10 research outputs found
The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries.
The docking protein p140Cap negatively regulates tumour cell features. Its relevance on
breast cancer patient survival, as well as its ability to counteract relevant cancer signalling
pathways, are not fully understood. Here we report that in patients with ERBB2-amplified
breast cancer, a p140Cap-positive status associates with a significantly lower probability of
developing a distant event, and a clear difference in survival. p140Cap dampens ERBB2-
positive tumour cell progression, impairing tumour onset and growth in the NeuT mouse
model, and counteracting epithelial mesenchymal transition, resulting in decreased metastasis
formation. One major mechanism is the ability of p140Cap to interfere with ERBB2-
dependent activation of Rac GTPase-controlled circuitries. Our findings point to a specific role
of p140Cap in curbing the aggressiveness of ERBB2-amplified breast cancers and suggest
that, due to its ability to impinge on specific molecular pathways, p140Cap may represent a
predictive biomarker of response to targeted anti-ERBB2 therapies
Efficacy of a new technique - INtubate-RECruit-SURfactant-Extubate - "IN-REC-SUR-E" - in preterm neonates with respiratory distress syndrome: Study protocol for a randomized controlled trial
Background: Although beneficial in clinical practice, the INtubate-SURfactant-Extubate (IN-SUR-E) method is not successful in all preterm neonates with respiratory distress syndrome, with a reported failure rate ranging from 19 to 69 %. One of the possible mechanisms responsible for the unsuccessful IN-SUR-E method, requiring subsequent re-intubation and mechanical ventilation, is the inability of the preterm lung to achieve and maintain an "optimal" functional residual capacity. The importance of lung recruitment before surfactant administration has been demonstrated in animal studies showing that recruitment leads to a more homogeneous surfactant distribution within the lungs. Therefore, the aim of this study is to compare the application of a recruitment maneuver using the high-frequency oscillatory ventilation (HFOV) modality just before the surfactant administration followed by rapid extubation (INtubate-RECruit-SURfactant-Extubate: IN-REC-SUR-E) with IN-SUR-E alone in spontaneously breathing preterm infants requiring nasal continuous positive airway pressure (nCPAP) as initial respiratory support and reaching pre-defined CPAP failure criteria. Methods/design: In this study, 206 spontaneously breathing infants born at 24+0-27+6 weeks' gestation and failing nCPAP during the first 24 h of life, will be randomized to receive an HFOV recruitment maneuver (IN-REC-SUR-E) or no recruitment maneuver (IN-SUR-E) just prior to surfactant administration followed by prompt extubation. The primary outcome is the need for mechanical ventilation within the first 3 days of life. Infants in both groups will be considered to have reached the primary outcome when they are not extubated within 30 min after surfactant administration or when they meet the nCPAP failure criteria after extubation. Discussion: From all available data no definitive evidence exists about a positive effect of recruitment before surfactant instillation, but a rationale exists for testing the following hypothesis: a lung recruitment maneuver performed with a step-by-step Continuous Distending Pressure increase during High-Frequency Oscillatory Ventilation (and not with a sustained inflation) could have a positive effects in terms of improved surfactant distribution and consequent its major efficacy in preterm newborns with respiratory distress syndrome. This represents our challenge. Trial registration: ClinicalTrials.gov identifier: NCT02482766. Registered on 1 June 2015
withdrawn 2017 hrs ehra ecas aphrs solaece expert consensus statement on catheter and surgical ablation of atrial fibrillation
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Glycoconjugate distribution in gastric fundic mucosa of Umbrina cirrosa L. revealed by lectin histochemistry
