69 research outputs found
Antibacterial Properties of Marine Sponges from Mauritius Waters
Purpose: To investigate the antimicrobial activity of crude and fractionated extracts of Biemna tubulosa and Stylissa spp. thriving in Trou aux Biches lagoon, North East Mauritius.Methods: Crude extracts (methanol: dichloromethane 1:1) and fractions of Biemna tubulosa and Stylissa spp (hexane, ethyl acetate and butanol) were tested against some bacterial organisms, namely, Escherichia coli, Staphylococcus aureus and Enterococcus faecalis, using broth microdilution assay. The extracts were screened for the presence of compounds that could be responsible for bioactivity.Results: Preliminary chemical screening revealed the presence of tannins, saponins, terpenes, alkaloids and phenols. The hexane fraction of Stylissa spp. and the ethyl actetate and butanol extracts of Biemna tubulosa were more active than the control antibiotic. Maximum antimicrobial activity was noted for the butanol fraction of Biemna tubulosa with minimum inhibitory concentration (MIC) of 0.091 mg/ml against E. coli. The hexane soluble fraction of Stylissa spp. was more active with MIC of 0.125 mg/ml against S. aureus and E. coli and 0.250 mg/ml against E. faecalis.Conclusion: The broad spectrum antibacterial activity of these sponges seemed to be due to the presence of sesterterpenes and triterpenes, alkaloids and tannins detected in the extract fractions. This is probably the first report on the antimicrobial activity of Biemna tubulosa and Stylissa spp. from Mauritius waters.Keywords: Biemna tubulosa, Stylissa spp., Antibacterial activity, Marine sponges, MIC
Ferroelectric Solitons Crafted in Epitaxial Bismuth Ferrite Superlattices
In ferroelectrics, complex interactions among various degrees of freedom
enable the condensation of topologically protected polarization textures. Known
as ferroelectric solitons, these particle-like structures represent a new class
of materials with promise for beyond CMOS technologies due to their ultrafine
size and sensitivity to external stimuli. Such polarization textures have
scarcely been reported in multiferroics. Here, we report a range of soliton
topologies in bismuth ferrite strontium titanate superlattices. High-resolution
piezoresponse force microscopy and Cs-corrected high-angle annular dark-field
scanning transmission electron microscopy reveal a zoo of topologies, and
polarization displacement mapping of planar specimens reveals center-convergent
and divergent topological defects as small as 3 nm. Phase field simulations
verify that some of these topologies can be classed as bimerons, with a
topological charge of plus and minus one, and first-principles-based effective
Hamiltonian computations show that the co-existence of such structures can lead
to non-integer topological charges, a first observation in a BiFeO3-based
system. Our results open new opportunities in multiferroic topotronics
Registration of ICGV-SM 85048 Peanut Germplasm
ICGV-SM 85048 (Reg. no. GP-90, PI 598134), an improved
Spanish peanut (Arachis hypogaea L. subsp.fastigiata var. vitlgaris)
germplasm, was developed at the SADC/ICRISAT (Southern African
Development Community/International Crops Research Institute
for the Semi-Arid Tropics) Groundnut Project in Malawi. It
was first tested at the Mauritius Sugar Industry Research Institute
(MSIRI), Mauritius, in 1988. After evaluations in on-station and
on-farm trials, it was released in 1992 as 'Stella' in Mauritius (8).
It is suitable for cultivation both in sugarcane (Sacchanim officinarum
L.) interrows and in pure stand
Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis
Bacterial vaginosis is a genital tract infection, thought to be caused by transformation of a lactobacillus-rich flora to a dysbiotic microbiota enriched in mixed anaerobes. The most prominent of these is Gardnerella vaginalis (GV), an anaerobic pathogen that produces sialidase enzyme to cleave terminal sialic acid residues from human glycans. Notably, high sialidase activity is associated with preterm birth and low birthweight. We explored the potential of the sialidase inhibitor Zanamavir against GV whole cell sialidase activity using methyl-umbelliferyl neuraminic acid (MU-NANA) cleavage assays, with Zanamavir causing a 30% reduction in whole cell GV sialidase activity (p < 0.05). Furthermore, cellular invasion assays using HeLa cervical epithelial cells, infected with GV, demonstrated that Zanamivir elicited a 50% reduction in cell association and invasion (p < 0.05). Our data thus highlight that pharmacological sialidase inhibitors are able to modify BV-associated sialidase activity and influence host-pathogen interactions and may represent novel therapeutic adjuncts
Expression of transforming growth factor-beta-1 and p27Kip1 in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
Target strength of skipjack tuna (Katsuwanus pelamis) associated with fish aggregating devices (FADs)
[EN] This paper presents measures of target strength (TS; dB re 1 m(2)) and models of TS vs. fork length (L; cm), i.e. TS = 20log(L) + b(20), for skip-jack tuna associated with fish aggregating devices (FADs) in the Central Pacific Ocean. Measurements were made using 38-, 120-, and 200-kHz split-beam echosounders on a purse-seine workboat during fishing operations. To mitigate potential bias due to unresolved targets, TS measurements were rejected if they were not simultaneously detected with multiple echosounder frequencies in approximately the same location. The filtered TS and concomitantly sampled L data were used to estimate b(20) = -76, -71, and -70.5 dB for 38, 120, and 200 kHz, respectively, using the method of least squares. For comparison, quasi-independent estimates of TS and b(20) were calculated from acoustic echo-integration and catch data representing entire aggregations around the FADs. The results differed by <= 1 dB for all three frequencies. The sensitivities of these results to variations in fish morphology and behaviour were explored using a simulation of TS for fish without swimbladders. The utility of the results on acoustic properties of skipjack tuna and next research steps to achieve selective fishing at FADs are discussed.We thank the following organizations and people for their support of this work: the governments of Kiribati, Tuvalu, and Tokelau which permitted this research in their EEZs; Albacora for allowing this work aboard F/V ALBATUN TRES; Fishing Master Euken Mujika; the captain and crew; the scientists and divers J. Filmalter and F. Forget are thanked for invaluable insight about fish behaviour, vertical stratification and non-target species composition at FADs; Hector Pena for providing instruction on the sonar setup and analysis; Yolanda Lacalle for the illustration in Figure 2; and Andres Uriarte for advice concerning transmission of statistical errors. The research reported in the present document was funded by the International Seafood Sustainability Foundation (ISSF) and conducted independently by the authors. The report and its results, professional opinions and conclusions are solely the work of the authors. This paper is contribution 843 from AZTI (Marine or Food Research).Boyra, G..; Moreno, G.; Sobradillo, B.; Pérez Arjona, I.; Sancristóbal, I.; Demer, D. (2018). Target strength of skipjack tuna (Katsuwanus pelamis) associated with fish aggregating devices (FADs). ICES Journal of Marine Science. 75(5):1790-1802. https://doi.org/10.1093/icesjms/fsy041S1790180275
Large expert-curated database for benchmarking document similarity detection in biomedical literature search
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency–Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research
Large expert-curated database for benchmarking document similarity detection in biomedical literature search
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe
Identification of lipolytic enzymes isolated from bacteria indigenous to Eucalyptus wood species for application in the pulping industry
This study highlights the importance of determining substrate specificity at variable experimental conditions. Lipases and esterases were isolated from microorganisms cultivated from Eucalyptus wood species and then concentrated (cellulases removed) and characterized. Phenol red agar plates supplemented with 1% olive oil or tributyrin was ascertained to be the most favourable method of screening for lipolytic activity. Lipolytic activity of the various enzymes were highest at 45–61 U/ml at the optimum temperature and pH of between at 30–35 °C and pH 4–5, respectively. Change in pH influenced the substrate specificity of the enzymes tested. The majority of enzymes tested displayed a propensity for longer aliphatic acyl chains such as dodecanoate (C12), myristate (C14), palmitate (C16) and stearate (C18) indicating that they could be characterised as potential lipases. Prospective esterases were also detected with specificity towards acetate (C2), butyrate (C4) and valerate (C5). Enzymes maintained up to 95% activity at the optimal pH and temperature for 2–3 h. It is essential to test substrates at various pH and temperature when determining optimum activity of lipolytic enzymes, a method rarely employed. The stability of the enzymes at acidic pH and moderate temperatures makes them excellent candidates for application in the treatment of pitch during acid bi-sulphite pulping, which would greatly benefit the pulp and paper industry
Co-occurring mycotoxins in animal feeds
Mycotoxin contamination of feeds results in economic loss and transmission of toxins in the food chain. Animal feeds, the raw ingredients used in their manufacture, namely, maize, wheat, sunflower seeds, cottonseeds, bagasse, wheaten bran, gluten feed and pet foods from South Africa were surveyed for contaminating mycotoxin-producing fungi and their toxins: aflatoxins, fumonisins, zearalenone andochratoxins. Toxins were extracted and analysed by high performance liquid chromatography and fluorometry. Twenty-one of the twenty-three samples were contaminated by Aspergillus flavus which cooccurredwith A. parasiticus in two samples, A. tamarii in seven and Fusarium moniliforme in one. Rhizopus stolonifer, R. oryzae and yeast were also isolated. Aflatoxins were detected in seventeen samples, fumonisin in six and zearalenone in three. Aflatoxin levels ranged between 0.8 ± 0.2 and 156 ± 8 ìg/kg (ppb), zearalenone between 100 ± 10.5 and 165 000 ± 200 ìg/kg and fumonisin B1 between 15 ± 3.0 and 5 900 ± 40ìg/kg. Ochratoxins were not detected in any of the samples. In most countries worldwide, legislated levels for aflatoxins and patulin are 20 ìg/kg and 50 ìg/kg, respectively, for human foods. Fumonisins,zearalenone and other toxins are not legislated in most of the countries. Ten of the feeds contain toxin levels above legislated limits (for Canada and the USA) and guidelines set by other countries. The results ofthis study highlight the need for mycotoxin legislation in the animal feed industry
- …