Effect of Nitrogen and Phosphorus Application on Productivity, Nutrient Uptake and Quality of Teosinte (\u3cem\u3eZea mexicana\u3c/em\u3e L.) Fodder

Teosinte (Zea mexicana L.) is popularly known as Makchari being a close relative of maize. It provides succulent, palatable and nutritive fodder during Kharif season for feeding the animals as green fodder or conserved fodder in the form of silage. Teosinte is an excellent multicut fodder which gives high yield of nutritious green lush fodder in 65-70 days with less inputs as compared to maize. Nitrogen is an essential component of proteins, nucleic acid, enzymes, coenzymes, chlorophyll and cell wall. Phosphorus plays a vital role in crop production as it is involved in CO2 fixation, sugar metabolism, energy storage and transfer. Nutrient deficiency along with imbalanced and non-judicious fertilizers use of the important limiting factor that may affect the yield and quality of teosinte forage. In India about 62% and 49% soils are deficient in nitrogen and phosphorus (Gibson, 2006). The application of nitrogen and phosphorus is considered to be the most important which improves the yield and quality of fodder. The present investigation was under taken to assess the effect of N and P application on productivity, nutrient uptake and quality of teosinte fodder

Incidence and diversity of the fungal genera Aspergillus and Penicillium in Portuguese almonds and chestnuts

Almonds (Prunus dulcis (Miller) D.A. Webb) and European (sweet) chestnuts (Castanea sativa Miller) are of great economic and social impact in Mediterranean countries, and in some areas they constitute the main income of rural populations. Despite all efforts to control fungal contamination, toxigenic fungi are ubiquitous in nature and occur regularly in worldwide food supplies, and these nuts are no exception. This work aimed to provide knowledge on the general mycobiota of Portuguese almonds and chestnuts, and its evolution from field to the end of storage. For this matter, 45 field chestnut samples and 36 almond samples (30 field samples and six storage samples) were collected in Trás-os-Montes, Portugal. All fungi belonging to genus Aspergillus were isolated and identified to the section level. Fungi representative of other genera were identified to the genus level. In the field, chestnuts were mainly contaminated with the genera Fusarium, Cladosporium, Alternaria and Penicillium, and the genus Aspergillus was only rarely found, whereas almonds were more contaminated with Aspergillus. In almonds, Aspergillus incidence increased significantly from field to the end of storage, but diversity decreased, with potentially toxigenic isolates belonging to sections Flavi and Nigri becoming more significant and widespread throughout storage. These fungi were determined to be moderately associated, which can be indicative of mycotoxin co-contamination problems if adequate storage conditions are not secured.P. Rodrigues was supported by grants SFRH/BD/28332/2006 from Fundacao para a Ciencia e a Tecnologia (FCT), and SFRH/PROTEC/49555/2009 from FCT and Polytechnic Institute of Braganca, Portugal

Food, Nutrition and Agrobiodiversity Under Global Climate Change

Available evidence and predictions suggest overall negative effects on agricultural production as a result of climate change, especially when more food is required by a growing population. Information on the effects of global warming on pests and pathogens affecting agricultural crops is limited, though crop–pest models could offer means to predict changes in pest dynamics, and help design sound plant health management practices. Host-plant resistance should continue to receive high priority as global warming may favor emergence of new pest epidemics. There is increased risk, due to climate change, to food and feed contaminated by mycotoxin-producing fungi. Mycotoxin biosynthesis gene-specific microarray is being used to identify food-born fungi and associated mycotoxins, and investigate the influence of environmental parameters and their interactions for control of mycotoxin in food crops. Some crop wild relatives are threatened plant species and efforts should be made for their in situ conservation to ensure evolution of new variants, which may contribute to addressing new challenges to agricultural production. There should be more emphasis on germplasm enhancement to develop intermediate products with specific characteristics to support plant breeding. Abiotic stress response is routinely dissected to component physiological traits. Use of transgene(s) has led to the development of transgenic events, which could provide enhanced adaptation to abiotic stresses that are exacerbated by climate change. Global warming is also associated with declining nutritional quality of food crops. Micronutrient-dense cultivars have been released in selected areas of the developing world, while various nutritionally enhanced lines are in the release pipeline. The high-throughput phenomic platforms are allowing researchers to accurately measure plant growth and development, analyze nutritional traits, and assess response to stresses on large sets of individuals. Analogs for tomorrow’s agriculture offer a virtual natural laboratory to innovate and test technological options to develop climate resilience production systems. Increased use of agrobiodiversity is crucial to coping with adverse impacts of global warming on food and feed production and quality. No one solution will suffice to adapt to climate change and its variability. Suits of technological innovations, including climate-resilient crop cultivars, will be needed to feed 9 billion people who will be living in the Earth by the middle of the twenty-first century

Safety, immunogenicity, and reactogenicity of BNT162b2 and mRNA-1273 COVID-19 vaccines given as fourth-dose boosters following two doses of ChAdOx1 nCoV-19 or BNT162b2 and a third dose of BNT162b2 (COV-BOOST): a multicentre, blinded, phase 2, randomised trial

