O Império contra-ataca: um manifesto pós-transexual

In this essay considered to be the founding landmark of American transgender studies, Sandy Stone, analyzing autobiographies of trans women and medical literature, examines and challenges the way in which medical knowledge has historically investigated and cultivated normative conceptions about transsexuality. The author points out cisheteronormative assumptions that guided the production of knowledge by medical professionals about gender variance and connects this field of research and intervention with trans-exclusionary feminist discourses in a fundamental aspect: the thought of trans people as alienated and incapable of understanding and expressing truth about themselves. If at first it can be read as a response to the anti-trans book “The Transsexual Empire” by Janice Raymond, “The Empire Strikes Back” proves to be more than that: it is a critique of the exclusion of trans people from the regime of enunciation about trans subjectivity and experience, and a call for such subjects to appropriate themselves of their transformative and disruptive capacity, participating in the formation of a counter-discursive intellectual, creative and political movement.En este ensayo considerado el hito fundacional de los estudios trans estadounidenses, Sandy Stone, a partir del análisis de autobiografías de mujeres trans y de la literatura médica, examina y cuestiona la forma con que el conocimiento médico ha históricamente investigado y desarrollado concepciones normativas sobre la transexualidad. La autora señala suposiciones cisheteronormativas que guiaron la producción de conocimiento por parte de los profesionales médicos sobre el tránsito de género, y conecta este campo de investigación e intervención con los discursos feministas radicales en un aspecto fundamental: el entendimiento de las personas trans como alienadas e incapaces de comprender y expresar la verdad. acerca de si mismas. Si en principio se puede leerlo como una respuesta al libro anti-trans “El imperio transexual” de Janice Raymond, “El imperio contraataca” resulta ser más que eso: es una crítica a la exclusión de las personas trans del régimen de enunciación sobre subjetividades y experiencias trans, y un llamado a que dichos sujetos se apropien de su capacidad transformadora y disruptiva, participando en la formación de un movimiento intelectual, creativo y político contradiscursivo.Neste ensaio considerado o marco fundador dos estudos trans estadunidenses, Sandy Stone, a partir da análise de autobiografias de mulheres trans e de literatura médica, examina e desafia o modo como saberes médicos historicamente investigaram e elaboraram concepções normativas sobre a transexualidade. A autora aponta pressupostos cisheteronormativos que orientaram a produção de conhecimento de profissionais da medicina acerca do trânsito de gênero, e conecta esse campo de pesquisa e intervenção com discursos de feministas radicais em um cerne fundamental: a apreensão de pessoas trans como alienadas e incapazes de compreender e expressar verdade sobre si. Se em um primeiro momento pode ser lido como uma resposta ao livro anti-trans “The Transsexual Empire” de Janice Raymond, “O império contra-ataca” se mostra mais do que isso: é uma crítica à exclusão de pessoas trans do regime de enunciação sobre subjetividades e experiência trans, e um chamamento para que tais sujeitos se apropriem de sua capacidade transformativa e disruptiva, participando na formação de um movimento intelectual, criativo e político contra-discursivo

The Binary White Dwarf LHS 3236

The white dwarf LHS 3236 (WD1639+153) is shown to be a double-degenerate binary, with each component having a high mass. Astrometry at the U.S. Naval Observatory gives a parallax and distance of 30.86 +/- 0.25 pc and a tangential velocity of 98 km/s, and reveals binary orbital motion. The orbital parameters are determined from astrometry of the photocenter over more than three orbits of the 4.0-year period. High-resolution imaging at the Keck Observatory resolves the pair with a separation of 31 and 124 mas at two epochs. Optical and near-IR photometry give a set of possible binary components. Consistency of all data indicates that the binary is a pair of DA stars with temperatures near 8000 and 7400 K and with masses of 0.93 and 0.91 M_solar; also possible, is a DA primary and a helium DC secondary with temperatures near 8800 and 6000 K and with masses of 0.98 and 0.69 M_solar. In either case, the cooling ages of the stars are ~3 Gyr and the total ages are <4 Gyr. The combined mass of the binary (1.66--1.84 M_solar) is well above the Chandrasekhar limit; however, the timescale for coalescence is long.Comment: Accepted for the Astrophysical Journa

The USNO-B Catalog

USNO-B is an all-sky catalog that presents positions, proper motions, magnitudes in various optical passbands, and star/galaxy estimators for 1,042,618,261 objects derived from 3,643,201,733 separate observations. The data were obtained from scans of 7,435 Schmidt plates taken for the various sky surveys during the last 50 years. USNO-B1.0 is believed to provide all-sky coverage, completeness down to V = 21, 0.2 arcsecond astrometric accuracy at J2000, 0.3 magnitude photometric accuracy in up to five colors, and 85% accuracy for distinguishing stars from non-stellar objects. A brief discussion of various issues is given here, but the actual data are available from http://www.nofs.navy.mil and other sites.Comment: Accepted by Astronomical Journa

Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted nanopore sequencing

More than 50 neurological and neuromuscular diseases are caused by short tandem repeat (STR) expansions, with 37 different genes implicated to date. We describe the use of programmable targeted long-read sequencing with Oxford Nanopore's ReadUntil function for parallel genotyping of all known neuropathogenic STRs in a single assay. Our approach enables accurate, haplotype-resolved assembly and DNA methylation profiling of STR sites, from a list of predetermined candidates. This correctly diagnoses all individuals in a small cohort (n = 37) including patients with various neurogenetic diseases (n = 25). Targeted long-read sequencing solves large and complex STR expansions that confound established molecular tests and short-read sequencing and identifies noncanonical STR motif conformations and internal sequence interruptions. We observe a diversity of STR alleles of known and unknown pathogenicity, suggesting that long-read sequencing will redefine the genetic landscape of repeat disorders. Last, we show how the inclusion of pharmacogenomic genes as secondary ReadUntil targets can further inform patient care

Deception studies manipulating centrally acting performance modifiers: a review.

