Imprinted Polymers with Affinity for Phosphorylated Peptides and Proteins

The present invention relates to a method of separating or extracting phosphorylated amino acids, peptides or proteins with a molecularly imprinted polymer and to the preparation of said molecularly imprinted polymer as well as the use of molecularly imprinted polymer for separating or extracting phosphorylated amino acids, peptides or proteins

Exploiting dense shell/packing principles to invoke stereoselectivity in a reaction accelerated by a chiral dendrimer

As dendrimers approach their dense shell or dense packed limit, a certain amount of conformational organization exists. Any substrate binding within the dendrimer’s external layerwill experience the same organizational effects. This paper describes how these effects can be exploited towards stereocontrol with respect to binding and reactivity

An enantioselective imprinted receptor for Z-glutamate exhibiting a binding induced color change

New MIP for the specific recongnition of oxyanions and in particular of dihydrofolate reductase inhibitors such as methotrexate. The strong binding is detected by a colour change and this advocates applications in the field of sensors for the recognition of molecules of pharmaceutical interest. The recognition of oxyanions can also be exploited for the selective analysis of peptides and proteins such as biopharmaceuticals. Nuovo polimero per l’analisi qualitativa (riconoscimento specifico) di ossianioni, in particolare di inibitori della diidrofolatoreduttasi come il metotrexato. La presenza di legame forte, dovuto ad una interazione stechiometrica monomero-templato, è visibile ad occhio nudo (intenso colore giallo) fenomeno interessante applicazioni nel campo dei sensori per molecole di interesse farmaceutico. Si preconizza di estendere l’uso di tale polimero anche nel riconoscimento di molecole di interesse biologico (peptidi, piccole proteine) che contengono la funzione ossianionica

Imprinted polymers displaying high affinity for sulfated protein fragments

Leaving a lasting impression: Molecularly imprinted polymers (MIPs) featuring neutral binding sites for phosphotyrosine (P) is also shown to have affinity for sulfotyrosine (S) and a sulfotyrosine-containing peptide. The MIPs were capable of selectively capturing both a phosphorylated and a sulfated peptide in a mixture and could release the peptides separately based on elution conditions