Chemical polymorphism of the essential oils from populations of Thymus caespititius grown on the island S. Jorge (Azores)

The compositions of the essential oils obtained from ten populations of Thymus caespititius grown on S. Jorge (Azores) were studied by GC and GC-MS. All the oils analysed were dominated by their monoterpene fraction (69-91%). The sesquiterpene fraction was rather small (4-17%) and consisted mainly of oxygen-containing compounds (3-13%). Enantiomeric ratio of sabinene and [alpha]-terpineol was investigated in the oils from some populations. (+)-Sabinene was detected with high enatiomeric purity (96-99%). Although (+)-[alpha] terpineol was the predominant enantiomer, its purity varied considerably (66-97%). Cluster analysis of all identified components grouped the oils into four main clusters that corresponded with their major components: [alpha]-terpineol (43-68%), carvacrol (32-52%), thymol (44-58%) and sabinene (41%). The essential oil from Th. caespititius showed a clear chemical polymorphism, that was particularly evident among four populations growing along a 200 m distance at Pico Verde.http://www.sciencedirect.com/science/article/B6TH7-41K2349-C/1/cb06975bf5d34c6355b8edd36cafe7f

Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens

6 pags., 2 figs., 1 tab.Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (H, C and N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.This work was supported by the following Fundação para a Ciência e a Tecnologia (FCT-MCTES) Grants: PTDC/BIA-BQM/31981/2017 (to CAS) and scholarship Grant No. PD/BD/114445/2016 (to LRT) through Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012, UID/FIS/00068/2013 (CEFITEC). This work was also supported by Unidade de Ciências Biomoleculares Aplicadas-UCIBIO which is fnanced by national funds from FCT/MEC (UID/Multi/04378/2013) and co-fnanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We acknowledge the use of the “Manuel Rico” NMR laboratory, LMR (CSIC), a Spanish large-scale national NMR facility ICTS R-LRB

Transverse momentum spectra of charged particles in proton-proton collisions at 1as=900 GeV with ALICE at the LHC

The inclusive charged particle transverse momentum distribution is measured in proton-proton collisions at s=900 GeV at the LHC using the ALICE detector. The measurement is performed in the central pseudorapidity region (|\u3b7|<0.8) over the transverse momentum range 0.15<10 GeV/c. The correlation between transverse momentum and particle multiplicity is also studied. Results are presented for inelastic (INEL) and non-single-diffractive (NSD) events. The average transverse momentum for |\u3b7|<0.8 is \u3008pT\u3009INEL=0.483\ub10.001 (stat.)\ub10.007 (syst.) GeV/c and \u3008pT\u3009NSD=0.489\ub10.001 (stat.)\ub10.007 (syst.) GeV/c, respectively. The data exhibit a slightly larger \u3008pT\u3009 than measurements in wider pseudorapidity intervals. The results are compared to simulations with the Monte Carlo event generators PYTHIA and PHOJET. \ua9 2010