Athletes anticipatorily set and continuously adjust pacing strategies before and during events to produce optimal performance. Selfregulation ensures maximal effort is exerted in correspondence with the end point of exercise, while preventing physiological changes that are detrimental and disruptive to homeostatic control. The integration of feedforward and feedback information, together with the proposed brain_s performance modifiers is said to be fundamental to this anticipatory and continuous regulation of exercise. The manipulation of central, regulatory internal and external stimuli has been a key focus within deception research, attempting to influence the self-regulation of exercise and induce improvements in performance. Methods of manipulating performance modifiers such as unknown task end point, deceived duration or intensity feedback, self-belief, or previous experience create a challenge within research, as although they contextualize theoretical propositions, there are few ecological and practical approaches which integrate theory with practice. In addition, the different methods and measures demonstrated in manipulation studies have produced inconsistent results. This review examines and critically evaluates the current methods of how specific centrally controlled performance modifiers have been manipulated, within previous deception studies. From the 31 studies reviewed, 10 reported positive effects on performance, encouraging future investigations to explore the mechanisms responsible for influencing pacing and consequently how deceptive approaches can further facilitate performance. The review acts to discuss the use of expectation manipulation not only to examine which methods of deception are successful in facilitating performance but also to understand further the key components used in the regulation of exercise and performance

A programme theory for liaison mental health services in England

Background: Mechanisms by which liaison mental health services (LMHS) may bring about improved patient and organisational outcomes are poorly understood. A small number of logic models have been developed, but they fail to capture the complexity of clinical practice. Method: We synthesised data from a variety of sources including a large national survey, 73 in-depth interviews with acute and liaison staff working in hospitals with different types of liaison mental health services, and relevant local, national and international literature. We generated logic models for two common performance indicators used to assess organisational outcomes for LMHS: response times in the emergency department and hospital length of stay for people with mental health problems. Results: We identified 8 areas of complexity that influence performance, and 6 trade-offs which drove the models in different directions depending upon the balance of the trade-off. The logic models we developed could only be captured by consideration of more than one pass through the system, the complexity in which they operated, and the trade-offs that occurred. Conclusions: Our findings are important for commissioners of liaison services. Reliance on simple target setting may result in services that are unbalanced and not patient-centred. Targets need to be reviewed on a regular basis, together with other data that reflect the wider impact of the service, and any external changes in the system that affect the performance of LMHS, which are beyond their control

Interleukin-15 Plays a Central Role in Human Kidney Physiology and Cancer through the γc Signaling Pathway

The ability of Interleukin-15 (IL-15) to activate many immune antitumor mechanisms renders the cytokine a good candidate for the therapy of solid tumors, particularly renal cell carcinoma. Although IL-15 is being currently used in clinical trials, the function of the cytokine on kidney's components has not been extensively studied; we thus investigated the role of IL-15 on normal and tumor renal epithelial cells. Herein, we analyzed the expression and the biological functions of IL-15 in normal renal proximal tubuli (RPTEC) and in their neoplastic counterparts, the renal clear cell carcinomas (RCC). This study shows that RPTEC express a functional heterotrimeric IL-15Rαβγc complex whose stimulation with physiologic concentrations of rhIL-15 is sufficient to inhibit epithelial mesenchymal transition (EMT) commitment preserving E-cadherin expression. Indeed, IL-15 is not only a survival factor for epithelial cells, but it can also preserve the renal epithelial phenotype through the γc-signaling pathway, demonstrating that the cytokine possess a wide range of action in epithelial homeostasis. In contrast, in RCC in vitro and in vivo studies reveal a defect in the expression of γc-receptor and JAK3 associated kinase, which strongly impacts IL-15 signaling. Indeed, in the absence of the γc/JAK3 couple we demonstrate the assembly of an unprecedented functional high affinity IL-15Rαβ heterodimer, that in response to physiologic concentrations of IL-15, triggers an unbalanced signal causing the down-regulation of the tumor suppressor gene E-cadherin, favoring RCC EMT process. Remarkably, the rescue of IL-15/γc-dependent signaling (STAT5), by co-transfecting γc and JAK3 in RCC, inhibits EMT reversion. In conclusion, these data highlight the central role of IL-15 and γc-receptor signaling in renal homeostasis through the control of E-cadherin expression and preservation of epithelial phenotype both in RPTEC (up-regulation) and RCC (down-regulation)

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

Human matrix metalloproteinases (MMPs) belong to the M10 family of the MA clan of endopeptidases. They are ubiquitarian enzymes, structurally characterized by an active site where a Zn(2+) atom, coordinated by three histidines, plays the catalytic role, assisted by a glutamic acid as a general base. Various MMPs display different domain composition, which is very important for macromolecular substrates recognition. Substrate specificity is very different among MMPs, being often associated to their cellular compartmentalization and/or cellular type where they are expressed. An extensive review of the different MMPs structural and functional features is integrated with their pathological role in several types of diseases, spanning from cancer to cardiovascular diseases and to neurodegeneration. It emerges a very complex and crucial role played by these enzymes in many physiological and pathological processes