A human biomonitoring (HBM) Global Registry Framework: Further advancement of HBM research following the FAIR principles.

Data generated by the rapidly evolving human biomonitoring (HBM) programmes are providing invaluable opportunities to support and advance regulatory risk assessment and management of chemicals in occupational and environmental health domains. However, heterogeneity across studies, in terms of design, terminology, biomarker nomenclature, and data formats, limits our capacity to compare and integrate data sets retrospectively (reuse). Registration of HBM studies is common for clinical trials; however, the study designs and resulting data collections cannot be traced easily. We argue that an HBM Global Registry Framework (HBM GRF) could be the solution to several of challenges hampering the (re)use of HBM (meta)data. The aim is to develop a global, host-independent HBM registry framework based on the use of harmonised open-access protocol templates from designing, undertaking of an HBM study to the use and possible reuse of the resulting HBM (meta)data. This framework should apply FAIR (Findable, Accessible, Interoperable and Reusable) principles as a core data management strategy to enable the (re)use of HBM (meta)data to its full potential through the data value chain. Moreover, we believe that implementation of FAIR principles is a fundamental enabler for digital transformation within environmental health. The HBM GRF would encompass internationally harmonised and agreed open access templates for HBM study protocols, structured web-based functionalities to deposit, find, and access harmonised protocols of HBM studies. Registration of HBM studies using the HBM GRF is anticipated to increase FAIRness of the resulting (meta)data. It is also considered that harmonisation of existing data sets could be performed retrospectively. As a consequence, data wrangling activities to make data ready for analysis will be minimised. In addition, this framework would enable the HBM (inter)national community to trace new HBM studies already in the planning phase and their results once finalised. The HBM GRF could also serve as a platform enhancing communication between scientists, risk assessors, and risk managers/policy makers. The planned European Partnership for the Assessment of Risk from Chemicals (PARC) work along these lines, based on the experience obtained in previous joint European initiatives. Therefore, PARC could very well bring a first demonstration of first essential functionalities within the development of the HBM GRF

Solubilization of Proteins in 2DE: An Outline

Protein solubilization for two-dimensional electrophoresis (2DE) has to break molecular interactions to separate the biological contents of the material of interest into isolated and intact polypeptides. This must be carried out in conditions compatible with the first dimension of 2DE, namely isoelectric focusing. In addition, the extraction process must enable easy removal of any nonprotein component interfering with the isoelectric focusing. The constraints brought in this process by the peculiar features of isoelectric focusing are discussed, as well as their consequences in terms of possible solutions and limits for the solubilization process

Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set

We report a measurement of the bottom-strange meson mixing phase \beta_s using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays in which the quark-flavor content of the bottom-strange meson is identified at production. This measurement uses the full data set of proton-antiproton collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity. We report confidence regions in the two-dimensional space of \beta_s and the B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2, -1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in agreement with the standard model expectation. Assuming the standard model value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +- 0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +- 0.009 (syst) ps, which are consistent and competitive with determinations by other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012

Development and multicenter validation of a multiparametric imaging model to predict treatment response in rectal cancer

Funding Information: This study has received funding from the Dutch Cancer Society (project number 10138). Publisher Copyright: © 2023, The Author(s).Objectives: To develop and validate a multiparametric model to predict neoadjuvant treatment response in rectal cancer at baseline using a heterogeneous multicenter MRI dataset. Methods: Baseline staging MRIs (T2W (T2-weighted)-MRI, diffusion-weighted imaging (DWI) / apparent diffusion coefficient (ADC)) of 509 patients (9 centres) treated with neoadjuvant chemoradiotherapy (CRT) were collected. Response was defined as (1) complete versus incomplete response, or (2) good (Mandard tumor regression grade (TRG) 1–2) versus poor response (TRG3-5). Prediction models were developed using combinations of the following variable groups: (1) Non-imaging: age/sex/tumor-location/tumor-morphology/CRT-surgery interval (2) Basic staging: cT-stage/cN-stage/mesorectal fascia involvement, derived from (2a) original staging reports, or (2b) expert re-evaluation (3) Advanced staging: variables from 2b combined with cTN-substaging/invasion depth/extramural vascular invasion/tumor length (4) Quantitative imaging: tumour volume + first-order histogram features (from T2W-MRI and DWI/ADC) Models were developed with data from 6 centers (n = 412) using logistic regression with the Least Absolute Shrinkage and Selector Operator (LASSO) feature selection, internally validated using repeated (n = 100) random hold-out validation, and externally validated using data from 3 centers (n = 97). Results: After external validation, the best model (including non-imaging and advanced staging variables) achieved an area under the curve of 0.60 (95%CI=0.48–0.72) to predict complete response and 0.65 (95%CI=0.53–0.76) to predict a good response. Quantitative variables did not improve model performance. Basic staging variables consistently achieved lower performance compared to advanced staging variables. Conclusions: Overall model performance was moderate. Best results were obtained using advanced staging variables, highlighting the importance of good-quality staging according to current guidelines. Quantitative imaging features had no added value (in this heterogeneous dataset). Clinical relevance statement: Predicting tumour response at baseline could aid in tailoring neoadjuvant therapies for rectal cancer. This study shows that image-based prediction models are promising, though are negatively affected by variations in staging quality and MRI acquisition, urging the need for harmonization. Key Points: This multicenter study combining clinical information and features derived from MRI rendered disappointing performance to predict response to neoadjuvant treatment in rectal cancer. Best results were obtained with the combination of clinical baseline information and state-of-the-art image-based staging variables, highlighting the importance of good quality staging according to current guidelines and staging templates. No added value was found for quantitative imaging features in this multicenter retrospective study. This is likely related to acquisition variations, which is a major problem for feature reproducibility and thus model generalizability.Peer reviewe