Background Some high-income countries have deployed fourth doses of COVID-19 vaccines, but the clinical need, effectiveness, timing, and dose of a fourth dose remain uncertain. We aimed to investigate the safety, reactogenicity, and immunogenicity of fourth-dose boosters against COVID-19.Methods The COV-BOOST trial is a multicentre, blinded, phase 2, randomised controlled trial of seven COVID-19 vaccines given as third-dose boosters at 18 sites in the UK. This sub-study enrolled participants who had received BNT162b2 (Pfizer-BioNTech) as their third dose in COV-BOOST and randomly assigned them (1:1) to receive a fourth dose of either BNT162b2 (30 µg in 0·30 mL; full dose) or mRNA-1273 (Moderna; 50 µg in 0·25 mL; half dose) via intramuscular injection into the upper arm. The computer-generated randomisation list was created by the study statisticians with random block sizes of two or four. Participants and all study staff not delivering the vaccines were masked to treatment allocation. The coprimary outcomes were safety and reactogenicity, and immunogenicity (antispike protein IgG titres by ELISA and cellular immune response by ELISpot). We compared immunogenicity at 28 days after the third dose versus 14 days after the fourth dose and at day 0 versus day 14 relative to the fourth dose. Safety and reactogenicity were assessed in the per-protocol population, which comprised all participants who received a fourth-dose booster regardless of their SARS-CoV-2 serostatus. Immunogenicity was primarily analysed in a modified intention-to-treat population comprising seronegative participants who had received a fourth-dose booster and had available endpoint data. This trial is registered with ISRCTN, 73765130, and is ongoing.Findings Between Jan 11 and Jan 25, 2022, 166 participants were screened, randomly assigned, and received either full-dose BNT162b2 (n=83) or half-dose mRNA-1273 (n=83) as a fourth dose. The median age of these participants was 70·1 years (IQR 51·6–77·5) and 86 (52%) of 166 participants were female and 80 (48%) were male. The median interval between the third and fourth doses was 208·5 days (IQR 203·3–214·8). Pain was the most common local solicited adverse event and fatigue was the most common systemic solicited adverse event after BNT162b2 or mRNA-1273 booster doses. None of three serious adverse events reported after a fourth dose with BNT162b2 were related to the study vaccine. In the BNT162b2 group, geometric mean anti-spike protein IgG concentration at day 28 after the third dose was 23 325 ELISA laboratory units (ELU)/mL (95% CI 20 030–27 162), which increased to 37 460 ELU/mL (31 996–43 857) at day 14 after the fourth dose, representing a significant fold change (geometric mean 1·59, 95% CI 1·41–1·78). There was a significant increase in geometric mean anti-spike protein IgG concentration from 28 days after the third dose (25 317 ELU/mL, 95% CI 20 996–30 528) to 14 days after a fourth dose of mRNA-1273 (54 936 ELU/mL, 46 826–64 452), with a geometric mean fold change of 2·19 (1·90–2·52). The fold changes in anti-spike protein IgG titres from before (day 0) to after (day 14) the fourth dose were 12·19 (95% CI 10·37–14·32) and 15·90 (12·92–19·58) in the BNT162b2 and mRNA-1273 groups, respectively. T-cell responses were also boosted after the fourth dose (eg, the fold changes for the wild-type variant from before to after the fourth dose were 7·32 [95% CI 3·24–16·54] in the BNT162b2 group and 6·22 [3·90–9·92] in the mRNA-1273 group).Interpretation Fourth-dose COVID-19 mRNA booster vaccines are well tolerated and boost cellular and humoral immunity. Peak responses after the fourth dose were similar to, and possibly better than, peak responses after the third dose

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Not AvailableAn experiment consisting of four irrigation water salinity levels (good quality water (0.69 dS/m), 2 EC, 4 EC and 6 EC water) and four N levels (0, 50, 100 and 125% recommended dose of N) was laid down in a factorial RBD to find out the effect on green fodder yield and quality of fodder oats. Salinity of irrigation water upto 4 EC did not influence fodder yield. The levels of proximate principles except those of total ash, organic matter and ether extract at 2nd cut were not affected significantly by irrigation salinity up to 6 EC compared to good quality water. Application of 100% recommended dose (150 kg ha-1) of N (RDN) significantly increased the yield and quality of oats green fodder. N application did not significantly affect hemicellulose, neutral detergent fibre at 2nd cut and acid detergent fibre at 1st cut. The treatment involving 125% RDN decreased NDF content significantly while ADF and total carbohydrate content decreased significantly using 50% RDN. Crude protein content also increased significantly using 50% RDN.Not Availabl

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Not AvailableA field experiment was conducted during the rainy (June - October) and winter (November – March) seasons for two years during 2010-2012 at research farm of ICAR - Indian Agricultural Research Institute, New Delhi, India. The experiment was laid out in a randomized block design consisting of seven treatments and three replication. Results revealed the integrated application of 90 kg K ha-1 supplemented through muriate of potash (MOP) @ 60 kg K and farmyard manure (FYM) @ 30 kg K resulted in highest grain yield of 4.93 t ha-1 and biological yield of 11.71 t ha-1 in maize. Similarly, highest grain yield of 5.44 t ha-1 and biological yield of 14.03 t ha-1 were observed in treatment applied with integrated dose of K @ 90 kg K ha-1 supplemented through MOP and FYM in wheat crop. The highest uptake of K was observed under treatment applied through 30 kg K through MOP + 30 kg K through FYM in both cropping cycles. Nutrient use efficiency of N, P and K increased by K application. In soil, decline was observed in different fractions of K irrespective of different applied K treatments. All the treatments where K was added showed a negative balance of K availability in soil. Application of 60 kg K ha-1 through MOP in both the season showed minimum negative K balance (-281.3 kg ha-1). Integrated K application @ 90 Kg K ha-1 resulted in highest exchangeable and nonexchangeable K in the soil for both seasons. To achieve a sustained yield level, nutrient use efficiency and soil fertility integrated K application @ 60 kg K ha-1 for the rainy season in maize and a dose of 60 kg K ha-1 through MOP alone in the winter season for wheat crop may be recommended.Not Availabl