The stomach of adult shi drum Umbrina cirrosa was investigated using a battery of nine horseradish peroxidase-conjugated lectins combined with enzymatic treatment, in order to distinguish glycoconjugate sugar residues. Epithelial cells showed the presence of galactosyl(β1→4)N- acetylglucosamine, mannose, N-acetylgalactosamine, N-acetylglucosamine, fucose and sialic acid-galactosyl(β1→3)N-acetylgalactosamine residues. Gastric pits had similar sugar residues with the exception of N-acetylgalactosamine which was less diffused. Gastric glands were characterized by the presence of glycoconjugates containing galactosyl(β1→3)N- acetylgalactosamine, N-acetylglucosamine, galactosyl(β1→4) N-acetylglucosamine, N-acetylgalactosamine and a small amount of sialic acid linked to N-acetylgalactosamine
Comportamiento prooxidante y antioxidante de hierbas aromáticas en queso
Los quesos de cabra, con mejores cualidades funcionales provienen de leche de cabras en pastoreo, esto obedece al mayor contenido de ácidos grasos ω-3 y ω-6 en una relación óptima para la salud, sumado a un menor contenido de grasa y colesterol. Saborizar quesos con especias, otorga beneficios sensoriales y saludables al producto. Las especias son usadas para realzar el flavor de los alimentos además presentan beneficios antioxidantes. Sin embargo, se reportaron evidencias del doble papel de los flavonoides como antioxidantes o prooxidantes en función de la concentración de uso. El objetivo del trabajo es evaluar el efecto de distintas concentraciones de especias aromáticas con capacidad antioxidante, frente a las reacciones de deterioro que ocurren en el almacenamiento de quesos de cabra. Se estudió la actividad antirradicalaria (AAR) de 18 especias. Para evaluar la actividad antioxidante (AA0) se usó la técnica de desaparición de un radical libre estable el 2,2-difenil-1-picril hidrazilo (DPPH) y se calculó la AAR porcentual. Definido el queso base estándar (control), se ensayaron tres variedades con especies, elegidas de entre las de mayor AAR (orégano, romero y tomillo) y se adicionaron con las concentraciones 0,4 – 1 % p/p. Estos, fueron madurados 30 días y almacenados 7 meses a 4 °C. Se analizaron cada 4 semanas. Se realizaron pruebas sensoriales de aceptabilidad con 60 consumidores de queso (condición), usando planillas, con escala hedónica. Se trabajó sobre los lípidos extraídos de los quesos. El rancidez y deterioro fueron evaluados con análisis de acidez; índice peróxido y sustancias reactivas al ácido TBA. Los resultados se compararon con el comportamiento sensorial del producto en el tiempo de almacenamiento. En los quesos adicionados al 1 %, la acidez se incrementó con el almacenamiento. El máximo valor corresponde al queso con orégano (3,72 % de ac. láctico a los 126 días). En el control, la acidez inicial fue inferior y alcanzó 1,05 g de ác. láctico % a los 166 días. El IP más alto corresponde a los quesos con orégano al 1% , resultado que se contrapone a la actividad antirradicalaria de esta especia. Al 0,4 %, tanto el IP como el número de TBA son menores en quesos adicionados, respecto del control. En los quesos de cabra analizados las especias agregadas al 1% ejercieron acción prooxidante, mientras que al ser agregadas al 0,4% la acción es antioxidante, lo que indica la importancia de la selección de concentraciones adecuadas.Goat cheeses with better functional qualities, due to greater fatty acid content ω 3 and ω 6, come from free-range goat milk in an optimal relation for health, besides having a smaller content of fat and cholesterol. The spices, which present antioxidant benefits, are used to heighten the flavor of foods. Nevertheless, evidence of the double role of the flavonoids as antioxidant or prooxidant was reported based on their use concentration. Flavoring cheeses with spices gives sensory and healthy benefits to the product. The objective of the present work is to evaluate the effect of different aromatic spice concentrations with antioxidant capacity, as opposed to the deterioration reactions that happen in the goat cheese storage. The antiradical capacity (ARA) of 18 spices was studied. In order to evaluate the Antioxidant activity (AOA) the technique of disappearance of a stable free radical was used and the 2,2-diphenyl-2-picrylhydrazyl (DPPH) and the percentage ARA were calculated as well. Once the standard basic cheese (control) was defined, we tried three varieties with species, chosen from among those of greater AAR (oregano, rosemary and thyme) and were added with 0,4 - 1% p/p concentrations. These ripened in 30 days and were stored for 7 months at 4 °C. Every 4 weeks they were analyzed. Sensory tests of acceptability with 60 cheese consumers were carried out (condition), using checklists, with the hedonic scale. The work was performed on lipids extracted of cheeses according to Bligh Dyer method. The rancid flavor and deterioration were evaluated with the analysis of acidity; peroxide index (PI), and reactive substances to TBA acid. The results were compared with the sensory behavior of the product during the time of storage. All the assays were produced in triplicate. The data were put under the descriptive statistical and variance analysis. In cheeses, to which spices were added to 1%, the acidity increased with the storage. The maximum value was the cheese with oregano (3.72% of ac. lactic to the 126 days). In the control, the initial acidity was inferior and reached 1.05 g % of lactic acid to the 166 days. The PI in cheeses to 1% with oregano was higher, result that is opposed to the antiradical activity of this spice. To 0.4%, both the PI and the number of TBA are smaller in added cheeses, with respect to the control. In goat cheeses the spices added to 1% exert prooxidating action. However, to 0.4% it is AAO, which indicates the importance of the selection of suitable concentrations. Key words: antioxidant capacity - prooxidant- spices - goat cheese – rancid flavo
Efficacy of a new technique - INtubate-RECruit-SURfactant-Extubate - "IN-REC-SUR-E" - in preterm neonates with respiratory distress syndrome: study protocol for a randomized controlled trial.
BACKGROUND:
Although beneficial in clinical practice, the INtubate-SURfactant-Extubate (IN-SUR-E) method is not successful in all preterm neonates with respiratory distress syndrome, with a reported failure rate ranging from 19 to 69 %. One of the possible mechanisms responsible for the unsuccessful IN-SUR-E method, requiring subsequent re-intubation and mechanical ventilation, is the inability of the preterm lung to achieve and maintain an "optimal" functional residual capacity. The importance of lung recruitment before surfactant administration has been demonstrated in animal studies showing that recruitment leads to a more homogeneous surfactant distribution within the lungs. Therefore, the aim of this study is to compare the application of a recruitment maneuver using the high-frequency oscillatory ventilation (HFOV) modality just before the surfactant administration followed by rapid extubation (INtubate-RECruit-SURfactant-Extubate: IN-REC-SUR-E) with IN-SUR-E alone in spontaneously breathing preterm infants requiring nasal continuous positive airway pressure (nCPAP) as initial respiratory support and reaching pre-defined CPAP failure criteria.
METHODS/DESIGN:
In this study, 206 spontaneously breathing infants born at 24(+0)-27(+6) weeks' gestation and failing nCPAP during the first 24 h of life, will be randomized to receive an HFOV recruitment maneuver (IN-REC-SUR-E) or no recruitment maneuver (IN-SUR-E) just prior to surfactant administration followed by prompt extubation. The primary outcome is the need for mechanical ventilation within the first 3 days of life. Infants in both groups will be considered to have reached the primary outcome when they are not extubated within 30 min after surfactant administration or when they meet the nCPAP failure criteria after extubation.
DISCUSSION:
From all available data no definitive evidence exists about a positive effect of recruitment before surfactant instillation, but a rationale exists for testing the following hypothesis: a lung recruitment maneuver performed with a step-by-step Continuous Distending Pressure increase during High-Frequency Oscillatory Ventilation (and not with a sustained inflation) could have a positive effects in terms of improved surfactant distribution and consequent its major efficacy in preterm newborns with respiratory distress syndrome. This represents our challenge.
TRIAL REGISTRATION:
ClinicalTrials.gov identifier: NCT02482766 . Registered on 1 June 2015