) has been done. During the redevelopment of the REES-SLO model trade-off between the simulation and optimisation approach has been done, favouring presentation of relations between controls and their effects rather than the elusive optimality of results which can be misleading for small energy systems. Scenario-based planning was integrated into the MESAP (Modular Energy System Analysis and Planning) environment, allowing integration of past, present and planned (calculated) data in a comprehensive overall system. Within the paper, the main technical, economic and environmental characteristics of the Slovenian energy system model REES-SLO are described. This paper presents a new approach in modelling relatively small energy systems which goes beyond investment in particular technologies or categories of technology and allows smooth transition to low carbon economy. Presented research work confirms that transition from environment unfriendly fossil fuelled economy to sustainable and climate friendly development requires a new approach, which must be based on excellent knowledge of alternative possibilities of development and especially awareness about new opportunities in exploitation of energy efficiency and renewable energy sources

The Role of Teamwork in the Creation of Open Educational Resources for Closing SDG-Related Knowledge Gaps

The Sustainable Development Goals (SDGs) with their interconnected targets can only be achieved if their interactions are seriously taken into consideration. Education for sustainability is crucial for raising awareness of different stakeholders about the SDGs and their interactions. The paper discusses how teamwork creation of Open Educational Resources (OER), with their specific benefits, can contribute to closing knowledge gaps about the SDGs and their interactions. The example of OE4BW (Open Education for a Better World) international mentoring program and its energy-related hub, called the SDG7 Hub is presented and discussed, and its future development, targeting the creation of OER to close the SDG7-related knowledge gaps, is suggested. The resulting guidelines are generalized to provide a further increase of OER’s contribution to the achievement of SDGs

Return to Sender: The need to re-address patient antibiotic allergy labels in Australia and New Zealand

BACKGROUND: Antibiotic allergies are frequently reported and have significant impacts upon appropriate prescribing and clinical outcomes. We surveyed infectious diseases physicians, allergists, clinical immunologists and hospital pharmacists to evaluate antibiotic allergy knowledge and service delivery in Australia and New Zealand. METHODS: An online multi-choice questionnaire was developed and endorsed by representatives of the Australasian Society of Clinical Immunology and Allergy (ASCIA), Australasian Society of Infectious Diseases (ASID) and Society of Hospital Pharmacists Australia (SHPA). The 37-item survey was distributed in April 2015 to members of ASCIA, ASID, SHPA and Royal Australasian College of Physicians. RESULTS: Of 277 respondents, 94% currently use or would utilise antibiotic allergy testing (AAT) and reported seeing up to 10 patients/week labelled as antibiotic-allergic. Forty-two per cent were not aware of or did not have AAT available. Most felt that AAT would aid antibiotic selection, antibiotic appropriateness and antimicrobial stewardship (79%, 69% and 61%, respectively). Patients with histories of immediate hypersensitivity were more likely to be referred than those with delayed hypersensitivities (76% vs. 41%, p=0.0001). Lack of specialist physicians (20%) and personal experience (17%) were barriers to service delivery. A multidisciplinary approach was the preferred AAT model (53%). Knowledge gaps were identified, with the majority over-estimating rates of penicillin/cephalosporin (78%), penicillin/carbapenem (57%) and penicillin/monobactam (39%) cross-reactivity. CONCLUSIONS: A high burden of antibiotic allergy labelling and demand for AAT is complicated by a relative lack availability or awareness of AAT services in Australia and New Zealand. Antibiotic allergy education and deployment of AAT, accessible to community and hospital-based clinicians, may improve clinical decisions and reduce antibiotic allergy impacts. A collaborative approach involving ID physicians, pharmacists and allergists/immunologists is required