Resistivity during cycle loading of fine grain heat affected zone (HAZ) of 17CrNiMo7 steel prepared into laboratory furnace

Structural components which are loaded in cyclic load mostly fail due to fatigue. The fatigue strength of metals as well as weld joint regions in metals depends on mechanical properties especially hardness, the size of defect and the size of grains. Resistivity of the fine grain HAZ prepared against fatigue crack initiation will be explained in this article. Samples of material with martensitic fine grain microstructure were prepared by laboratory furnace by proper thermal treatment. Mechanical properties of fine grain heat affected zone and its microstructure were investigated. Special attention was given to behavior of fine grain during the cycle loading under stress concentration. Stress concentration was similar to the one in real welds. The S-N curve and the fatigue limit were determined. The Paris curve and the threshold for crack propagating were also determined

Chemical composition of the essential oil of Brachyapium dichotomum (L.) Maire

The essential oil obtained by hydrodistillation from the aerial parts of Brachyapium dichotomum (L.) Maire (Apiaceae), was analyzed by GC-MS for the first time. Sixty-two compounds were detected, accounting for 96.1% of the total oil, which is characterized by a high content of hydrocarbons derivatives of mono- and sesquiterpenes (80.3%). The main constituents were α-pinene (36.5%), germacrene D (17.9%), dill apiole (6.7%) and myristicin (3.5%)

Glutamate induces autophagy via the two-pore channels in neural cells

NAADP (nicotinic acid adenine dinucleotide phosphate) has been proposed as a second messenger for glutamate in neuronal and glial cells via the activation of the lysosomal Ca2+ channels TPC1 and TPC2. However, the activities of glutamate that are mediated by NAADP remain unclear. In this study, we evaluated the effect of glutamate on autophagy in astrocytes at physiological, non-toxic concentration. We found that glutamate induces autophagy at similar extent as NAADP. By contrast, the NAADP antagonist NED-19 or SiRNA-mediated inhibition of TPC1/2 decreases autophagy induced by glutamate, confirming a role for NAADP in this pathway. The involvement of TPC1/2 in glutamate-induced autophagy was also confirmed in SHSY5Y neuroblastoma cells. Finally, we show that glutamate leads to a NAADP-dependent activation of AMPK, which is required for autophagy induction, while mTOR activity is not affected by this treatment. Taken together, our results indicate that glutamate stimulates autophagy via NAADP/TPC/AMPK axis, providing new insights of how Ca2+ signalling glutamate-mediated can control the cell metabolism in the central nervous system

A Hybrid Sensor Based Backstepping Control Approach with its application to Fault-Tolerant Flight Control

Recently, an incremental type sensor based backstepping (SBB) control approach, based on singular perturbation theory and Tikhonov’s theorem, has been proposed. This Lyapunov function based method uses measurements of control variables and less model knowledge, and it is not susceptible to the model uncertainty caused by fault scenarios. In this paper, the SBB method has been implemented on a fixed wing aircraft with its focus on handling structural changes caused by damages. A new hybrid autopilot flight controller has been developed for a Boeing 747-200 aircraft after combining nonlinear dynamic inversion (NDI) with SBB control approach. Two benchmarks for fault tolerant flight control (FTFC), named rudder runaway and engine separation, are employed to evaluate the proposed method. The simulation results show that the proposed control approach leads to a zero tracking-error performance in nominal condition and guarantees the stability of the closed-loop system under failures as long as the reference commands are located in the safe flight envelope

Preliminary petrological inferences on the high-grade metamorphic rocks exhumed by the South Rif Thrust, Prerif, Northern Morocco

The exhumation of deep crustal rocks along major shear zones is common, yet a highly debated subject, particularly when occurring during recent tectonic events. This is the case of the South Rif Thrust (SRT), a significant shear zone analogous to those described at the Betic Cordillera in Spain. The SRT separates two major geodynamic domains in Northern Morocco: a) the Prerif, to the North, mostly composed of Miocenic sedimentary units on top of a stratigraphic sequence continuously deposited since the Triassic; and b) the Western Meseta, to the South, mostly composed of Paleozoic metasedimentary units, correlated with the Iberian Variscan Belt [1]. Associated to the SRT, and exhumed by its activity, there is a dismembered and exotic high-grade metamorphic belt representative of the middle and lower crust. Also in this region, several thermo-mineral waters occur, whose deep circulation can be traced back to the SRT [2]. Detailed geological mapping, structural, stratigraphic and petrographic analyses on this dismembered and highly deformed exotic sequence reveal the presence of low- to high-grade metasediments (including migmatites and felsic granulites), but mostly high-grade metabasic and basic rocks, including amphibolites, mafic granulites and gabbros. Preliminary geothermobarometry in the mafic granulites provides an important characterization of the infra-crustal conditions of the pre-Alpine geodynamics and of the activity and exhumation along the SRT since the Miocene: a) the mafic granulites endured M1 metamorphic peak conditions of T = 1030 ºC at P = 8.5 kbar, which is consistent with typical conductive continental crust geothermal gradients (~30 ºC.km-1); b) M2 retrogression occurred by near isothermal decompression at T = 820 ºC and P = 3.5 kbar, implying an initial vertical uplift of >18 km of the granulite-facies rocks to very shallow levels; c) during this period, the geothermal gradient in the region surpassed 60 ºC.km-1; d) exhumation and retrogression continued by almost isobaric cooling at T < 750 ºC and P = 1.7 – 3.0 kbar with an M3 amphibolitization of the granulites after late water inflow. The overall metamorphic evolution of these deep crustal rocks is compatible with a clockwise P-T path, involving initial fast tectonic exhumation, followed by thermal readjustment to shallower levels. This is consistent with the currently observed geothermal gradients in the area (≤ 42 ºC.km-1) [2] which may still be a reflection of the events during the Miocene. These petrological constrains on the tectonic processes associated with the exhumation of this lower crust segment and the activity of the SRT during the closure of the Alboran Basin are key to understanding the circulation of deep hot waters, which are an important part of the economy of this region in Northern Morocco.publishe

