53 research outputs found
Interaction of phonons at superfluid helium-solid interfaces
A new method of obtaining the interaction Hamiltonian of phonons at
superfluid helium-solid interface is proposed in the work. Equations of
hydrodynamic variables are obtained in terms of second quantization if helium
occupies a half-space. The contributions of all processes to the heat flux from
solid to superfluid helium are calculated based on the obtained Hamiltonian.
The angular distribution of phonons emitted by a solid is found in different
processes. It is shown that all the exit angles of superfuild helium phonons
are allowed. The obtained results are compared with experimental data and with
previous theoretical works.Comment: 9 pages, 3 figure
Pressure of thermal excitations in superfluid helium
We find the pressure, due to the thermal excitations of superfluid helium, at
the interface with a solid. The separate contributions of phonons, rotons
and rotons are derived. The pressure due to rotons is shown to be
negative and partially compensates the positive contribution of rotons,
so the total roton pressure is positive but several times less than the
separate and roton contributions. The pressure of the quasiparticle
gas is shown to account for the fountain effect in . An experiment is
proposed to observe the negative pressure due to rotons.Comment: 14 pages, 4 figure
The effectiveness of biofilm formation of daily cultures of clinically significant strains of opportunistic bacteria
Background. The formation of biofilm structures by microorganisms living in the hospital environment is a serious medical problem. To conduct correct experimental studies, it is necessary to know the speed and efficiency of biofilm formation by clinically significant species of opportunistic bacteria. Aim: to study the kinetics of plankton culture growth and the rate of biofilm formation by clinically significant pathogens of infections associated with medical care to substantiate the methodology of further research. Materials and methods. The strains from the working collection of the Laboratory for Microbiome and Microecology of the Scientific Сentre for Family Health and Human Reproduction Problems were used. Experiments were carried out with conditionally pathogenic microorganisms of the Enterobacteriaceae family and non-fermenting gram-negative bacteria. The optical density was measured, the total microbial number of the cell suspension was determined, and the morphological structure of the biofilm was evaluated using a light microscope on sterile cover glasses for thespecies Pseudomonas aeruginosa, Klebsiella pneumoniae and Serratia marcescens. Results. The duration of the lag phase of the kinetic curve of cell growth varied in isolates of S. marcescens, P. aeruginosa and K. pneumoniae from 1 to 4 and 6 hours of cultivation, respectively. Despite this, the exponential growth phase was the same for all tested isolates and amounted to 4 hours. Thus, isolates of clinically significant species entered the stationary growth phase after 5–10 hours of cultivation and were characterized as fast-growing. On abiotic surfaces, after 8 hours of incubation of the tested cultures, the initial stages of the formation of biofilm structures were observed, after 20 hours the formed multilayer biofilm was visualized, after 24 hours succession occurred, new single cells were attached to the place of the detached structures. Conclusion. The data obtained on the duration of the main stages of growth kinetics compared with the visualization of the formation of biofilm structures on abiotic surfaces should be taken into account when studying the effects of disinfectants, antiseptics and antibacterial drugs on planktonic cells and biofilm associations of clinically significant opportunistic microorganisms
Effects of аntimicrobials on <i>Pseudomonas aeruginosa</i> biofilm formation
Pseudomonas aeruginosa is one of the most problematic pathogens in medical institutions, which may be due to the ability of this microorganism to exist in a biofilm, which increases its resistance to antimicrobials, as well as its prevalence and survival ability in the external environment. This work aimed to evaluate the antimicrobial susceptibility of P. aeruginosa strains in planktonic and biofilm forms. We studied 20 strains of P. aeruginosa collected during 2018–2021 by specialists from the Laboratory of Microbiome and Microecology of the Scientific Centre for Family Health and Human Reproduction Problems. The identification of strains was carried out using test systems for differentiating gram-negative non-fermenting bacteria (NEFERMtest 24 Erba Lachema s.r.o., Czech Republic), and confirmed by mass spectrometric analysis and 16S rRNA gene sequencing. Antimicrobial activity was assessed by the degree of inhibition of cell growth in planktonic and biofilm forms (on a flat-bottomed 96-well plastic immunological plate). All clinical isolates of P. aeruginosa were biofilm formers, 47.6 % of the isolates were weak biofilm formers, and 52.4 % of the isolates were moderate biofilm formers. Planktonic cells and the forming biofilm of the tested P. aeruginosa strains were carbapenems-resistant. Biofilm formation was suppressed in more than 90 % of cases by the agents of the cephalosporin and aminoglycoside groups. Antimicrobial susceptibility of P. aeruginosa strains in the formed biofilm was significantly lower (p < 0.05). Carbapenems and cephalosporins did not affect the mature biofilms of the tested P. aeruginosa strains in more than 60 % of cases. Only non-beta-lactam antibiotics (ciprofloxacin and amikacin) suppressed the growth of planktonic cells and destroyed the mature biofilm. The revealed differences in the effect of the tested antimicrobials on the P. aeruginosa strains biofilms correlate with resistance to a number of antibiotics. To prevent biofilm formation in the hospital strains of P. aeruginosa, the use of ceftazidime may be recommended, and antimicrobials such as ciprofloxacin and amikacin may be used to affect mature biofilms of P. aeruginosa
