Changes of fatty acid metabolism and oxidative phosphorylation of rat liver mitochondria during 3'-Me-DAB feeding

Respiration, activity of oleate oxidation and composition of the total fatty acids of rat liver were investigated in 3'-Me-DAB feeding. 1. Oxidative phosphorylation of rat liver mitochondria decreased temporarily at relatively earlier stages (about 2 to 3 weeks) in 3'-Me-DAB feeding. 2. The activity of oleate oxidation of rat liver mitochondria decreased rapidly to about one third of that in control groups after the start of 3'-Me-DAB feeding. 3. In the composition of the total fatty acids of rat liver, the proportion of oleic acid increased in 3'-Me-DAB groups. 4. Unknown octadecamonoenoic acid was observed in liver mitochondria of rat fed on 3'-Me-DAB. 5. Proportions of oleic and palmitoleic acids in liver tumors and mitochondria of liver tumors induced by 3'-Me-DAB feeding increased remarkably in contrast with decrease in those of palmitic and eicosapolyenoic acids. 6. A possibility was discussed about how higher level of oleate in the liver cells in azo dye feeding may be concerned with the tumor induction.</p

Wide-area remote-sensing system of pollution and gas dispersal by near-infrared absorption based on low-loss optical fiber network

An all optical remote sensing system utilizing long distance, ultralow loss optical fiber networks is studied and discussed for near infrared absorption measurements of combustible and/or explosive gases such as CH4 and C3H8 in our environment, including experimental results achieved in a diameter more than 20 km. The use of a near infrared wavelength range is emphasized

Nucleic acids and protein synthesis in cancer cell mitochondria. I. Nucleic acids in rat hepatoma mitochondria

The contents of nucleic acids in rat liver and hepatoma mitochondria and the physico-chemical properties on DNA's isolated from these mitochondria were comparatively investigated. The results are briefly summarized as follows. 1. The contents of DNA and RNA per mg protein of the hepatoma cell mitochondria were about 10 and 2 to 4 times higher than those of rat liver mitochondria, respectively. 2. The &#955; max. and &#955;min. values of DNA isolated from the hepatoma mitochondria were 257 m&#956; and 231 m&#956;, respectively and those of DNA isolated from the nuclei were 259 m&#956; and 233 m&#956;, respectively, in saline-citrate, pH 7.0. 3. Three fractions of mitochondrial DNA were obtained by the sucrose density gradient and these DNA fractions corresponded, probably, to about 30 S, and 20 S and 14 S DNA's. 4. There was little difference in base compositions between nuclear and mitochondrial DNA's of the hepatoma cells. 5. The degree of hybridization between the nuclear and mitochondrial DNA's of the hepatoma cells was almost the same as that between the nuclear and nuclear DNA's of the hepatoma cells, and somewhat higher than that between the nuclear DNA of rat liver and the nuclear DNA of hepatoma cells. 6. &#34;Highly twisted&#34; circular, &#34;open&#34; circular and linear forms were observed in the DNA preparations of the hepatoma mitochondria. The average values of contour lengths of rat liver and the hepatoma DNA's observed at high frequency were 5.3 &#956; and 4.5 &#956;. 7. A discussion was made on the relation between the genetic informations of mitochondrial DNA and the formation of a mitochondrion in rat liver and the hepatoma cells.</p

The net reproduction rate and the type-reproduction number in multiregional demography

In order to study effects of migration on demographic changes of multiregional populations, multiregional population modelling is a useful traditional tool. Although multiregional mathematical demography has been extensively explored since the beginning of the 1970s, its key concept, the multiregional net reproduction rate, has been long neglected. In this review, we focus on a multiregional stable population system and elaborate the definition of the multiregional net reproduction rate. Next we introduce the type-reproduction number from mathematical epidemiology and show that it becomes a useful index to formulate a simple control relation for a multiregional population. Mathematical ideas presented here will help us to reconsider multiregional mathematical demography, which is a useful theoretical framework to study effects of interregional migration on population dynamics and composition.