A survey on the effect of Cochlodinium.sp bloom on shrimp culture complexes and hatcheries activities in Bushehr Province

The aims of this project was to inform and aware about the red tide condition before entering the algal bloomer in water resources to the hatcheries and farmed shrimp complexes in Bushehr province coastal. Field investigation and sampling have been carried out in the southern of the input water channels of Mond, Delvar and Helleh farmed shrimp complexes, which are located along the coastal of Bushehr Province, from April to December 2011 .The physic-chemical parameters such as pH, salinity and water temperature and also meteorological conditions were measured and recorded on the field. The water sampling has been lunched for determination of nutrients and chlorophyll - a, phyto- and zoo- planktons. No Cochlodinium.sp outbreaks have been detected in the studied area, during the study. The only bloom which reported by a fisherman, was outbreak in offshore of Bushehr province in Khoure Khan on 13th September 2011. The sample which transferred to the Iranian National Shrimp Research Institute was included Alexandrium.sp and its density was 2 million cells per liter. The identified phytoplanktons were belonged to three order of Bacillariophyceae (52.6%) with average density of 10778 cells per liter, Dinophyceae (37.7%) with density of 7731 cells per liter and Cyanophyceae (9.7%) with density of 1980 cells. 12 genera belonged to Dinophyceae , 25 genera of Bacillariophyceae and two genera of Cyanophyceae were observed during the study. The highest density of phytoplankton was recorded in Helleh station by 18374 cells per liter. The maximum density of phytoplankton was at Delvar station by 141120 cells per liter in December. The highest density of the phytoplanktons was belonged to Dinophyceae by 126000 by cells per liter of which the Alexandrium.sp had the density of 124500 cells per liter in August 2011. From the Dinophyceae the Alexandrium.sp with mean density of 20345 cells per liter, Ornithocercus 920 cells and Prorocentrum.sp 820 cells were the predominant species. The identified Zooplankton in were belonged to 8 branches and 19 groups. The highest density of zooplankton was recorded in Helleh station by 1194 no. per liter. Nauplii were the dominant zooplankton groups with an average density of 136.4 no. per liter, Tintinnids 98.7, Cyclopoida 60.8, Calanoida 35.7 and Harpacticoids 14.5 no. per liter . The average of water and air temperature was recorded 29.4 °C and 28.3 °C, respectively. Average of salinity was 41.2 and pH was 8.46. The average of depth in all stations was 5.7 m. The mean concentrations of silicate, nitrate, nitrite, ammonia and total phosphate were 1.99, 0.03, 0.009, 0.14 0.15 ppm, respectively and the average of chlorophyll - a was 0.94 mg.m-3

Analyze the types and methods suitable nutrition in introducing a variety of fish cages in the southern part of Caspian Sea

Regarding the fish farming in cages, taking into account all nutritional requirements appropriate to the needs of aquaculture is essential in order to minimize damage to the environment and achieve maximum growth. Fish nutritionists should be aware about the analysis of ability to digest and absorb nutrients in the diet to prepare and adjust the diet according to the needs of aquaculture. Food must also have appropriate physical properties for food intake with minimal loss of nutrients in the water. Inadequate resources with low quality or energy consumption within food, reduced the growth performance and increased the excretion of nitrogen in the environment. Food must be designed so that the balance of minerals in food will help maintain osmotic pressure within the body of fish in salty water. Food requirements and efficiency of food conversion ratio (FCR) is variable by changing environmental conditions (dissolved oxygen, temperature, water quality, flow rate, light intensity ,day length). The fish of cages are feeding by floating pellets which have the ability to stand in the water column. The floating pellets allows the fish to have sufficient time and opportunity for finding food. But sinking pellets pass rapidly from the cage and are inaccessible to fish. According to the Caspian sea conditions is better the extruded food equipped with Hygenizer to be used that a portion of it remains on the water surface and part of it moves gently down with an immersion mode in the water column (Softly deposited) for fish consumption. The amount, number and time of feeding are important factors to assess the nutritional activities. In general, fish feed from dry food between 2 to 3 percent of their body weight. Food consumption was also varied due to the quality of the food and fish physiological factors such as age, size, stage of life and stress level. Fish food distribution being fed manually or by means of demand, launcher and automatic feeding. The farm managers should pay attention to the things like water flows in the cage, wind, fish appetite, consolidated flows in the cage during food distribution as well as food storage in dry places with proper ventilation system to prevent the growth of fungi and insects activities to prevent loss of food. Also, adding antioxidants to the 100150 ppm when storing dry foods including the important cases particularly in prevention of fish liver lipoidosis disease is considered

Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set

We report a measurement of the bottom-strange meson mixing phase \beta_s using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays in which the quark-flavor content of the bottom-strange meson is identified at production. This measurement uses the full data set of proton-antiproton collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity. We report confidence regions in the two-dimensional space of \beta_s and the B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2, -1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in agreement with the standard model expectation. Assuming the standard model value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +- 0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +- 0.009 (syst) ps, which are consistent and competitive with determinations by other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012

Survey and feasibility study for the introduction of native fish and non- native fish for cage culture in the southern part of Caspian Sea

The aim of this study is the feasibility of introducing suitable species of fish (native and exotic) for rearing in cages in the southern region of the Caspian Sea. This study from the perspective of economic efficiency and maintaining ecology were analyzed. The results showed that the southern Caspian Sea has the ability to aquaculture fish. However, due to the yearly temperature variations of water (from surface to a depth of 50 meters), the talent of aquaculture area is preferred for fish in cold water than warm water fish due to possibility rearing over the years. The survey showed that the current conditions and due to unfavorable changes in the ecology of the area in the last decade, the use of non-native fish culture in cages is not recommended. Many species of native fish are suitable for rearing in cages. Nevertheless, there is no infrastructure suitable for the production of all of them. Therefore, respectively species of Salmo caspius, Huso huso and Common carp of Caspian Sea for cultivation of in cages was suggested. In the current situation, this fish for their ability to grow faster than the need for selectivity. It is noteworthy that detailed environmental assessments and species risk assessments before the final introduction of any fish species (native and exotic) for rearing in cages in the Caspian Sea ecosystem is essential

Identification and characterization of pathogenic viruses in aquatic animal using advanced techniques in order to develop rapid diagnostic kit (shrimp viral disease kit as well as TSV, HPV and MBV )

Shrimp production increasing rapidly in the world and in2013 the production reaches 4.2 MT. In Iran the shrimp production is under development and estimated in 1393, 20 thousand tons produced. In this regards the important subject is health and disease in shrimp farm. The white spot syndrome virus for second time appears in chabahar and damage many farms. Because the aquaculture activity expand in the world in national, regional and international scale, many emerge disease are endanger. In this regard the viral disease is very important and not only decrease the production but also has a side effect in business and national economy. For control and prevention the viral disease, the accurate methods such as PCR kit were developed. In this project the PCR methods with sensitivity, specificity and efficacy was designed and used for detection viral disease. Many viruses have several serotypes and in different area maybe new serotype induce the disease. For this reason, the specific kit will be design. Three viruses consist of MBV, TSV and IHHNV are very pathogenic in shrimp farm and need the specific PCR kit for detection them. In this project the MBV virus was identified and designs a new primer with Oligo software and the primer amplified a part of DNA with 185 bp in the gel. The specificity and sensitivity of primer were checked by IQ2000 Kit and the primer used for detection unknown samples

Survey on health status in aquaculture sturgeons centers (Mazandaran, Guilan And Golestan Provinces)

Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus, Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×10^5, 4.8±0.5×10^5 per mm^3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm^3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p>0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm^3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm^3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05)

Estimating global injuries morbidity and mortality: methods and data used in the Global Burden of Disease 2017 study

BACKGROUND: While there is a long history of measuring death and disability from injuries, modern research methods must account for the wide spectrum of disability that can occur in an injury, and must provide estimates with sufficient demographic, geographical and temporal detail to be useful for policy makers. The Global Burden of Disease (GBD) 2017 study used methods to provide highly detailed estimates of global injury burden that meet these criteria. METHODS: In this study, we report and discuss the methods used in GBD 2017 for injury morbidity and mortality burden estimation. In summary, these methods included estimating cause-specific mortality for every cause of injury, and then estimating incidence for every cause of injury. Non-fatal disability for each cause is then calculated based on the probabilities of suffering from different types of bodily injury experienced. RESULTS: GBD 2017 produced morbidity and mortality estimates for 38 causes of injury. Estimates were produced in terms of incidence, prevalence, years lived with disability, cause-specific mortality, years of life lost and disability-adjusted life-years for a 28-year period for 22 age groups, 195 countries and both sexes. CONCLUSIONS: GBD 2017 demonstrated a complex and sophisticated series of analytical steps using the largest known database of morbidity and mortality data on injuries. GBD 2017 results should be used to help inform injury prevention policy making and resource allocation. We also identify important avenues for improving injury burden estimation in the future

Epidemiological study on some environmental and management parameters affecting on WSD occurrence in Fenneropenaeus indicus and Penaeus vannamei

For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease. Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas