Regulation of amino acid and nucleotide metabolism by crustacean hyperglycemic hormone in the muscle and hepatopancreas of the crayfish Procambarus clarkia.

To comprehensively characterize the metabolic roles of crustacean hyperglycemic hormone (CHH), metabolites in two CHH target tissues of the crayfish Procambarus clarkii, whose levels were significantly different between CHH knockdown and control (saline-treated) animals, were analyzed using bioinformatics tools provided by an on-line analysis suite (MetaboAnalyst). Analysis with Metabolic Pathway Analysis (MetPA) indicated that in the muscle Glyoxylate and dicarboxylate metabolism, Nicotinate and nicotinamide metabolism, Alanine, aspartate and glutamate metabolism, Pyruvate metabolism, and Nitrogen metabolism were significantly affected by silencing of CHH gene expression at 24 hours post injection (hpi), while only Nicotinate and nicotinamide metabolism remained significantly affected at 48 hpi. In the hepatopancreas, silencing of CHH gene expression significantly impacted, at 24 hpi, Pyruvate metabolism and Glycolysis or gluconeogenesis, and at 48 hpi, Glycine, serine and threonine metabolism. Moreover, analysis using Metabolite Set Enrichment Analysis (MSEA) showed that many metabolite sets were significantly affected in the muscle at 24hpi, including Ammonia recycling, Nicotinate and nicotinamide metabolism, Pyruvate metabolism, Purine metabolism, Warburg effect, Citric acid cycle, and metabolism of several amino acids, and at 48 hpi only Nicotinate and nicotinamide metabolism, Glycine and serine metabolism, and Ammonia recycling remained significantly affected. In the hepatopancreas, MSEA analysis showed that Fatty acid biosynthesis was significantly impacted at 24 hpi. Finally, in the muscle, levels of several amino acids decreased significantly, while those of 5 other amino acids or related compounds significantly increased in response to CHH gene silencing. Levels of metabolites related to nucleotide metabolism significantly decreased across the board at both time points. In the hepatopancreas, the effects were comparatively minor with only levels of thymine and urea being significantly decreased at 24 hpi. The combined results showed that the metabolic effects of silencing CHH gene expression were far more diverse than suggested by previous studies that emphasized on carbohydrate and energy metabolism. Based on the results, metabolic roles of CHH on the muscle and hepatopancreas are suggested: CHH promotes carbohydrate utilization in the hepatopancreas via stimulating glycolysis and lipolysis, while its stimulatory effect on nicotinate and nicotinamide metabolism plays a central role in coordinating metabolic activity in the muscle with diverse and wide-ranging consequences, including enhancing the fluxes of glycolysis, TCA cycle, and pentose phosphate pathway, leading to increased ATP supply and elevated protein and nucleic acid turnovers

Evaluation of Spatial and Temporal Performances of ERA-Interim Precipitation and Temperature in Mainland China

ERA-Interim has been widely considered as a valid proxy for observations at global and regional scales. However, the verifications of ERA-Interim precipitation and temperature in mainland China have been rarely conducted, especially in the spatial and long-term performances. Therefore, in this study, we employed the interpolated ground station (STA) data to evaluate the spatial and temporal patterns and trends of ERA-Interim precipitation and temperature during 1980-2012. The results showed that relatively weaker performances were observed in ERA-Interim precipitation, with the skill score (S index) ranging from 0.41 to 0.50. Interannual ERA-Interim precipitation presented comparable trends with STA precipitation at the annual and seasonal scales. Spatial patterns of empirical orthogonal function (EOF) modes and corresponding principal components were evidently different between annual ERA-Interim and STA precipitation. For temperature, annual and seasonal patterns of ERA-Interim data were in good consistency with those of STA over China with the S index ranging from 0.59 to 0.70. Yet interannual STA temperature recorded stronger warming trends (from 0.37K decade(-1) of wintertime to 0.53 Kdecade(-1) of springtime) at the annual and seasonal scales compared to corresponding periods for ERA-Interim temperature (from 0.03Kdecade 21 of wintertime to 0.25Kdecade(-1) of summertime). Overall, ERA-Interim precipitation and temperature had good agreement with STA data in east China with lower elevation (< 1000m above sea level), but good agreements were not observed in west China with higher elevation. The findings suggest that caution should be paid when using ERA-Interim precipitation and temperature in areas with complex orography