Looking beyond the spots: inspiring the public to record ladybirds

The Coccinellidae Recording Scheme, the UK’s scheme for mapping coccinellid distributions, was launched in 1968 and was led by Michael Majerus from the 1980s. In the early years participants tended to be experienced naturalists, so to draw others in, Mike set up a very successful offshoot, the Cambridge Ladybird Survey (CLS), a public outreach survey that ran from 1984 to 1994. The CLS generated a huge amount of data and made a significant contribution to Mike’s New Naturalist ‘Ladybirds’ book (1994). When Harmonia axyridis arrived in the UK in late 2004, Mike realised the potential for involving the public in a unique opportunity to study the spread of an invasive animal from the start of the invasion process. Thus the Harlequin Ladybird Survey was launched in early 2005 as one of the first online wildlife surveys in the UK. Over 26,000 online records have been received, enabling a study of unprecedented detail. Mike was very adept at using the media to convey his message. His expertise extended to the Lepidoptera (including evolutionary studies of the peppered moth Biston betularia) and presidency of the Amateur Entomologists' Society, enabling further outreach opportunities

Ladybirds (Coccinellidae) of Britain and Ireland

The ladybird (Coccinellidae) family comprises a diverse group of beetles. There are 47 species of Coccinellidae resident in Britain and Ireland. Some species are brightly coloured and these are colloquially termed “ladybirds”. Others are small and inconspicuous, although these, on close inspection, are just as attractive as their charismatic counterparts. In this atlas, we describe the distribution of ladybirds in Britain, Ireland, the Isle of Man and the Channel Islands, using data collated through the Biological Records Centre Coccinellidae Recording Scheme (including the UK Ladybird Survey) since 1964. Ladybirds are charismatic beetles with fascinating life histories. Their interactions with natural enemies, particularly parasites, are intriguing and we hope that this atlas will encourage further recording of ladybirds and also the natural enemies associated with them. This publication is a celebration of the work of Mike Majerus and the many ladybird recorders he inspired; tens of thousands of people have contributed their records to the UK Ladybird Survey. “...this atlas offers so much more than distribution maps. Using photographs and text it helps with the identification of all ladybird species, from the largest to the smallest, and in all their stages: egg, larva, pupa and adult. There is information on life histories, behaviour, host plants and prey, and details of the enemies of ladybirds, especially their parasites. And it comes at a critical moment in the story of ladybirds in Britain and Ireland.” Roger Hawkins (Ladybirds of Surrey

Ladybirds

This revised and updated edition of Ladybirds provides a succinct but comprehensive and accessible overview of the biology of ladybirds and their parasites, focusing on ecology in an evolutionary context. It provides the latest information, coverage of recent additions to the British list including the harlequin ladybird, and makes suggestions for further research, both short and long term, highlighting gaps in knowledge and showing readers how to get involved with recording and studying ladybirds. It includes updated keys for the identification of ladybirds at late-instar larval and adult stages, and techniques for studying ladybirds and their parasites in both laboratory and field. The authors hope that this book will be a valuable resource, not only for students, from school to university and beyond, but also for anyone with an interest in natural history, whether professional or recreational