QAUST: Protein Function Prediction Using Structure Similarity, Protein Interaction, and Functional Motifs

The number of available protein sequences in public databases is increasing exponentially. However, a significant percentage of these sequences lack functional annotation, which is essential for the understanding of how biological systems operate. Here, we propose a novel method, Quantitative Annotation of Unknown STructure (QAUST), to infer protein functions, specifically Gene Ontology (GO) terms and Enzyme Commission (EC) numbers. QAUST uses three sources of information: structure information encoded by global and local structure similarity search, biological network information inferred by protein–protein interaction data, and sequence information extracted from functionally discriminative sequence motifs. These three pieces of information are combined by consensus averaging to make the final prediction. Our approach has been tested on 500 protein targets from the Critical Assessment of Functional Annotation (CAFA) benchmark set. The results show that our method provides accurate functional annotation and outperforms other prediction methods based on sequence similarity search or threading. We further demonstrate that a previously unknown function of human tripartite motif-containing 22 (TRIM22) protein predicted by QAUST can be experimentally validated

A cyclopalladated complex interacts with mitochondrial membrane thiol-groups and induces the apoptotic intrinsic pathway in murine and cisplatin-resistant human tumor cells

<p>Abstract</p> <p>Background</p> <p>Systemic therapy for cancer metastatic lesions is difficult and generally renders a poor clinical response. Structural analogs of cisplatin, the most widely used synthetic metal complexes, show toxic side-effects and tumor cell resistance. Recently, palladium complexes with increased stability are being investigated to circumvent these limitations, and a biphosphinic cyclopalladated complex {Pd₂[<it>S_(-)</it>C², N-dmpa]₂(μ-dppe)Cl₂} named C7a efficiently controls the subcutaneous development of B16F10-Nex2 murine melanoma in syngeneic mice. Presently, we investigated the melanoma cell killing mechanism induced by C7a, and extended preclinical studies.</p> <p>Methods</p> <p>B16F10-Nex2 cells were treated <it>in vitro </it>with C7a in the presence/absence of DTT, and several parameters related to apoptosis induction were evaluated. Preclinical studies were performed, and mice were endovenously inoculated with B16F10-Nex2 cells, intraperitoneally treated with C7a, and lung metastatic nodules were counted. The cytotoxic effects and the respiratory metabolism were also determined in human tumor cell lines treated <it>in vitro </it>with C7a.</p> <p>Results</p> <p>Cyclopalladated complex interacts with thiol groups on the mitochondrial membrane proteins, causes dissipation of the mitochondrial membrane potential, and induces Bax translocation from the cytosol to mitochondria, colocalizing with a mitochondrial tracker. C7a also induced an increase in cytosolic calcium concentration, mainly from intracellular compartments, and a significant decrease in the ATP levels. Activation of effector caspases, chromatin condensation and DNA degradation, suggested that C7a activates the apoptotic intrinsic pathway in murine melanoma cells. In the preclinical studies, the C7a complex protected against murine metastatic melanoma and induced death in several human tumor cell lineages <it>in vitro</it>, including cisplatin-resistant ones. The mitochondria-dependent cell death was also induced by C7a in human tumor cells.</p> <p>Conclusions</p> <p>The cyclopalladated C7a complex is an effective chemotherapeutic anticancer compound against primary and metastatic murine and human tumors, including cisplatin-resistant cells, inducing apoptotic cell death via the intrinsic pathway.</p

Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set

We report a measurement of the bottom-strange meson mixing phase \beta_s using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays in which the quark-flavor content of the bottom-strange meson is identified at production. This measurement uses the full data set of proton-antiproton collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity. We report confidence regions in the two-dimensional space of \beta_s and the B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2, -1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in agreement with the standard model expectation. Assuming the standard model value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +- 0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +- 0.009 (syst) ps, which are consistent and competitive with determinations by other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012