Spatio-Temporal Evolution of Sandy Land and its Impact on Soil Wind Erosion in the Kubuqi Desert in Recent 30 Years

Continuous remote-sensing monitoring of sand in desert areas and the exploration of the spatio–temporal evolution characteristics of soil–wind erosion has an important scientific value for desertification prevention and ecological restoration. In this study, the Kubuqi Desert was selected as the study area, and the Landsat series satellite remote sensing data, supplemented by satellite remote sensing data such as GE images, SPOT-5, ZY-3, GF-1/2/6, etc., integrated object-oriented, decision tree, and auxiliary human–computer interaction interpretation methods, developed the Kubuqi Desert area dataset from 1990 to 2020, and established a soil erosion intensity database of the past 30 years based on the soil–wind erosion correction equation. The results show that the application of the training samples obtained by a high-score collaborative ground sampling to land use/cover classification in desert areas can effectively improve the efficiency of remote-sensing mapping of sand changes and the accuracy of change information identification, and the overall accuracy of the classification results is 95%. In general, the sandy area of the Kubuqi Desert area has decreased year by year, during which the mobile sand in the hinterland of the desert has expanded in a scattered distribution. The overall soil–wind erosion intensity showed a downward trend, especially since 2000; the ecological improvement trend after the implementation of desertification control projects is obvious. Changes in the sand type contributed the most to the reduction of soil–wind erosion intensity (contribution 81.14%), ecological restoration played a key role in reducing the soil–wind erosion intensity (contribution 14.42%), and the increase of forest and grass vegetation covers and agricultural oases played a positive role in solidifying the soil- and wind-proof sand fixation. The pattern of sandy land changes in desert areas is closely related to the national ecological civilization construction policy and the impact of climate change

Critical role of interleukin (IL)-17 in inflammatory and immune disorders: An updated review of the evidence focusing in controversies

Interleukin 17 (IL-17) is a proinflammatory cytokine that has been the focus of intensive research because of its crucial role in the pathogenesis of different diseases across many medical specialties. In this context, the present review in which a panel of 13 experts in immunology, dermatology, rheumatology, neurology, hematology, infectious diseases, hepatology, cardiology, ophthalmology and oncology have been involved, puts in common the mechanisms through which IL-17 is considered a molecular target for the development of novel biological therapies in these different fields. A comprehensive review of the literature and analysis of the most outstanding evidence have provided the basis for discussing the most relevant data related to IL-17A blocking agents for the treatment of different disorders, such as psoriasis, psoriatic arthritis, rheumatoid arthritis, ankylosing spondylitis, cardiovascular disorders, non alcoholic fatty liver disease, multiple sclerosis, inflammatory bowel disease, uveitis, hematological and solid cancer. Current controversies are presented giving an opening line for future research.This work was supported by Novartis Pharmaceuticals Spain

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe

Maximum Gross Primary Productivity Dominates the Trend in Gross Primary Productivity in China’s Deciduous Forest Ecosystems

The terrestrial gross primary productivity (GPP) has increased over the past two decades. However, the climatic attribution and the physiological and phenological processes that control the trends in the GPP are still unclear. Here, we used remote-sensing-based vegetation GPP and phenology datasets, analyzed the spatial and temporal variation in the GPP, investigated the influence of the growing season length (GSL) and the maximum value of gross primary productivity (GPPmax) on the annual GPP, and quantified the effect of climate variables on the annual GPP. Our results identified a significant increase in the annual GPP (11.97 gC/m2/yr) during 2001–2020 in China’s deciduous forest. The GPPmax trend dominated the trends in the GPP, when compared with the GSL. Moreover, climate warming in summer contributes to the increase in the GPP and the GPPmax, while the extension of the GSL is primarily due to the temperature rise in spring. The annual GPP of the planted forest showed a higher increasing rate than the natural forest, due to the significant enhancement of the GPPmax and the high sensitivity of the GSL to climatic factors in the planted forest. Our findings provide a new perspective on the phenological and physiological causes of the trends in the GPP, and emphasize the importance of capturing the variability in the GPPmax when modeling the GPP

Recent advances in visible-light-driven photoelectrochemical water splitting: catalyst nanostructures and reaction systems