Frequency of strains with multiple antibiotic resistance in the structure of opportunitistic pathogens
Background. The problem of antibiotic resistance has remained significant for the medical community for more than half a century, since the first cases of resistance to penicillin were registered. The aim. Analysis of the long-term dynamics of changes in the antibacterial resistance of microorganisms and the creation of a collection of multi-resistant strains of opportunistic microorganisms. Materials and methods. The study included data from 3173 bacteriological samples of various loci of the human body for 2010 and 2020–2021. The sensitivity of isolated cultures was determined by the disk diffusion method to antimicrobial drugs of the following groups: penicillins, cephalosporins, fluoroquinolones, aminoglycosides, carbapenems, tetracyclines, macrolides, lincosamides, oxazolidinones, glycopeptides and others. Results. In the general structure of conditionally pathogenic microorganisms, a significant increase in the frequency of isolation of multidrug-resistant representatives of the genus Staphylococcus by two or more times was observed in 2021 compared to 2010 and 2020. We also observed a significant increase in the proportion of multidrug-resistant Streptococcus spp. and non-fermenting gram-negative bacteria. These changes marked the beginning of the creation of a collection of conditionally pathogenic microorganisms with multiple antibacterial resistance. In the structure of multiresistant microorganisms included in the “Collection of human microbiota of the Irkutsk region”, the leading positions belong to Klebsiella pneumoniae (23.81 %), Escherichia coli (19.05 %) and Staphylococcus aureus (22.22 %). Conclusion. Antibiotic resistance monitoring is an important measure to control the resistance of community-acquired and nosocomial (nosocomial) microorganisms both within a particular country and globally
Measurement of the Bottom-Strange Meson Mixing Phase in the Full CDF Data Set
We report a measurement of the bottom-strange meson mixing phase \beta_s
using the time evolution of B0_s -> J/\psi (->\mu+\mu-) \phi (-> K+ K-) decays
in which the quark-flavor content of the bottom-strange meson is identified at
production. This measurement uses the full data set of proton-antiproton
collisions at sqrt(s)= 1.96 TeV collected by the Collider Detector experiment
at the Fermilab Tevatron, corresponding to 9.6 fb-1 of integrated luminosity.
We report confidence regions in the two-dimensional space of \beta_s and the
B0_s decay-width difference \Delta\Gamma_s, and measure \beta_s in [-\pi/2,
-1.51] U [-0.06, 0.30] U [1.26, \pi/2] at the 68% confidence level, in
agreement with the standard model expectation. Assuming the standard model
value of \beta_s, we also determine \Delta\Gamma_s = 0.068 +- 0.026 (stat) +-
0.009 (syst) ps-1 and the mean B0_s lifetime, \tau_s = 1.528 +- 0.019 (stat) +-
0.009 (syst) ps, which are consistent and competitive with determinations by
other experiments.Comment: 8 pages, 2 figures, Phys. Rev. Lett 109, 171802 (2012
Evolution of a pulse of noninteracting quasiparticles with dispersion and initial angular width
The evolution of a pulse of noninteracting quasiparticles, caused by their different velocities
and angular distribution of momenta, is studied theoretically. Equations are found that describe
the shape of the pulse surface at any time. The time of the beginning, end and duration of the density
of the quasiparticle energy flux is determined at a general spatial point. The quasiparticle energy
density is considered at all times and positions, and it is shown that the region of high energy
density, in the middle of the pulse, is equal to the initial energy density under certain conditions.
These theoretical results are discussed in relation to experimental data on the evolution of a pulse
of noninteracting phonons in superfluid helium
Epizootic Activity of Natural Plague Foci in the Territory of the Russian Federation in 2011, and Prognosis for 2012
Carried out is the assessment of epizootic activity of natural plague foci in the Russian Federation and in the Republic of Kazakhstan during the period of 2000-2010. It is specified that 38 plague microbe strains have been isolated in the territory of the Russian Federation, the total area of epizooties being 882,5 km2. Plague microbe strains are isolated in the territory of East-Caucasian high-mountain and Altai mountain natural foci. Identified are the reasons of low epizootic activity of plain and low-mountain natural plague foci in the Russian Federation. Outlined is the role of climatic factors in the development of current intense depression in numbers of carriers and vectors of plague. Put forward is the prognosis on the low epizootic activity of the natural plague foci in the Russian Federation in 2012. It is substantiated that the emergence of local epizooties in 2012 is to take place in the territory of Altai mountain, Tuvin mountain and East-Caucasian high-mountain natural plague foci
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