Photoelectrochemical (PEC) water splitting using solar energy has attracted great attention for generation of renewable hydrogen with less carbon footprint, while there are enormous challenges that still remain for improving solar energy water splitting efficiency, due to limited light harvesting, energy loss associated to fast recombination of photogenerated charge carriers, as well as electrode degradation. This overview focuses on the recent development about catalyst nanomaterials and nanostructures in different PEC water splitting systems. As photoanode, Au nanoparticle-decorated TiO2 nanowire electrodes exhibited enhanced photoactivity in both the UV and the visible regions due to surface plasmon resonance of Au and showed the largest photocurrent generation of up to 710 nm. Pt/CdS/CGSe electrodes were developed as photocathode. With the role of p–n heterojunction, the photoelectrode showed high stability and evolved hydrogen continuously for more than 10 days. Further, in the Z-scheme system (Bi2S3/TNA as photoanode and Pt/SiPVC as photocathode at the same time), a self-bias (open-circuit voltage V oc = 0.766 V) was formed between two photoelectrodes, which could facilitate photogenerated charge transfers and enhance the photoelectrochemical performance, and which might provide new hints for PEC water splitting. Meanwhile, the existing problems and prospective solutions have also been reviewed

The Effect of Humidity on Dielectric Properties of PP-Based Nano-Dielectric

Nano-dielectrics are sensitive to humidity and easily degraded in damp environment because of the high surface energy of nanoparticles. In order to study the effect of humidity on the dielectric properties of nano-dielectric, polypropylene (PP) was modified by polyolefin elastomer (POE) and nano-SiO2, and the samples with obvious filling concentration were pre-selected by breakdown strength for damp aging. The aging experiments were carried out in different relative humidity. The dielectric properties of new, hygroscopic saturation and samples after drying were measured and analyzed. It is found that the breakdown strength of hygroscopic saturation nano-dielectrics decreased obviously compared with new samples, and it was difficult to recover after drying. The damp degradation resulted in different changing trends of permittivity of PP and nano-dielectric, but there were relaxation loss peaks of water in both of them. The influence of damp degradation on the trap distribution was studied by thermally stimulated depolarization currents (TSDC), and it was found that the traps level introduced by water molecules was different in PP and nano-dielectrics. All experiment results showed that the performance of nano-dielectrics degraded obviously in humid environment, and it was difficult to recover even after complete drying because of the existence of bounded water molecules in nano-dielectrics

Multi-Scale Analysis of Green Space for Human Settlement Sustainability in Urban Areas of the Inner Mongolia Plateau, China

Green space in intra-urban regions plays a significant role in improving the human habitat environment and regulating the ecosystem service in the Inner Mongolian Plateau of China, the environmental barrier region of North China. However, a lack of multi-scale studies on intra-urban green space limits our knowledge of human settlement environments in this region. In this study, a synergistic methodology, including the main process of linear spectral decomposition, vegetation-soil-impervious surface area model, and artificial digital technology, was established to generate a multi-scale of green space (i.e., 15-m resolution intra-urban green components and 0.5-m resolution park region) and investigate multi-scale green space characteristics as well as its ecological service in 12 central cities of the Inner Mongolian Plateau. Results showed that: (1) Total urban areas and urban green space across the studied cities were 1249.87 km2 and 295.40 km2, indicating that the average proportion of green space to urban areas was 24.03%. (2) The proportion of green space to urban areas ranged from 17.09% to 32.17%, and the proportion of parks’ green space to green space ranged from 5.55% to 50.20%, indicating a wide range of quantitative discrepancies. (3) In different climate regions, there were higher proportions of urban/park green space in arid/semi-arid areas to reduce the impacts of dry climate on human settlements; by contrast, lower green space in humid areas mainly displayed a scattered pattern because of the relatively lower influence of climate pressure. (4) Green coverage was an essential indicator of the “Beautiful China” project, and its ratio within 500-m ecological service zones from parks across all cities was 46.14%, which indicated that the ratio of residential land and green space was close to 1:1. Overall, urban/park green space patterns in urban areas adapted to the different climate features in the Inner Mongolian Plateau. For better human settlement sustainability across all studied cities, more greening patches and ecological corridors should be designed in the lower green space regions of the Inner Mongolian Plateau